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Interferon alpha plays a role in viral infections. Signaling takes place through an IFN Recpetor complex consisting of two alpha chains (Type I receptor) compl
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Staining protocols for placental explant culturesWhole mount staining.The protocol given uses a single primary antibody with nuclear counterstaining, but it ca
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From early post-implantation stages to approximately 18 weeks" gestation, cytotrophoblast at the tips of anchoring villi at the placental periphery proliferate
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Orcinol Determination of RNALEVEL I Materials RNAAlkaline distilled waterAcid-orcinol reagentBoiling water bathSpectrophotometer and cuvettes Procedure Prepare
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1. Collected animals can be stored as frozen pellets at -70 degrees. Alternatively, freshly collected animals can be quick frozen in liquid nitrogen. Prechill
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Introduction:C. elegans RNA preps that have been widely used previously involved fairly slow lysis of the worms, potentially leading to degradation of the RNA.
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