Product Specifications:
Item# 20014: Recombinant T. cruzi (Chagas) Composite Antigen A13,B13,H49,1F8 Antigen Baculo)
Concentration: See vial
Mass/vial: See vial
Volume/vial: See vial
Diluent: 20mM NaPO4, 10mM NaCl, 0.05% Sark, pH8 .
Purity: >95%
Stabilizer: None
Preservative:None
Physical State: Frozen Liquid (-75°C), Lyophilized (RT)
Stability: 3 years
Application: In-Vitro Diagnostics, Immunization.
Description: Recombinant T. cruzi (Chagas) combination A13, B13, H49 and 1F8 antigen expressed in the Baculovirus Expression System.
Purification:This protein is purified by affinity chromatography and by preparative electrophoresis to >95% purity as determined by SDS-PAGE, reduced.
Approx. Molecular Weight: 84kD.
Specificity: This protein binds to T. cruzi (Chagas) converted human serum polyclonal antibodies in ELISA and Western Elisa. Qualified for one-step immunochromatographic diagnostic tests.
Biological Activity: Not done
Application and Instructions for use
Recommended concentrations for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. ELISA and Western ELISA require 10-100ng protein depending on the nature and affinity of the detection reagent. Human serum polyclonal antibodies yield titers of 1:1000 or greater at 100ng of immobilized protein under standard ELISA conditions. Protein concentration for lateral flow rapid diagnostics range from 1-2mg/ml, 1ul/cm.
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主要是中度以上细胞免疫缺陷包括：CD4+T淋巴细胞耗竭，外周血淋巴细胞显著减少，CD4<200/μl，CD4/CD8<1.0，（正常人为1.25～2.1），迟发型变态反应皮试阴性，有丝分裂原刺激反应低下。NK细胞活性下降。
2.各种致病性感染的病原体检查
如用PCR方法检测相关病原体，恶性肿瘤的组织病理学检查。
3.HIV抗体检测
采用酶联免疫吸附法、明胶颗粒凝集试验、免疫荧光检测法、免疫印迹检测法、放射免疫沉淀法等，其中前三项常用于筛选试验，后二者用于确证试验。
4.PCR技术检测HIV病毒。
流感病毒的基因组包含________基因,其中__________蛋白可作为__________的结构基础.
我不知道第一空如何填,第二空是否应该填"HA蛋白"?第3空是否应该填"突变"?
多谢各位啊!
新年快乐!
万事如意!
所以说，感染同一种病毒，每个人识别的表位可能不一样。
核心抗原由183个或185个氨基酸组成，高度磷酸化，是乙肝病毒核心颗粒的唯一结构蛋白。正由于它存在于Dane颗粒核心结构表面，被表面抗原覆盖，故不易在血循环中检出。核心抗原具有强免疫原性，可诱导很强的体液免疫和细胞免疫，刺激机体产生抗-HBc。
e抗原为可溶性蛋白质，传染性强，游离存在于血液中，虽然很早就被发现，在病理上认为是HBV复制以具有强感染性的一个指标，但其功能尚不清楚。抗-HBe的出现，是预后良好的征象。向左转|向右转
