Highlights
- Simple: Quickly and easily rescue plasmid from yeast.
- Efficient Isolation: Works well with low-copy and hard-to-isolate plasmids.
- High-Quality: Isolated plasmid DNA is ideal for molecular biology techniques, such as PCR, transformation, hybridization, etc.
Description
Applicable For | Plasmid DNA is well suited for downstream applications such as PCR, transformation, hybridization and other sensitive applications |
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Elution Volume | ≥ 10 µl per well |
Equipment | Centrifuge with microplate carriers |
Processing Volume | ≤ 1.5 ml of Culture |
Sample Source | Cell Culture (Colonies/Patches or Liquid Culture) |
Size Range | Up to 25 kb. |
Yield | Typically between 0.01-0.3 ng for most 2 µ based plasmids from 1.5 ml overnight cultures |
Q1: What is the difference between Zymoprep Yeast Plasmid Miniprep I and Miniprep II?
Both the Zymoprep Yeast Plasmid Miniprep I and II utilize the same chemistry for lysis; however, Miniprep I uses isopropanol precipitation and Miniprep II utilizes a column for purification. The Miniprep II allows for consistent yield and purity; and samples can be concentrated to a low elution volume.
Q2: What is the typical plasmid yield?
Typically, between 0.01 - 0.3 ng for most 2 µ based plasmid from 1.5 ml overnight cultures.In order to generate more plasmid, the plasmid is typically transformed into E. Coli, cultured, and isolated using a traditional E. Coli plasmid prep.
Q3: Can this kit be used to isolate linear plasmid DNA?
Yes
Q4: If I’m using stationary phase yeast cells, what can I do to improve sample lysis?
We generally recommend working with fresh or early log phase cells, which are easier to lyse. For stationary phase cells, user optimization is necessary, and we recommend increasing digestion to > 1 hour and/ or increasing the amount of Zymolyase.
Q5: What yeast strains are these kits compatible with?
Any strains susceptible to Zymolyase, which includes the following fungal genera: Asbya KloekeraCandidaKluyveromycesDebaryomycesLipomycesEremotheciumMetschikowiaEndomycesPichiaHansenulaPullulariaHanseniasporaSaccharomycesSaccaromycodesSaccharomycopsisSchizosaccahromycesTorulopsis
Cat # | Name | Size | Price | |
---|---|---|---|---|
D2004-1-45 | Solution 1 Digestion Buffer | 45 ml | $88.00 | |
D2004-1-90 | Solution 1 Digestion Buffer | 90 ml | $146.00 | |
D2004-2-45 | Solution 2 Lysis Buffer | 45 ml | $88.00 | |
D2004-2-90 | Solution 2 Lysis Buffer | 90 ml | $146.00 | |
D2004-3-180 | Solution 3 Neutralizing Buffer | 180 ml | $146.00 | |
D2004-3-90 | Solution 3 Neutralizing Buffer | 90 ml | $88.00 | |
D4003-2-24 | DNA Wash Buffer (Concentrate) | 24 ml | $33.00 | |
D4003-2-48 | DNA Wash Buffer (Concentrate) | 48 ml | $60.00 | |
C1001-50 | Collection Tubes | 50 Pack | $15.00 | |
C2002 | Collection Plate | 2 Plates | $22.00 | |
C2003 | Elution Plate | 2 Plates | $19.00 | |
C2004 | Zymo-Spin I-96 Plate | 2 Plates | $142.00 | |
P1001-2 | 96-Well Block | 2 Blocks | $18.00 | |
C2007-8 | 96-Well Plate Cover Foil | 8 Foils | $18.00 | |
C2011-2 | Air Permeable Sealing Cover | 2 Pack | $12.00 | |
C2011-4 | Air Permeable Sealing Cover | 4 Pack | $24.00 | |
C2011-8 | Air Permeable Sealing Cover | 8 Pack | $42.00 |
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一、机械裂解法主要有以下两中:
1.热休克(Thermal shock),既反复冻溶法,是一种常用的机械裂解方式比,通常由冷冻和解冻两部分组成(freezing and thawing),.原理:由于细胞内冰粒形成和剩余细胞液的盐浓度增高引起溶胀,使细胞结构破碎.冷冻通常在液氮或-20°C冰上进行,解冻可以在37、50、65 或100℃水浴中进热休克比化学裂解温和,但是很有效,有资料表明用热休克和溶菌酶与SDS的方法获得了90%的细胞裂解率.
