
DescriptionThe GF-1 Bacterial DNA Extraction Kit provides a rapid and efficient method for purification of high molecular weight genomic DNA from either Gram-negative or Gram-positive bacteria. The purification is based on the usage of denaturing agents to provide lysis of cells, denaturation of proteins and subsequently release of genomic DNA. Special buffers provided in the kit are optimized to enhance the binding of DNA onto a specially-treated glass filter membrane for efficient recovery of highly pure genomic DNA.
Features
- Suitable for both Gram-negative or Gram-positive bacteria
- Yields up to 20μg of DNA
- No organic-based extraction required
- Highly pure genomic DNA ready to use for routine molecular biology applications such as restriction enzyme digestion, PCR, Southern blotting and DNA fingerprinting.
Kit Components
- Buffer R1
- Buffer R2
- Buffer BG
- Wash Buffer (concentrate)
- Elution Buffer
- Proteinase K
Ordering Information
Catalog No | Description | Pack Size |
GF-BA-050 | GF-1 Bacterial DNA Extraction Kit | 50 preps |
GF-BA-100 | GF-1 Bacterial DNA Extraction Kit | 100 preps |
DownloadManual
GF-1 Bacterial DNA Extraction Kit
Stability Test Report
GF-1 Bacterial DNA Extraction Kit
PublicationThis Product Has Been Used In: Ekprasert J et al. (2020) Investigating mechanical properties and biocement application of CaCO3, precipitated by a newly-isolated Lysinibacillus sp. WH using artificial neural networks, Scientific Reports,10:16137.Erkihun A & Kausalya R (2020) MCR-1 Gene Encoded Colistin-Resistant Escherichia coli in Raw Chicken Meat and Bean Sprouts in Malaysia, International Journal of Microbiology, Article ID 8853582.Eshamah HL et al. (2020)Extent of pathogenic and spoilage microorganisms in whole muscle meat, meat products and seafood sold in Libyan market, Open Veterinary Journal,10(3):276-288.Fatin Aina Zulkhairi Amin et al. (2020). Probiotic Properties of Bacillus Strains Isolated from Stingless Bee (Heterotrigona itama) Honey Collected across Malaysia,. Int. J. Environ. Res. Public Health, 17, 278.Nonzee H et al. (2020) Interaction Study of Pasteurella Multocida with Culturable Aerobic Bacteria Isolated from Porcine Respiratory Tracts using Coculture in Conditioned Media, Research Square,https://doi.org/10.21203/rs.3.rs-36993/v1Pongrawee N & Neelawan P (2020)Distribution and expression of virulence genes in potentially pathogenic bacteria isolated from seafood in Thailand, CyTA – Journal of Food 18:1, 753-763.Virun Vichaibun & Panan Kanchanaphum (2020) Quantitative LAMP and PCR Detection of Salmonella in Chicken Samples Collected from Local Markets around Pathum Thani Province, Thailand, International Journal of Food Science, Article ID 8833173.in vitro antimicrobial assessment on lactic acid bacteria isolated from common freshwater fishes,. Asia-Pacific Journal of Molecular Biology and