DescriptionAvian Myeloblastosis Virus (AMV) Reverse Transcriptase is an RNA-dependent DNA polymerase (aß holoenzyme) with molecular weight of 157 kDa. It synthesizes a complementary DNA strand initiating from a primer using either RNA (cDNA synthesis) or single-stranded DNA as a template.
Concentration20u/μl
Assay Condition 50mM Tris- HCl (pH8.3), 6mM MgCl2, 40mM KCl, 4mM DTT, 0.5mM [ 3H]-TTP (10-20c/m/pmol), 0.4mM Poly(rA)•(dT)12-18, in a reaction volume of 25μl.
Supplied With10X Buffer AMV-RT 250mM Tris-HCl (pH 8.3), 500mM KCl, 50mM MgCl2 and 20mM DTT. Store at -70˚C.
Storage Buffer200mM Potassium Phosphate (pH 7.2), 0.2% TritonTM X-100, 2mM DTT and 50% glycerol. Store at -70˚C for long term.Store at -20˚C for short term (1 month)..
Thermal Inactivation 80˚C for 10 minutes.
Unit Definition 1u is defined as the amount of enzyme that is required to incorporate 1nmol of dMTP into an acid-insoluble material in 10 minutes at 37˚C using Poly(rA)•(dT)12-18 as a template primer.
Features
- First strand synthesis of cDNA.
- Synthesis of cDNA for cloning.
- cDNA labelling.
- Primer extensions and RNA sequencing
- RT-PCR
- Dideoxy sequencing of DNA and RNA.
Quality Control All preparations are assayed for contaminating endonuclease, exonuclease and non-specific RNase activities.
Ordering Information
| Catalog No | Description | Pack Size |
|---|---|---|
| ME2301 | AMV Reverse Transcriptase (Recombinant) | 500u |
| ME2302 | AMV Reverse Transcriptase (Recombinant) | 2500u |
DownloadManual
AMV Reverse Transcriptase (Recombinant)
PublicationThis Product Has Been Used In:Lim et al. (2012) Co-administration of Avian Influenza Virus H5 Plasmid DNA with Chicken IL-15 and IL-18 Enhanced Chickens Immune Responses. BMC Vet Res8: 132.
ebiomall.com
>
>
>
>
>
>
>
>
>
>
>
>
连接酶通常是包括“连接酶”这个字，就如DNA连接酶是将脱氧核糖核酸（DNA）片段连接。其他普遍的名称包括“合成酶”，因为这些酶是用作合成新的分子，或当它们是将二氧化碳加入一个分子时则称为“羧化酶”。
DNA连接酶主要是连接DNA片段之间的磷酸二酯键最初从原核生物（大肠杆菌）分离得到的.现在生物基因工程主要是从T4噬菌体中分离得到的,
求有经验的大神指教，我的载体和目的基因连不上，转化不到大肠中，比例为1:3。另外，为啥胶回收后的载体浓度那么低，大约6ng/ul了，影响连接么？
DNA连接酶:可以连接被限制酶切割开磷酸二酯键
菌体构建时，目的基因连接在T载体上，测序也正确，但是将目的基因还有载体分别双酶切后总是连接不上，将连接后产物跑核酸胶，什么也没有，不知道是什么原因？我的目的基因浓度为9ng/ul,载体回收后的浓度为6ng/ul,是因为浓度太低的原因吗？还有就是连接有目的基因的T载体双酶切后出现了三条带，这个又是什么原因呢？希望得到解答