2.超声波处理(Ultrasonication)既利用超声加热的方法,把细胞破碎.但这种处理会导致DNA 的断裂,所以加热不宜过剧烈,要设定好超声时间和间隙时间,一般超声时间不超过5秒,间隙时间最好大于超声时间,这些都有利于保护酶的活性.bead-beating 也是常用的机械处理方式,有报道指出bead-beating 比热休克和化学裂解的细胞裂解效果更好 虽然DNA产量较高,但通常得到的DNA片段较小.
二、 化学裂解和酶裂解法(在提核酸时联合使用)
主要是裂解液处理法,细胞裂解液的主要目的有以下几种:(1)利用去污剂破坏脂质双分子层,破裂细胞;(2)溶解蛋白;(3)蛋白变性使其稳定;(4)抑制蛋白酶活性.
主要根据不同的目的,裂解液的组成有所不同,主要有提取核酸和蛋白两中.在提取RNA或DNA时,我们主要是要充分裂解细胞,得到更多的核酸;如果我们的目的是蛋白,那要根据蛋白的位置、特性等因素考虑裂解液,在提取蛋白后,再根据实验需要复性蛋白等.以下是细胞裂解中常用试剂和其作用:
50 mM Tris-HCl pH 7.4(缓冲体系),150 mM NaCl(等渗体系),1 mM PMSF (强大的蛋白酶抑制剂),1 mM EDTA(变性剂和稳定剂),5 μg/ml Aprotinin(蛋白酶抑制剂),5 μg/ml Leupeptin(蛋白酶抑制剂),1% Triton x-100(破坏细胞),1% Sodium deoxycholate(中度变性剂和蛋白溶解剂),0.1% SDS(强变性剂和蛋白溶解剂).7M 尿素,2M硫脲(可以提高膜蛋白的融解),蛋白酶K等.
这样可不可以?
另还有几个问题:1、这样的裂解液裂解线粒体提蛋白可不可以
2、裂解液加多少剂量
3、12000g离心10min和30分钟有没有区别
不知单纯使用冰浴中超声裂解够了吗?还是说要选用裂解液呢?最主要的就是我不知道该怎么赔这个裂解液,使其COX活性不产生影响
一、机械裂解法主要有以下两中:
1.热休克(Thermal shock),既反复冻溶法,是一种常用的机械裂解方式比,通常由冷冻和解冻两部分组成(freezing and thawing),.原理:由于细胞内冰粒形成和剩余细胞液的盐浓度增高引起溶胀,使细胞结构破碎.冷冻通常在液氮或-20°C冰上进行,解冻可以在37、50、65 或100℃水浴中进热休克比化学裂解温和,但是很有效,有资料表明用热休克和溶菌酶与SDS的方法获得了90%的细胞裂解率.
2.超声波处理(Ultrasonication)既利用超声加热的方法,把细胞破碎.但这种处理会导致DNA 的断裂,所以加热不宜过剧烈,要设定好超声时间和间隙时间,一般超声时间不超过5秒,间隙时间最好大于超声时间,这些都有利于保护酶的活性.bead-beating 也是常用的机械处理方式,有报道指出bead-beating 比热休克和化学裂解的细胞裂解效果更好 虽然DNA产量较高,但通常得到的DNA片段较小.
二、 化学裂解和酶裂解法(在提核酸时联合使用)
主要是裂解液处理法,细胞裂解液的主要目的有以下几种:(1)利用去污剂破坏脂质双分子层,破裂细胞;(2)溶解蛋白;(3)蛋白变性使其稳定;(4)抑制蛋白酶活性.
主要根据不同的目的,裂解液的组成有所不同,主要有提取核酸和蛋白两中.在提取RNA或DNA时,我们主要是要充分裂解细胞,得到更多的核酸;如果我们的目的是蛋白,那要根据蛋白的位置、特性等因素考虑裂解液,在提取蛋白后,再根据实验需要复性蛋白等.以下是细胞裂解中常用试剂和其作用:
50 mM Tris-HCl pH 7.4(缓冲体系),150 mM NaCl(等渗体系),1 mM PMSF (强大的蛋白酶抑制剂),1 mM EDTA(变性剂和稳定剂),5 μg/ml Aprotinin(蛋白酶抑制剂),5 μg/ml Leupeptin(蛋白酶抑制剂),1% Triton x-100(破坏细胞),1% Sodium deoxycholate(中度变性剂和蛋白溶解剂),0.1% SDS(强变性剂和蛋白溶解剂).7M 尿素,2M硫脲(可以提高膜蛋白的融解),蛋白酶K等.