Biotechnology, 27(2):18-25.Mankai H et al (2019) Complete Genome Sequence and Methylome Analysis of Thermoactinomyces vulgaris 2H, . Microbiology Resource Announcements, 8:e00657-19.Tung HJ et al. (2019)cDNA-SSR Markers for Molecular Epidemiology of Ganoderma boninense,. , Journal of Oil Palm Research, 31(2):220-237. Boonyayatra, S., Tharavichitkut, P., Oliver, S.P. (2018). Virulence-associated genes and molecular typing of Streptococcus uberis associated with bovine mastitis in northern Thailand, Turkish Journal of Veterinary and Animal Sciences, Vol. 42, 73-81 (2018).Community structures of total bacterial DNA, cultivable bacteria and prototrophs in bulk soil and rhizospheres . Can, H.Y., Elmali, M., Ergün, Y. (2017).Methicillin-resistant Staphylococcus aureus in milk from dairy cows with chronic mastitis, Eurasian Journal of Veterinary Sciences, Vol. 33, No. 4, 255-259 (2017).Daniel, D.S., Gan, H.M., Lee, S.M., Dykes, G.A., Rahman, S. (2017).Draft Genome Sequences of Six Enterococcus faecalis Strains Isolated from Malaysian Clinical and Environmental Origins,, Genome Announcements, Vol. 5, No. 24. Daniel, D.S., Lee, S.M., Gan, H.M., Dykes, G.A., Rahman, S.(2017) )Genetic diversity of Enterococcus faecalis isolated from environmental, animal and clinical sources in Malaysia, . Journal of Infection and Public Health Vol. 10, No. 5, 617-623.Elmali, M., Can, H.Y. (2017). Occurence and antimicrobial resistance of Arcobacter species in food and slaughterhouse samples, , Journal of Food Science and Technology, Vol. 37, No. 2, 280-285 (2017). Fibriana, F., Amalia, A.V., Mubarok,I. (2017). )Isolation and Characterization of Pigment Generating Microorganisms from Potato Skin. . Jurnal Mipa, Vol. 40, No. 1, 7-13 (2017).Guran, H.S., Vural, A., Erkan, M.E., Durmusoglu. (2017)Prevalence and some virulence genes of E. coli O157 isolated from chicken meats and giblets Ann. Anim. Sci., Vol. 17, No. 2 (2017) 555–563 Idrees, H.A., Omer, A.F.A., Elaas, W.A., Mohammed, Z.M.S., Sidig, M., (2017) Serological Significance of Anti-HBc IgG Marker detected in Sudanese Haemodialysis Patients . American Journal of Research Communication2017, 5(8): 1-8.Wilantho, A., Deekaew, P., Srissutti, C., Tongisma, S., Somboonna, N. (2017). Diversity of bacterial communities on the facial skin of different age-group Thai males PeerJ, (2017).Zakaria, N.H., Tajarudin, H.A., Sapingi, M.S.M., Murshed, M.F. (2017).Identification of Pathogenic Bacteria Isolated from Raw and After Sand Filtration Water at Lubok Buntar Water Treatment Plant, Scientific Research Journal, Vol. 14, No. 1 (2017).Azwai, S.M.et al. (2016) Isolation and Molecular Identification of Vibrio spp. By sequencing of 16S rDNA from seafood, meat and meat products in Libya. Open Veterinary Journal, 6(1), p. 36-43.Aw, Y.K., et al (2016) Newly Isolated Paenibacillus tyrfis sp. nov., from Malaysian Tropical Peat Swamp Soil with Broad Spectrum Antimicrobial Activity. Frontiers in Microbiology. 