RecombinantHumanIL-4ProteinSummary
| AdditionalInformation | ANewrhIL-4isNowAvailable!Thenewproteinismammaliancellexpressed! |
| DetailsofFunctionality | MeasuredinacellproliferationassayusingTF‑1humanerythroleukemiccells.Kitamura,T.etal.(1989)J.CellPhysiol.140:323.TheED50forthiseffectis0.05-0.2ng/mL.ThespecificactivityofrecombinanthumanIL-4isapproximately2.9x104IU/μg,whichiscalibratedagainsthumanIL-4WHOInternationalStandard(NIBSCcode:88/656). |
| Source | E.coli-derivedhumanIL-4proteinHis25-Ser153,withanN-terminalMet |
| Accession# | P05112 |
| N-terminalSequence | Met |
| Protein/PeptideType | RecombinantProteins |
| Gene | IL4 |
| Purity | >97%,bySDS-PAGEunderreducingconditionsandvisualizedbysilverstain. |
| EndotoxinNote | <0.10 eu="" per="" 1="" μg="" of="" the="" protein="" by="" the="" lal="">0.10> |
Applications/Dilutions
| TheoreticalMW | 15kDa.Disclaimernote:Theobservedmolecularweightoftheproteinmayvaryfromthelistedpredictedmolecularweightduetoposttranslationalmodifications,posttranslationcleavages,relativecharges,andotherexperimentalfactors. | |
| SDS-PAGE | 14kDa,reducingconditions | |
| ReviewedApplications |
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| Publications |
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Packaging,Storage&Formulations
| Storage | Useamanualdefrostfreezerandavoidrepeatedfreeze-thawcycles.
|
| Buffer | Lyophilizedfroma0.2μmfilteredsolutioninPBSwithBSAasacarrierprotein. |
| Purity | >97%,bySDS-PAGEunderreducingconditionsandvisualizedbysilverstain. |
| ReconstitutionInstructions | Reconstituteat100-200μg/mLinsterilePBScontainingatleast0.1%humanorbovineserumalbumin. |
Notes
AlternateNamesforRecombinantHumanIL-4Protein
- Bcellgrowthfactor1
- BCDF
- B-cellstimulatoryfactor1
- BCGF1
- BCGF-1
- binetrakin
- BSF1
- BSF-1
- IL4
- IL-4
- IL-4B_cellstimulatoryfactor1
- interleukin4
- interleukin-4
- Lymphocytestimulatoryfactor1
- MGC79402
- pitrakinra
Background
Interleukin-4(IL-4),alsoknownasBcell-stimulatoryfactor-1,isamonomeric,approximately13kDa‑18kDaTh2cytokinethatshowspleiotropiceffectsduringimmuneresponses(1‑3).Itisaglycosylatedpolypeptidethatcontainsthreeintrachaindisulfidebridgesandadoptsabundledfouralpha-helixstructure(4).HumanIL-4issynthesizedwitha24aasignalsequence.Alternatesplicinggeneratesanisoformwitha16aainternaldeletion.MaturehumanIL-4shares55%,39%and43%aasequenceidentitywithbovine,mouse,andratIL-4,respectively.Human,mouse,andratIL-4arespecies-specificintheiractivities(5‑7).IL-4exertsitseffectsthroughtworeceptorcomplexes(8,9).ThetypeIreceptor,whichisexpressedonhematopoieticcells,isaheterodimeroftheligandbindingIL-4Ralphaandthecommongammachain(asharedsubunitofthereceptorsforIL-2,-7,-9,-15,and‑21).ThetypeIIreceptoronnonhematopoieticcellsconsistsofIL-4RalphaandIL‑13Ralpha1.ThetypeIIreceptoralsotransducesIL-13mediatedsignals.IL-4isprimarilyexpressedbyTh2-biasedCD4+Tcells,mastcells,basophils,andeosinophils(1,2).Itpromotescellproliferation,survival,andimmunoglobulinclassswitchtoIgG4andIgEinhumanBcells,acquisitionoftheTh2phenotypebynaïveCD4+Tcells,primingandchemotaxisofmastcells,eosinophils,andbasophils,andtheproliferationandactivationofepithelialcells(10‑13).IL-4playsadominantroleinthedevelopmentofallergicinflammationandasthma(12,14).
