Description
C1 Inhibitor Polyclonal Antibody
Affinity’s C1 Inhibitor Polyclonal Antibody is the base level of our C1 Inhibitor antibody family. The purity of IgG is typically 90% and is provided in a solution of HEPES buffered saline containing 50% glycerol (v/v). The titre is essentially the same as the starting antiserum and each vial typically contains the amount of IgG recovered from one milliliter of antiserum. This C1 Inhibitor Polyclonal Antibody is generally intended for use in applications such as immuno-precipitation, immuno-electrophoresis, immuno-depletion and activity neutralization assays.
Product Code: GACINH-IG
Retail Product Size: 5mg vial
Host Animal: Goat Anti-Human C1 Inhibitor Polyclonal Antibody
Species Cross Reactivity: View Chart
Product Datasheet: C1-Esterase Inhibitor Polyclonal Antibody, purified anti-human Goat IgG
C1 Inhibitor Description
C1 Inhibitor (C1-INH), also known as C1 Esterase Inhibitor, is a part of the serpin family of proteases – alpha-1-antitrypsin (A1AT) and antithrombin (ATIII). These proteins together inactivate target proteases and produce complexes with the protein that is to be inhibited. Cl Inhibitor is the major plasma inhibitor of activated Hageman factor (Factor XII) and kallikrein¹. C1-INH also blocks activation of C1 and the classic complement pathway, without C1-INH the activation of C1, C2, and C4 occur before other inhibitors like C4b-binding protein (C4BP) and Complement Factor I or C3b/C4b inactivator can halt the cascade.
References and Reviews
- Kaplan AP, Ghebrehiwet B, Silverberg M, Sealey JE; The intrinsic coagulation-kinin pathway, complement cascades, plasma renin-angiotensin system, and their interrelationships; Crit Rev Immunol 1981 Sep;3(1):75-93.
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DNA连接酶主要是连接DNA片段之间的磷酸二酯键最初从原核生物（大肠杆菌）分离得到的.现在生物基因工程主要是从T4噬菌体中分离得到的,
菌体构建时，目的基因连接在T载体上，测序也正确，但是将目的基因还有载体分别双酶切后总是连接不上，将连接后产物跑核酸胶，什么也没有，不知道是什么原因？我的目的基因浓度为9ng/ul,载体回收后的浓度为6ng/ul,是因为浓度太低的原因吗？还有就是连接有目的基因的T载体双酶切后出现了三条带，这个又是什么原因呢？希望得到解答
连接酶通常是包括“连接酶”这个字，就如DNA连接酶是将脱氧核糖核酸（DNA）片段连接。其他普遍的名称包括“合成酶”，因为这些酶是用作合成新的分子，或当它们是将二氧化碳加入一个分子时则称为“羧化酶”。
大家有用过Invitrogen的T4连接酶吗？说明书上是23－26度连接，一般的连接酶不都是16度吗？应该用多少度呢？另外，说明书上还说连接后为了达到更好的转化效率，应将连接反应液至少稀释5倍再转化，是这样吗？谢谢大家帮忙啊
只不过限制性核苷酸酶是将磷酸二酯键切断；而DNA连接酶则是形成磷酸二酯键。
求有经验的大神指教，我的载体和目的基因连不上，转化不到大肠中，比例为1:3。另外，为啥胶回收后的载体浓度那么低，大约6ng/ul了，影响连接么？