7(219), p. 1-9.Copur-Cicek,A., Dinc-Patlak, R., Sandalli, C., Mengeloğlu, Z., Kostakoglu, U., Yildiz, I.E. (2016). Determination of the staphylococcal cassette chromosome in methicillin-resistant Staphylococcus aureus strains isolated from various clinical samples. . Russia Open Medical Journal. 5(2). Luang-In,V., Deeseenthum, S. (2016) Exopolysaccharide-producing isolates from Thai milk kefir and their antioxidant activities. . LWT - Food Science and Technology.73. Pp592-601 Merghani, E.A.E., Elsanousi, S.M., Hamedelnil, Y.F., Gornas, N.A. (2016).Molecular Study of Bacterial Pathogens Isolated From Haemodialysis Patients, Sudan Journal of Science and Technology, Vol 12, No. 1, 1-10 (2016).Mohamad, Y., Reda, W.W., Abdel-Moein, K., El-Razil, K.A.A., Barakat, A.M.A., El Fadaly, H.A., Hassanain, N.A., Hegazi, A.G. (2016) Prevalence and phylogenetic characterization of Listeria Monocytogenes isolated from processed meat marketed in Egypt.. Journal of Genetic Engineering and Biotechnology. 14(1). Pp.119-123 Raweekul, W., Wuttitummaporn, S., Sodchuen, W., Kittiwongwattana, C. (2016).Plant Growth Promotion by Endophytic Bacteria Isolated from Rice (Oryza sativa), Thammasat International Journal of Science and Technology,Vol. 21, No. 1 (2016).Supong, K., Thawai, C., Supothina, S., Auncharoen, P., Pittayakhajonwut, P. (2016) ) Antimicrobial and anti-oxidant activities of quinoline alkaloids from Pseudomonas aeruginosa BCC76810. Phytochemistry Letters. 17. Pp.100-106Uttatree,S., Charoenpanich, J. (2016) Isolation and characterization of a broad pH- and temperature-active, solvent and surfactant stable protease from a new strain of Bacillus subtilis. . Biocatalysis and Agricultural Biotechnology. 8. Pp.32-38Abdulrahman, S.M. (2015). Multiplex PCR for direct identification of Campylobacter Species in Human Stool Journal of Biomedical and Pharmaceutical Research, Vol. 4, No. 3 (2015). Akinsaya, M. A. et al. (2015) Metagenomics Study of Endophytic Bacteria in Aloe Vera Using Next Generation Technology. Genomics Data. ScienceDirect6, p. 159-163. Chua, P., Har, Z.M., Austin, C.M., Yule, C.N., Dykes, G.A., Lee, S.M. (2015) Genome sequencing and annotation of Aeromonas sp. HZ. . Genomics Data. 5 pp38-39 Chua, P., Har, Z.M., Austin, C.M., Yule, C.N., Dykes, G.A., Lee, S.M. (2015) Genome sequencing and annotation of Cellulomonas sp. HZM. . Genomics Data.5. Pp40-41Erdogmus, S.F., et al (2015) Aromatic Hydrocarbon Utilization Ability of Chromohalobacter sp. Ekoloji94: 10-16. Khalafalla, A.I., Al-Busada, K.A., & El-Sabagh, I.M. (2015)Multiplex PCR for Rapid Diagnosis and Differentiation of Pox and Pox-like Diseases in Dromedary Camels. Virology Journal. 