- Benczik,M.andS.L.Gaffen(2004)Immunol.Invest.33:109.
- Chomarat,P.andJ.Banchereau(1998)Int.Rev.Immunol.17:1.
- Yokota,T.etal.(1986)Proc.Natl.Acad.Sci.83:5894.
- Redfield,C.etal.(1991)Biochemistry30:11029.
- Ramirez,F.etal.(1988)J.Immunol.Meth.221:141.
- Leitenberg,D.andT.L.Feldbush(1988)Cell.Immunol.111:451.
- Mosman,T.R.etal.(1987)J.Immunol.138:1813.
- Mueller,T.D.etal.(2002)Biochim.Biophys.Acta1592:237.
- Nelms,K.etal.(1999)Annu.Rev.Immunol.17:701.
- Paludan,S.R.(1998)Scand.J.Immunol.48:459.
- Corthay,A.(2006)Scand.J.Immunol.64:93.
- Ryan,J.J.etal.(2007)Crit.Rev.Immunol.27:15.
- Grone,A.(2002)Vet.Immunol.Immunopathol.88:1.
- Rosenberg,H.F.etal.(2007)J.AllergyClin.Immunol.119:1303.
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患者,双手扶墙,脚尖着地;医生做好消毒,戴上手套,利用放血针(可以利用测血糖的放血针),在委中穴点刺放血,利用拔罐器把瘀血抽出来,直到见到新鲜血液为止。委中穴是解毒大穴。
中医强调治症不治病,虽然身体还有病毒,但是,只要症状能够解除,不影响正常生活和工作,就可以了。
需要强调:医生,一定要做好消毒、保护,防止病毒感染。 另外,可以把艾滋病作为一种疫病,尝试五苓散;五苓散是用来治疗疫病的。
核心抗原由183个或185个氨基酸组成,高度磷酸化,是乙肝病毒核心颗粒的唯一结构蛋白。正由于它存在于Dane颗粒核心结构表面,被表面抗原覆盖,故不易在血循环中检出。核心抗原具有强免疫原性,可诱导很强的体液免疫和细胞免疫,刺激机体产生抗-HBc。
e抗原为可溶性蛋白质,传染性强,游离存在于血液中,虽然很早就被发现,在病理上认为是HBV复制以具有强感染性的一个指标,但其功能尚不清楚。抗-HBe的出现,是预后良好的征象。向左转|向右转
主要是中度以上细胞免疫缺陷包括:CD4+T淋巴细胞耗竭,外周血淋巴细胞显著减少,CD4<200/μl,CD4/CD8<1.0,(正常人为1.25~2.1),迟发型变态反应皮试阴性,有丝分裂原刺激反应低下。NK细胞活性下降。
2.各种致病性感染的病原体检查
如用PCR方法检测相关病原体,恶性肿瘤的组织病理学检查。
3.HIV抗体检测
采用酶联免疫吸附法、明胶颗粒凝集试验、免疫荧光检测法、免疫印迹检测法、放射免疫沉淀法等,其中前三项常用于筛选试验,后二者用于确证试验。
4.PCR技术检测HIV病毒。
流感病毒的基因组包含________基因,其中__________蛋白可作为__________的结构基础.
我不知道第一空如何填,第二空是否应该填"HA蛋白"?第3空是否应该填"突变"?
多谢各位啊!
新年快乐!
万事如意!
1、存在于细胞外环境时,不显复制活性,但保持感染活性,是病毒体或病毒颗粒形式。
2、抗原,是指能够刺激机体产生(特异性)免疫应答,并能与免疫应答产物抗体和致敏淋巴细胞在体外结合,发生免疫效应(特异性反应)的物质








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