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VivanTechnologies/GF-1 Bacterial DNA Extraction Kit/Pack Size/GF-BA-050
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VivanTechnologies/GF-1 Bacterial DNA Extraction Kit/Pack Size/GF-BA-050
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DescriptionThe GF-1 Bacterial DNA Extraction Kit provides a rapid and efficient method for purification of high molecular weight genomic DNA from either Gram-negative or Gram-positive bacteria. The purification is based on the usage of denaturing agents to provide lysis of cells, denaturation of proteins and subsequently release of genomic DNA. Special buffers provided in the kit are optimized to enhance the binding of DNA onto a specially-treated glass filter membrane for efficient recovery of highly pure genomic DNA.

Features

  • Suitable for both Gram-negative or Gram-positive bacteria
  • Yields up to 20μg of DNA
  • No organic-based extraction required
  • Highly pure genomic DNA ready to use for routine molecular biology applications such as restriction enzyme digestion, PCR, Southern blotting and DNA fingerprinting.

Kit Components

  • Buffer R1
  • Buffer R2
  • Buffer BG
  • Wash Buffer (concentrate)
  • Elution Buffer
  • Proteinase K

Ordering Information

Catalog NoDescriptionPack Size
GF-BA-050GF-1 Bacterial DNA Extraction Kit50 preps
GF-BA-100GF-1 Bacterial DNA Extraction Kit100 preps