12(102), p. 1-10.Ntougias, S., Melidis, P., Navrozidou, E., Tzegkas, F. (2015) Diversity and efficiency of anthracene-degrading bacteria isolated from a denitrifying activated sludge system treating municipal wastewater. International Biodeterioration & Biodegradation. 97. Pp..151-158.Abd El-Salam, N.M., Ayaz, S., & Ullah, R. (2014) PCR and Microscopic Identification of Isolated Leishmania tropica from Clinical Samples of Cutaneous Leishmaniasis in Human Population of Kohat Region in Khyber Pakhtunkhwa. BioMed Research International. Abd El-Salam, N.M., Ayaz, S., & Ullah, R. (2014) PCR and Microscopic Identification of Isolated Leishmania tropica from Clinical Samples of Cutaneous Leishmaniasis in Human Population of Kohat Region in Khyber Pakhtunkhwa. BioMed Research International. Chua, P., et al. (2014)Draft Genone Sequences of Two Cellulolytic Paenibacillus sp. Strains, MAEPY1 and MAEPY2, from Malaysian Landfill Leachate. Genone Announcements.2(1). El-Salam et al. (2014) PCR and Microscopic Identification of Isolated Leishmania tropica from Clinical Samples of Cutaneous Leishmaniasis in Human Population of Kohat Region in Khyber Pakhtunkhwa Biomed Res Int92014: 861831. Heng, J.L.S., & Hamzah, H. (2014) Biological Active Compounds from Actinomycetes Isolated from Soil of Langkawi Island, Malaysia. African Journal of Biotechnology.ProQuest. 13(49), p. 4523-4528 Rahama, A.B.M., et. al (2014) Molecular Detection of helicobacter pylori Among Gastroduodenitis and Peptic Ulcer Patients in Khartoum State. Journal of Biomedical and Pharmaceutical Research3(5): 41-44 Ting, A.S.Y., Hermanto, A., Peh, K.L. (2014)Indigenous actinomycetes from empty fruit bunch compost of oil palm: Evaluation on enzymatic and antagonistic properties. Biocatalysis and Agricultural Biotechnology. 3. Pp..310-315.Wanangkarn, A., Liu, D.C., Swetwiwathana, A., Jindaprasert, A., Phraephaisarn, C., Chumnqoen, W., Tan, F.J. (2014) Lactic acid bacterial population dynamics during fermentation and storage of Thai fermented sausage according to restriction fragment length polymorphism analysis. International Journal of Food Microbiology. 186. Pp..61-67Erdomus, S.F., et al. (2013) Aromatic Hydrocarbon Degradation by Halophilic Archaea Isolated from Camalti Saltern, Turkey Water, Air and Soil Pollution. ProQuest. p. 1-9.Hafshejani, M.K., et al (2013)Sequential Microaerophilic-Oxic Phase Mineralization of Azo Dyes by a Monoculture of Pseudomonas Aeruginosa Strain AWF Isolated from Textile Wastewater Water Air and Soil Pollution. ProQuest. p. 1-16.Naher, U.A., Othman, R., Abdul-Latif, M., Panhwar, Q.A., Amaddin, P.A.M., Shamsuddin, Z.H. (2013). Biomolecular Characterization of Diazotrophs Isolated from the Tropical Soil in Malaysia, International Journal of Molecular Sciences, Vol. 14, 17812-17829 (2013).Gumel et al.