
- Description
- Additional Information
- Readable Documents
- Assay Principle
- Reviews
Introduction
Phosphorus is essential for multiple and diverse biological functions, including cellular signal transduction, mineral meatbolism, and energy exchange. Elevated levels of serum phosphate may be associated with a higher risk of death and adverse cardiovascular outcomes in people with prior myocardial infarction, and is the subject of large amount of research.
Key Benefits
- Quantitation of phosphate anion in blood, plasma and serum samples.
- Sensitive – can detect upto 200nM phosphate
- Easy to use – Fluorescence (ex/em 535/585nm or Absorbance readout @ 570nm)
Additional information
Kit Size | 100 |
---|
Cell Technology’s Phosphate detection kit provides a simple, one-step fluorimetric or colorimetric method for determination of phosphate in serum and plasma samples. The assay is based on an enzyme-coupled reaction that detects inorganic phosphate. The substrate is converted to Hydrogen peroxide in presence of inorganic phosphate in a coupled enzymatic reaction. The Hydrogen Peroxide then reacts with the detection reagent in a 1:1 stoichiometry in presence of Horse Radish Peroxidase to produce the stable fluorescent product.
Substrate + Pi
↓ Coupled enzyme reaction
H2O2
↓ Horse Radish Peroxidase + Detection Reagent
Fluorescent Product
λmax 570nm. λ ex/em 535/585nm.
Colorimetric assay can be read on a spectrophotometer at 570nm.
Fluorescence is measured at excitation 530nm and emission 585nm.
Fig.1 Phosphate standard curve was generated using fluorimetric detection: excitation 535nm and emission 585nm. Incubation time= 60 minutes at 37oC. Standard curve range 1.5625 µM to 100µM. Graph was plotted using 4PL non-linear regression.
Fig.2 Phosphate standard curve inset: R2 =0.9835. Incubation time=60 minutes at 37oC. Standard curve range 1.5625µM to 25µM. Graph plotted with Linear regression.
Document Title |
Fluoro Phosphate Protocol |
Fluoro Phosphate Datasheet |
msds.fluoroPhos |
Reference |
Mohanty, J.G. et al : A highly sensitive fluorescent micro-assay of H2O2 release from activated human leukocytes using a dihydroxyphenoxazine derivative, J.Immunological Methods, 202, issue 2, P.133-141 (1997) |
Tonelli, M. et al : Relation between serum phosphate level and cardiovascular event rate in people with coronary disease, Circulation,112, P.2627-2633 (2005) |
Part# | Reagent | Temperature |
Part # 7023 | 25X Substrate | -20C |
Part # 4025 | Detection Reagent | -20C |
Part # 6026 | Enzyme A | -20C |
Part # 6027 | Enzyme B | -20C |
Part # 3060 | Phosphate Standard | 2-8C |
Part # 6028 | Horseradish Peroxidase Enzyme | 2-8C |
Part # 3059 | 1X Reaction Buffer | 2-8C |
Part # 3061 | Sample Diluent | 2-8C |
1 Black Clear-Bottom 96-Well Plate for Fluorescent Plate Reader |
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1.在手机上下载360手机卫士。
2.下载完成手机卫士之后,在手机中安装360手机卫士。在手机中打开360手机卫士并授予手机卫士 Root 权限,如图所示。
3.在手机卫士主界面打开“应用工具”选项,进入后找到“一键 Root 工具”并打开。如果手机已经获取 Root 权限,手机卫士会提示已经手机获取 Root 权限,可以解除 Root 了。
4.点击“解除 Root”按钮之后,360 Root 工具就会给出提示,“注意!解除 Root 之后将永久失去 Root 权限,并且可能无法重新获取!因此若非送修等特殊需要,不建议解除......”确认后点击“解除 Root”按钮即可解除 Root 权限。
方法二:恢复手机的出厂设置。
1.在待机页面下,点击【应用程序】。
2.点击【设定】。
3.向上滑动手机屏幕,点击【重置】。
4.点击【恢复出厂设定】。
5.点击【重置设备】。
6.点击【全部删除】。
7.完成操作后,待手机自动重启后,手机就成功恢复出厂设定了。
所以说,感染同一种病毒,每个人识别的表位可能不一样。
①乙型肝炎表面抗原—抗体系统(HBsAg/抗—HBs);
②乙型肝炎核心抗原—抗体系统(HBcAg/抗—HBc):
③乙型肝炎e抗原—抗体系统(HBeAg);
④乙型肝炎Dane颗粒抗原—抗体系统
⑤乙型肝炎δ抗原—抗体系统(δ/抗—δ)。
临床意义 1.HBsAg:血清中检测到HBsAg ,表示体内感染了HBV,因而是一种特异性标志。HBsAg阳性见于:①急性乙型肝炎的潜伏期或急性期(大多短期阳性);②HBV致的慢性肝病、迁延性和慢性活动性肝炎、肝炎后肝硬化或原发性肝癌等。③无症状携带者
2.抗HBs:表示曾感染过HBV,不论临床上有无肝炎症状表现,均已得到恢复,并且对HBV有一定的免疫力。
3.HBcAg与抗HBc:由于 HBcAg主要存在于肝细胞核内,并仅存在于Dane颗粒中。因此,对病人血清不能检测HBcAg,而测抗HBc。血清内抗HBc阳性反映:①新近有过HBV感染;②体内有HBV增殖;③有助于诊断急性或慢性乙型肝炎,特别是少数病例就诊时已处于急性恢复期早期,HBsAg已从血中消失,此时血中仅有抗HBc存在,因此,对恢复期患者可作病因追索。
4.HBcAg和抗HBe:HBcAg的存在常表示病人血液有感染性。 HBcAg阳性揭示病人肝脏可能有慢性损害,对预后判断有一定帮助。抗HBe阳性对病人可能有一定的保护力。展开
患者,双手扶墙,脚尖着地;医生做好消毒,戴上手套,利用放血针(可以利用测血糖的放血针),在委中穴点刺放血,利用拔罐器把瘀血抽出来,直到见到新鲜血液为止。委中穴是解毒大穴。
中医强调治症不治病,虽然身体还有病毒,但是,只要症状能够解除,不影响正常生活和工作,就可以了。
需要强调:医生,一定要做好消毒、保护,防止病毒感染。 另外,可以把艾滋病作为一种疫病,尝试五苓散;五苓散是用来治疗疫病的。