DownloadManual

GF-1 Bacterial DNA Extraction Kit

Stability Test Report

GF-1 Bacterial DNA Extraction Kit

PublicationThis Product Has Been Used In: Ekprasert J et al. (2020) Investigating mechanical properties and biocement application of CaCO3, precipitated by a newly-isolated Lysinibacillus sp. WH using artificial neural networks, Scientific Reports,10:16137.Erkihun A & Kausalya R (2020) MCR-1 Gene Encoded Colistin-Resistant Escherichia coli in Raw Chicken Meat and Bean Sprouts in Malaysia, International Journal of Microbiology, Article ID 8853582.Eshamah HL et al. (2020)Extent of pathogenic and spoilage microorganisms in whole muscle meat, meat products and seafood sold in Libyan market, Open Veterinary Journal,10(3):276-288.Fatin Aina Zulkhairi Amin et al. (2020). Probiotic Properties of Bacillus Strains Isolated from Stingless Bee (Heterotrigona itama) Honey Collected across Malaysia,. Int. J. Environ. Res. Public Health, 17, 278.Nonzee H et al. (2020) Interaction Study of Pasteurella Multocida with Culturable Aerobic Bacteria Isolated from Porcine Respiratory Tracts using Coculture in Conditioned Media, Research Square,https://doi.org/10.21203/rs.3.rs-36993/v1Pongrawee N & Neelawan P (2020)Distribution and expression of virulence genes in potentially pathogenic bacteria isolated from seafood in Thailand, CyTA – Journal of Food 18:1, 753-763.Virun Vichaibun & Panan Kanchanaphum (2020) Quantitative LAMP and PCR Detection of Salmonella in Chicken Samples Collected from Local Markets around Pathum Thani Province, Thailand, International Journal of Food Science, Article ID 8833173.in vitro antimicrobial assessment on lactic acid bacteria isolated from common freshwater fishes,. Asia-Pacific Journal of Molecular Biology and Biotechnology, 27(2):18-25.Mankai H et al (2019) Complete Genome Sequence and Methylome Analysis of Thermoactinomyces vulgaris 2H, . Microbiology Resource Announcements, 8:e00657-19.Tung HJ et al. (2019)cDNA-SSR Markers for Molecular Epidemiology of Ganoderma boninense,. , Journal of Oil Palm Research, 31(2):220-237. Boonyayatra, S., Tharavichitkut, P., Oliver, S.P. (2018). Virulence-associated genes and molecular typing of Streptococcus uberis associated with bovine mastitis in northern Thailand, Turkish Journal of Veterinary and Animal Sciences, Vol. 42, 73-81 (2018).Community structures of total bacterial DNA, cultivable bacteria and prototrophs in bulk soil and rhizospheres . Can, H.Y., Elmali, M., Ergün, Y. (2017).Methicillin-resistant Staphylococcus aureus in milk from dairy cows with chronic mastitis, Eurasian Journal of Veterinary Sciences, Vol. 33, No. 4, 255-259 (2017).Daniel, D.S., Gan, H.M., Lee, S.M., Dykes, G.A., Rahman, S. (2017).Draft Genome Sequences of Six Enterococcus faecalis Strains Isolated from Malaysian Clinical and Environmental Origins,, Genome Announcements, Vol. 5, No. 24. Daniel, D.S., Lee, S.M., Gan, H.M., Dykes, G.A., Rahman, S.(2017) )Genetic diversity of Enterococcus faecalis isolated from environmental, animal and clinical sources in Malaysia, . Journal of Infection and Public Health Vol. 10, No. 5, 617-623.Elmali, M., Can, H.Y. (2017). Occurence and antimicrobial resistance of Arcobacter species in food and slaughterhouse samples, , Journal of Food Science and Technology, Vol. 37, No. 2, 280-285 (2017). Fibriana, F., Amalia, A.V., Mubarok,I. (2017). )Isolation and Characterization of Pigment Generating Microorganisms from Potato Skin. . Jurnal Mipa, Vol. 40, No. 1, 7-13 (2017).Guran, H.S., Vural, A., Erkan, M.E., Durmusoglu. (2017)Prevalence and some virulence genes of E. coli O157 isolated from chicken meats and giblets Ann. Anim. Sci., Vol. 17, No. 2 (2017) 555–563 Idrees, H.A., Omer, A.F.A., Elaas, W.A., Mohammed, Z.M.S., Sidig, M., (2017) Serological Significance of Anti-HBc IgG Marker detected in Sudanese Haemodialysis Patients . American Journal of Research Communication2017, 5(8): 