(2012)Biosynthesis and Characterization of Polyhydroxyalkanoates Copolymers Produced by Pseudomonas putida Bet001 Isolated from Palm Oil Mill Effluent PLoS One7(9).Pongsilp, N., et al (2012) Genotypic Diversity among Rhizospheric Bacteria of Three Legumes Assessed by Cultivation-dependent and Cultivation-independent Techniques World Journal of Microbiology and Biotechnology. ProQuest. 28, p. 615-626.Shamsudin, M.N., et al (2012)High Prevalence of qacA/B Carriage among Clinical Isolates of Meticillin-resistant Staphylococcus aureus in Malaysia Journal of Hospital Infection81: 206Charoenpanich, J., Suktanarag, S., & Toobbucha, N. (2011)Production of Thermostable Lipase by Aeromonas sp. EBB-1 Isolated from Marine Sludge in Angsila, Thailand ScienceAsia37: 105-114.Mat Hussin, N. H. et al (2011)Characterization of Bacteria and Fungi Bioaerosol in the Indoor Air of Selected Primary Schools in Malaysia Indoor and Built Environment. Sage Journals20(6), p. 607-617Atshan, S.S., & Shamsudin, M.N. (2010)Evaluation of Phenotypic and Genotypic Detection Methods for Biofilm-forming Methicillin-sensitive and Methicillin-resistant Staphylococcus aureus Clinical Isolates Annals of Microbiology. Uttatree, S., Winayanuwattikun, P., & Charoenpanich, J. 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DNA聚合酶和DNA连接酶作用的位点都是3'5'磷酸二酯键;但DNA连接酶是作用在游离的DNA片段间,使其连接成为一条完整的DNA链,而DNA聚合酶则是将游离的脱氧核糖核苷酸连接成DNA片段。
不同点:
1、作用底物不同。RNA聚合酶底物是NTP;DNA聚合酶底物是dNTP。
2、RNA聚合酶作用不需要引物,而DNA聚合酶作用需要引物。
3、RNA聚合酶本身具有一定的解旋功能,而DNA聚合酶没有,当需要解开双链的时候要解旋酶和拓扑异构酶的帮助。
4、RNA聚合酶只具有5‘到3’端的聚合酶活性,而DNA聚合酶不仅有5‘到3’端的聚合酶活性,还具有3‘到5’端的外切酶活性。保证DNA复制时候校对,所以复制的忠实性高于转录的。
5、RNA聚合酶通常作用于转录过程;DNA聚合酶通常作用于DNA复制过程。
DNA连接酶作用于基因工程,用于连接两个DNA片段间的磷酸二酯键。
最近,我看了很多这方面的资料,但是我对这个方面不是很了解,所以现在搞得头昏眼花的,不知道该选什么酶和载体,希望大家多多帮忙
在此十分感谢
可分为以下几个类群:(1)依赖DNA的DNA聚合酶;(2)依赖RNA的DNA聚合酶;(3)依赖DNA的RNA聚合酶;(4)依赖RNA的RNA聚合酶。前两者是DNA聚合酶,它使DNA复制链按模板顺序延长。如在原核生物中仅就大肠杆菌中已被发现的就有三种(分别简称为PolⅠ,PolⅡ和PolⅢ等);DNA聚合酶只能在有引物的基础上,即在DNA或RNA引物的3′-OH延伸,这DNA的合成方向记为5′→3′。换言之DNA聚合酶催化反应除底物(αNTP)外,还需要Mg2+ 、模板DNA和引物,迄今细胞内尚无发现可从单体起始DNA的合成。同样,上述(3)和(4)是催化RNA生物合成反应中最主要的RNA合成酶,它们以四种三磷酸核糖核苷(NTP)为底物,并需有DNA模板以及Mn2 及Mg2 的存在下,在前一个核苷酸3′-OH与下一个核苷酸的5′-P聚合形成3′,5′-磷酸二酯键,其新生链的方向也是5′→3′。RNA聚合酶也大量存在于原核和真核生物的细胞中。如大肠杆菌RNA聚合酶分子量4.8×105,由5条多肽链组成,分别命名为α,α,β,β′,和γ,全酶可用α2ββ′λ表示。真核生物RNA聚合酶分子大于5×105,由10~12个大小不等亚基组成。聚合酶除作为自然界生命活动中不可缺少的组分外,在实验室中大多用作生命科学研究的工具酶类之一。
B、酶大部分是蛋白质、少量是RNA,故某种酶的基本组成单位是氨基酸或核糖核苷酸,B错误;
C、内分泌细胞能产生激素,活的细胞能产生酶,故能产生激素的细胞就能产生酶,C正确;
D、酶通常是蛋白质,蛋白质在低温条件下更加稳定,利于保存,而且低温不会使酶失活,因此在最适温度保存没有意义,D错误.
相同点:都能以DNA为模板,从5'向3'进行核苷酸或脱氧核苷酸的聚合反应。
不同点
1、作用底物不同。RNA聚合酶底物是NTP;DNA聚合酶底物是dNTP。
2、RNA聚合酶作用不需要引物,而DNA聚合酶作用需要引物。
3、RNA聚合酶本身具有一定的解旋功能,而DNA聚合酶没有,当需要解开双链的时候要解旋酶和拓扑异构酶的帮助。
4、RNA聚合酶只具有5‘到3’端的聚合酶活性,而DNA聚合酶不仅有5‘到3’端的聚合酶活性,还具有3‘到5’端的外切酶活性。保证DNA复制时候校对,所以复制的忠实性高于转录的。
5、RNA聚合酶通常作用于转录过程;DNA聚合酶通常作用于DNA复制过程
但是也有以RNA为模板的DNA聚合酶和RNA聚合酶 (也就是反转录酶和RNA依赖的RNA聚合酶),它们的结合位点在RNA上.
也就是说,模版是谁,结合位点就在谁上。
2.结合位点就是启动合成DNA或者RNA的碱基序列,如RNA聚合酶结合位点是转录起始位点,是一段特殊的位于编码基因上游的DNA序列.这段DNA序列可以结合RNA聚合酶,从而起始转录过程.经典的RNA聚合酶结合位点是TATA box.
TATA box是编码序列前的4个碱基.在大多数生物的基因前面都有TATA着4个碱基序列的存在,它就是一个RNA聚合酶结合位点.
聚合酶就是多功能的dna的制作机器

