
Product information | Key steps | References | Technical support | Reviews |
Product overview
Product name | HomoArginine ELISA kit |
Description | Competitive ELISA kit for the quantitative measurement of Homoarginine (hARG) in serum and plasma samples. For research use only. |
Format | 96-well plate |
Samples | Serum, Plasma |
Minimal sample volume | 20 µL |
Species reactivity | React with all species |
Standard range | 0.32 - 12.5 µM |
Sensitivity | 125 nM |
Specificity | No significant cross-reactivity was observed with L-Arginine, Agmatine, L-Citruline, L-Ornithine, ADMA and SDMA. |
Assay time | Sample preparation 3h and ELISA overnight |
Storage | Store at 2-8°C for up to 6 months. |
Datasheets | Instructions for use, Material Safety Datasheet |
Key steps
Sample collection & storage | Serum: Do not use lipemic, haemolytic samples, as well as samples containing precipitates or fibrin strands. Store samples at 2-8°C for up to 48h or -20°C for longer period (up to 6 months). |
Sample preparation | Sample preparation (3 hours) |
ELISA | Homoarginine antiserum overnight incubation, revelation and read steps (1h). |
Detailed protocol | Download instructions for use |
References
Selected articles on Homoarginine:
- Karetnikova et al. Is Homoarginine a Protective Cardiovascular Risk Factor?Arterioscler Thromb Vasc Biol. 2019
- Wetzel et al. L-Homoarginine supplementation prevents diabetic kidney damage.Physiol Rep. 2019
- Helm et al. Plasma Homoarginine Concentrations According to Use of Hormonal Contraception.Sci Rep. 2018
- Atzler et al. L-homoarginine and cardiovascular disease. Curr Opin Clin Nutr Metab Care. 2015
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同一管dna有的酶可以切开,比如dra1,ecoR1,Hind111
有的酶又完全不能切动,如BamH1,sac1,xba1,xho1,pst1
请问高手这是什么原因??
感谢赐教~!
我做的双酶切反应,酶分别是宝生物的BamHI和XhoI,双酶切体系是:载体及目的片段分别是30ul,通用buffer4ul,XhoI、BamHI各2ul,无核酶水2ul,总体积40ul。载体和目的片段都是经37度酶切6h。载体上面这两个酶切位点的距离是6个碱基,目的片段是由pcr反应获得的,从puc19中p出来的,两端带有这两种酶的酶切位点,酶切完成后,做连接反应,体系如下:目的片段(全长1441bp)4ul,无核酶水3ul,10*T4DNA连接酶缓冲液1ul,载体1ul,T4DNA连接酶1ul,总体积10ul。16度过夜。之后摇菌送沉菌测序结果回来,送了5管一个都不对。重复实验两次还是不对!
之后改为先用BanHI单切载体,体系如下:载体17ul,酶1ul,buffer2ul,总体积20ul,30度切6小时后,Promega纯化试剂盒直接纯化,完了之后再用XhoI,37度酶切6小时,体系如上,再次用promega纯化试剂盒纯化,目的片段用双酶切体系酶切,之后做连接反应,之后铺板,挑取菌落共10个,摇菌13小时,取菌液做pcr鉴定,结果10个菌落全是引物2聚体,跑出来的条带都是100bp左右。
各位老师这是怎么回事呢?为什么连接不上呢?小弟先谢过各位大哥了!
求问酶切体系为10ul,内切酶1ul,37℃水浴45min,不知道DNA产物是否完全被切开了?
所用内切酶信息如下
配后是否应该分装,因为不能反复冻融?
多谢!
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【】可以这样输入,智能ABC,输入“v1”,后翻3页,就可以看见了,或用紫光拼音中文输入法,输入方括号就是粗体的中括号,实在不行请复制本公告中【】