1-8.Wilantho, A., Deekaew, P., Srissutti, C., Tongisma, S., Somboonna, N. (2017). Diversity of bacterial communities on the facial skin of different age-group Thai males PeerJ, (2017).Zakaria, N.H., Tajarudin, H.A., Sapingi, M.S.M., Murshed, M.F. (2017).Identification of Pathogenic Bacteria Isolated from Raw and After Sand Filtration Water at Lubok Buntar Water Treatment Plant, Scientific Research Journal, Vol. 14, No. 1 (2017).Azwai, S.M.et al. (2016) Isolation and Molecular Identification of Vibrio spp. By sequencing of 16S rDNA from seafood, meat and meat products in Libya. Open Veterinary Journal, 6(1), p. 36-43.Aw, Y.K., et al (2016) Newly Isolated Paenibacillus tyrfis sp. nov., from Malaysian Tropical Peat Swamp Soil with Broad Spectrum Antimicrobial Activity. Frontiers in Microbiology. 7(219), p. 1-9.Copur-Cicek,A., Dinc-Patlak, R., Sandalli, C., Mengeloğlu, Z., Kostakoglu, U., Yildiz, I.E. (2016). Determination of the staphylococcal cassette chromosome in methicillin-resistant Staphylococcus aureus strains isolated from various clinical samples. . Russia Open Medical Journal. 5(2). Luang-In,V., Deeseenthum, S. (2016) Exopolysaccharide-producing isolates from Thai milk kefir and their antioxidant activities. . LWT - Food Science and Technology.73. Pp592-601 Merghani, E.A.E., Elsanousi, S.M., Hamedelnil, Y.F., Gornas, N.A. (2016).Molecular Study of Bacterial Pathogens Isolated From Haemodialysis Patients, Sudan Journal of Science and Technology, Vol 12, No. 1, 1-10 (2016).Mohamad, Y., Reda, W.W., Abdel-Moein, K., El-Razil, K.A.A., Barakat, A.M.A., El Fadaly, H.A., Hassanain, N.A., Hegazi, A.G. (2016) Prevalence and phylogenetic characterization of Listeria Monocytogenes isolated from processed meat marketed in Egypt.. Journal of Genetic Engineering and Biotechnology. 14(1). Pp.119-123 Raweekul, W., Wuttitummaporn, S., Sodchuen, W., Kittiwongwattana, C. (2016).Plant Growth Promotion by Endophytic Bacteria Isolated from Rice (Oryza sativa), Thammasat International Journal of Science and Technology,Vol. 21, No. 1 (2016).Supong, K., Thawai, C., Supothina, S., Auncharoen, P., Pittayakhajonwut, P. (2016) ) Antimicrobial and anti-oxidant activities of quinoline alkaloids from Pseudomonas aeruginosa BCC76810. Phytochemistry Letters. 17. Pp.100-106Uttatree,S., Charoenpanich, J. (2016) Isolation and characterization of a broad pH- and temperature-active, solvent and surfactant stable protease from a new strain of Bacillus subtilis. . Biocatalysis and Agricultural Biotechnology. 8. Pp.32-38Abdulrahman, S.M. (2015). Multiplex PCR for direct identification of Campylobacter Species in Human Stool Journal of Biomedical and Pharmaceutical Research, Vol. 4, No. 3 (2015). Akinsaya, M. A. et al. (2015) Metagenomics Study of Endophytic Bacteria in Aloe Vera Using Next Generation Technology. Genomics Data. ScienceDirect6, p. 159-163. Chua, P., Har, Z.M., Austin, C.M., Yule, C.N., Dykes, G.A., Lee, S.M. (2015) Genome sequencing and annotation of Aeromonas sp. HZ. . Genomics Data. 5 pp38-39 Chua, P., Har, Z.M., Austin, C.M., Yule, C.N., Dykes, G.A., Lee, S.M. (2015) Genome sequencing and annotation of Cellulomonas sp. HZM. . Genomics Data.5. Pp40-41Erdogmus, S.F., et al (2015) Aromatic Hydrocarbon Utilization Ability of Chromohalobacter sp. Ekoloji94: 10-16. Khalafalla, A.I., Al-Busada, K.A., & El-Sabagh, I.M. (2015)Multiplex PCR for Rapid Diagnosis and Differentiation of Pox and Pox-like Diseases in Dromedary Camels. Virology Journal. 12(102), p. 1-10.Ntougias, S., Melidis, P., Navrozidou, E., Tzegkas, F. (2015) Diversity and efficiency of anthracene-degrading bacteria isolated from a denitrifying activated sludge system treating municipal wastewater. International Biodeterioration & Biodegradation. 97. Pp..151-158.Abd El-Salam, N.M., Ayaz, S., & Ullah, R. (2014) PCR and Microscopic Identification of Isolated Leishmania tropica from Clinical Samples of Cutaneous Leishmaniasis in Human Population of Kohat Region in Khyber Pakhtunkhwa. BioMed Research International. Abd El-Salam, N.M., Ayaz, S., & Ullah, R. (2014) PCR and Microscopic Identification of Isolated Leishmania tropica from Clinical Samples of Cutaneous Leishmaniasis in Human Population of Kohat Region in Khyber Pakhtunkhwa. BioMed Research International. Chua, P., et al. (2014)Draft Genone Sequences of Two Cellulolytic Paenibacillus sp. Strains, MAEPY1 and MAEPY2, from Malaysian Landfill Leachate. Genone Announcements.2(1). El-Salam et al. (2014) PCR and Microscopic Identification of Isolated Leishmania tropica from Clinical Samples of Cutaneous Leishmaniasis in Human Population of Kohat Region in Khyber Pakhtunkhwa Biomed Res Int92014: 861831. Heng, J.L.S., & Hamzah, H. (2014) Biological Active Compounds from Actinomycetes Isolated from Soil of Langkawi Island, Malaysia. African Journal of Biotechnology.ProQuest. 13(49), p. 4523-4528 Rahama, A.B.M., et. al (2014) Molecular Detection of helicobacter pylori Among Gastroduodenitis and Peptic Ulcer Patients in Khartoum State. Journal of Biomedical and Pharmaceutical Research3(5): 41-44 Ting, A.S.Y., Hermanto, A., Peh, K.L. (2014)Indigenous actinomycetes from empty fruit bunch compost of oil palm: Evaluation on enzymatic and antagonistic properties. Biocatalysis and Agricultural Biotechnology. 3. Pp..310-315.Wanangkarn, A., Liu, D.C., Swetwiwathana, A., Jindaprasert, A., Phraephaisarn, C., Chumnqoen, W., Tan, F.J. (2014) Lactic acid bacterial population dynamics during fermentation and storage of Thai fermented sausage according to restriction fragment length polymorphism analysis. International Journal of Food Microbiology. 186. Pp..61-67Erdomus, S.F., et al. (2013) Aromatic Hydrocarbon Degradation by Halophilic Archaea Isolated from Camalti Saltern, Turkey Water, Air and Soil Pollution. ProQuest. p. 1-9.Hafshejani, M.K., et al (2013)Sequential Microaerophilic-Oxic Phase Mineralization of Azo Dyes by a Monoculture of Pseudomonas Aeruginosa Strain AWF Isolated from Textile Wastewater Water Air and Soil Pollution. ProQuest. p. 1-16.Naher, U.A., Othman, R., Abdul-Latif, M., Panhwar, Q.A., Amaddin, P.A.M., Shamsuddin, Z.H. (2013). Biomolecular Characterization of Diazotrophs Isolated from the Tropical Soil in Malaysia, International Journal of Molecular Sciences, Vol. 14, 17812-17829 (2013).Gumel et al.(2012)Biosynthesis and Characterization of Polyhydroxyalkanoates Copolymers Produced by Pseudomonas putida Bet001 Isolated from Palm Oil Mill Effluent PLoS One7(9).Pongsilp, N., et al (2012) Genotypic Diversity among Rhizospheric Bacteria of Three Legumes Assessed by Cultivation-dependent and Cultivation-independent Techniques World Journal of Microbiology and Biotechnology. ProQuest. 28, p. 615-626.Shamsudin, M.N., et al (2012)High Prevalence of qacA/B Carriage among Clinical Isolates of Meticillin-resistant Staphylococcus aureus in Malaysia Journal of Hospital Infection81: 206Charoenpanich, J., Suktanarag, S., & Toobbucha, N. (2011)Production of Thermostable Lipase by Aeromonas sp. EBB-1 Isolated from Marine Sludge in Angsila, Thailand ScienceAsia37: 105-114.Mat Hussin, N. H. et al (2011)Characterization of Bacteria and Fungi Bioaerosol in the Indoor Air of Selected Primary Schools in Malaysia Indoor and Built Environment. Sage Journals20(6), p. 607-617Atshan, S.S., & Shamsudin, M.N. (2010)Evaluation of Phenotypic and Genotypic Detection Methods for Biofilm-forming Methicillin-sensitive and Methicillin-resistant Staphylococcus aureus Clinical Isolates Annals of Microbiology. Uttatree, S., Winayanuwattikun, P., & Charoenpanich, J. (2010) Isolation and Characterization of a Novel Thermophilic-organic Solvent Stable Lipase from Acinetobacter baylyi Applied Biochemistry and Biotechnology Zakaria, M.R., et al. (2010)Polyhydroxyalkanoate Production from Anaerobically Treated Palm Oil Mill Effluent by New Bacterial Strain Comamonas sp. EB172 World Journal of Microbiology and Biotechnology. ProQuest. 26, p. 767-774.

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1.溶液I—溶菌液:溶菌酶:它是糖苷水解酶,能水解菌体细胞壁的主要化学成分肽聚糖中的β-1,4糖苷键,因而具有溶菌的作用。当溶液中pH小于8时,溶菌酶作用受到抑制。葡萄糖:增加溶液的粘度,维持渗透压,防止DNA受机械剪切力作用而降解。EDTA:(1)螯合Mg2+、Ca2+等金属离子,抑制脱氧核糖核酸 查看更多>
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Z2超保真DNA聚合酶是由杭州谨澳生物科技有限公司代理或销售的GZL品牌的试剂,产品来源于中国。杭州谨澳生物科技有限公司是中国最权威的Z2超保真DNA聚合酶试剂销售服务商之一,在杭州等地方销售Z2超保真DNA聚合酶试剂已经多年。生物在线为您提供众多企业Z2超保真DNA聚合酶仪器产品及图片,以便挑选到性价比高,合适的Z2超保真DNA聚合酶产品 查看更多>
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相同点:都能以DNA为模板,从5'向3'进行核苷酸或脱氧核苷酸的聚合反应。
不同点:
1、作用底物不同。RNA聚合酶底物是NTP;DNA聚合酶底物是dNTP。
2、RNA聚合酶作用不需要引物,而DNA聚合酶作用需要引物。
3、RNA聚合酶本身具有一定的解旋功能,而DNA聚合酶没有,当需要解开双链的时候要解旋酶和拓扑异构酶的帮助。
4、RNA聚合酶只具有5‘到3’端的聚合酶活性,而DNA聚合酶不仅有5‘到3’端的聚合酶活性,还具有3‘到5’端的外切酶活性。保证DNA复制时候校对,所以复制的忠实性高于转录的。
5、RNA聚合酶通常作用于转录过程;DNA聚合酶通常作用于DNA复制过程。
A、DNA转录形成RNA时需要RNA聚合酶的催化,底物是核糖核苷酸,A错误;
B、酶大部分是蛋白质、少量是RNA,故某种酶的基本组成单位是氨基酸或核糖核苷酸,B错误;
C、内分泌细胞能产生激素,活的细胞能产生酶,故能产生激素的细胞就能产生酶,C正确;
D、酶通常是蛋白质,蛋白质在低温条件下更加稳定,利于保存,而且低温不会使酶失活,因此在最适温度保存没有意义,D错误.
相同点:都能以DNA为模板,从5'向3'进行核苷酸或脱氧核苷酸的聚合反应。
不同点
1、作用底物不同。RNA聚合酶底物是NTP;DNA聚合酶底物是dNTP。
2、RNA聚合酶作用不需要引物,而DNA聚合酶作用需要引物。
3、RNA聚合酶本身具有一定的解旋功能,而DNA聚合酶没有,当需要解开双链的时候要解旋酶和拓扑异构酶的帮助。
4、RNA聚合酶只具有5‘到3’端的聚合酶活性,而DNA聚合酶不仅有5‘到3’端的聚合酶活性,还具有3‘到5’端的外切酶活性。保证DNA复制时候校对,所以复制的忠实性高于转录的。
5、RNA聚合酶通常作用于转录过程;DNA聚合酶通常作用于DNA复制过程
最近忽然对我们平常用的DNA聚合酶发生研究兴趣,想问下园子里面有没有谁实验室有那些个hot-start、Pfu之类的DNA聚合酶的重组质粒载体?这些酶的活性或是保真性应该强于我们平时使用的Taq酶,谁要是很幸运的找老外讨到了这些重组的质粒载体,PM我,谈谈交换的条件,呵呵!
DNA连接酶DNA聚合酶作用的异同:DNA聚合酶只能将()加到己有的核苷酸片段末端,形成磷酸二酯键。DNA链接酶是链接()的末端,形成磷酸二酯键。
不是
DNA聚合酶和DNA连接酶作用的位点都是3'5'磷酸二酯键;但DNA连接酶是作用在游离的DNA片段间,使其连接成为一条完整的DNA链,而DNA聚合酶则是将游离的脱氧核糖核苷酸连接成DNA片段。
端粒酶 123
shineck62014-07-18
看到原核生物DNA复制结束时,引物水解后用DNA聚合酶1补充留下的空白链。但是真核生物是水解引物后要通过端粒酶先合成一段后再用DNA聚合酶修补…不太明白为什么真核生物不能直接用DNA聚合酶修补水解引物之后留下的空白呢?端粒酶与DNA聚合酶有什么区别呢?
合理选择耐热DNA聚合酶是PCR成败与否的一个关键因素。选择最合适的耐热聚合酶,是进行PCR实验首先要考虑的问题。许多耐热DNA聚合酶的主要区别在于特异性、保真性、耐热性、扩增速率、扩增片段长度等几个指标。
下面是六种不同耐热聚合酶的比较:
Taq:扩增效率最高的耐热DNA聚合酶,能很好的扩增6kb以下的DNA片段。扩增碱基出错率为10-5左右。
Pfu:目前保真度最高的耐热DNA聚合酶,碱基出错率为10-6,但扩增效率低于Taq酶,一般能很好的扩增2kb以下的片段。
TaqPlus:集扩增效率高和保真度好于一身。扩增效率比Pfu高,保真度比Taq好。能有效的扩增10kb以下的片段。
HotstartTaq:经过化学修饰的耐热DNA聚合酶。此酶在常温下,活性被化学基团封闭,要在94℃-95℃加热数分钟才能回复正常活力开始反应,避免了起始循环较低温度下的非特异性扩增,提高了反应的灵敏度和特异性。
LongTaq:具有3’-5’外切酶活性的耐热DNA聚合酶,它不但扩增效率高而且错配率低,对于简单模板可扩增长达40kb的模板,对复杂模板也可扩增长达15kb的片段。
TaqPlatinum:热启动高保真耐热DNA聚合酶。如果对保真度要求很高,而用Pfu扩增有难度,可选用TaqPlatinum,一般扩增长度可达4kb。
根据不同的实验目的选择最合适的酶,

克隆普通长度的目的DNA片段:Taq、TaqPlus
保真度要求较高,片段比较短,如点突变、基因筛选等:Pfu、TaqPlatinum
高保真长片段扩增,如构建基因图谱及分子遗传学研究等:LongTaq、TaqPlus
扩增基因组模板,需要降低背景:HotstartTaq、TaqPlatinum
扩增GC含量较高或二级结构较复杂的模板:TaqPlus、LongTaq、TaqPlatinum
实时荧光定量PCR反应:Taq、HotstartTaq、TaqPlatinum
从菌株或质粒模板,筛选鉴定目的克隆,扩增6kb以下的片段:Taq、TaqPlus、2×TaqPCRMasterMix
模板比较复杂或目的片段丰度低,用普通Taq酶扩增不出条带:2×TaqPCRMasterMix、2×PfuPCRMasterMix
一般来说,以DNA为模板的DNA聚合酶和RNA聚合酶都的结合位点都在DNA上。
但是也有以RNA为模板的DNA聚合酶和RNA聚合酶 (也就是反转录酶和RNA依赖的RNA聚合酶),它们的结合位点在RNA上.
也就是说,模版是谁,结合位点就在谁上。
2.结合位点就是启动合成DNA或者RNA的碱基序列,如RNA聚合酶结合位点是转录起始位点,是一段特殊的位于编码基因上游的DNA序列.这段DNA序列可以结合RNA聚合酶,从而起始转录过程.经典的RNA聚合酶结合位点是TATA box.
TATA box是编码序列前的4个碱基.在大多数生物的基因前面都有TATA着4个碱基序列的存在,它就是一个RNA聚合酶结合位点.
RT说得概括一点考试简答用~!
TaqDNA聚合酶是Mg2+依赖性酶,该酶的催化活性对Mg2+浓度非常敏感。以活性程度很低的鲑鱼精子DNA为模板,dNTP的浓度为0.7~0.8mmol/L时,用不同浓度Mg2+进行PCR反应10min,测定结果为Mgcl2浓度在2.0mmol/L时该酶催化活性最高,此浓度能最大限度地激活TaqDNA聚合酶的活性,Mg2+过高就抑制酶活性,当Mgcl2浓度在10mmol/L时可抑制40~50%的酶活性。由于Mg2+能与dNTP结合而影响PCR反应液中游离的Mg2+浓度,因而Mgcl2的浓度在不同的反应体系中应适当调整,优化浓度。一般反应中Mg2+浓度至少应比dNTP总浓度高0.5~1.0mmol/L。适当浓度的KCl能使Taq DNA聚合酶的催化活性提高50~60%,其最适浓度为50mmol/L,高于75mmol/L时明显抑制该酶的活性。
我以质粒为模板进行定点突变,质粒加目的片段一共6.5kb,不知道选用那种高保真聚合酶比较好,想选PfuDNA聚合酶,查了查比较好的公司Promega生产的了,现在又查到TaKaRa生产的PyrobestDNA聚合酶,不知道这两种酶哪个比较好一些。请哪位高手指点一下。
DNA聚合酶作用于DNA复制时,用于连接两个脱氧核苷酸之间的磷酸二酯键以及碱基互补配对时的氢键。
DNA连接酶作用于基因工程,用于连接两个DNA片段间的磷酸二酯键。