Human Topoisomerase II alpha and beta

Human topoisomerase II alpha is prepared by overexpressing in baculovirus-infected insect cells (Spodoptera frugiperda) and purifying it by methods developed in-house.

The enzyme is supplied in Dilution Buffer.

Store at -80°C.

It is recommended that larger pack sizes (500U and above) of the enzyme are aliquoted to avoid repeated freeze-thaw cycles.

See technical documents below for more detailed information and lot specific activities.

Technical Documents

Cat No.	Product	Price	Quantity
HT201	Human Topo II alpha100 U	£75
HT205	Human Topo II alpha500 U	£230
HT210	Human Topo II alpha1000 U	£385
HT220	Human Topo II alpha2000 U	£640

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Human topoisomerase II beta is prepared by overexpressing in baculovirus-infected insect cells (Spodoptera frugiperda) and purifying it by methods developed in-house.

The enzyme is supplied at a concentration of 2-10 U/μl in Dilution Buffer.

Store at -80°C. (Stable for 3 months undiluted).

It is recommended that the enzyme is aliquoted to avoid repeated freeze-thaw cycles.

Technical Documents

Cat No.	Product	Price	Quantity
HTB201	Human Topo II beta100U	£75
HTB205	Human Topo II beta500U	£230
HTB210	Human Topo II beta1000U	£385
HTB220	Human Topo II beta2000U	£640

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These contain human topo II and the catenated kDNA substrate in addition to the Assay and Dilution buffers for decatenation reactions. 1 U of human topo II will decatenate 200 ng of kDNA when incubated in 1X Assay buffer in a total reaction volume of 30 µl at 37°C for 30 minutes. The kits are available with either the alpha or beta forms of the enzyme. 

Technical Documents

Cat No.	Product	Price	Quantity
HTK201	Human Topo II alpha Decatenation Assay Kit 1Contains 100 U of human topo II alpha and 20 µg kDNA	£195
HTK202	Human Topo II alpha Decatenation Assay Kit 2Contains 500 U of human topo II alpha and 100 µg kDNA	£615
HTK203	Human Topo II alpha Decatenation Assay Kit 3Contains 1000 U of human topo II alpha and 200 µg kDNA	£970
HTK204	Human Topo II alpha Decatenation Assay Kit 4Contains 2000 U of human topo II alpha and 400 µg kDNA	£1,540
HTKB201	Human Topo II beta Decatenation Assay Kit 1Contains 100 U of human topo II beta and 20 µg kDNA	£195
HTKB202	Human Topo II beta Decatenation Assay Kit 2Contains 500 U of human topo II beta and 100 µg kDNA	£615
HTKB203	Human Topo II beta Decatenation Assay Kit 3Contains 1000 U of human topo II beta and 200 µg kDNA	£970
HTKB204	Human Topo II beta Decatenation Assay Kit 4Contains 2000 U of human topo II beta and 400 µg kDNA	£1,540

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These contain human topo II and the supercoiled DNA substrate in addition to the Assay and Dilution buffers for relaxation reactions. 1 U of human topo II will relax 0.5 µg supercoiled pBR322 DNA in 30 minutes at 37°C.  The kits are available with either the alpha or beta forms of the enzyme. 

Technical Documents

Cat No.	Product	Price	Quantity
HTR201	Human Topo II alpha Relaxation Assay Kit 1Contains 100 U of human topo II alpha and 50 µg supercoiled DNA	£195
HTR202	Human Topo II alpha Relaxation Assay Kit 2Contains 500 U of human topo II alpha and 250 µg supercoiled DNA	£615
HTR203	Human Topo II alpha Relaxation Assay Kit 3Contains 1000 U of human topo II alpha and 500 µg supercoiled DNA	£1,150
HTR204	Human Topo II alpha Relaxation Assay Kit 4Contains 2000 U of human topo II alpha and 1 mg supercoiled DNA	£1,800
HTRB201	Human Topo II beta Relaxation Assay Kit 1Contains 100 U of human topo II beta and 50ug supercoiled DNA	£195
HTRB202	Human Topo II beta Relaxation Assay Kit 2Contains 500 U of human topo II beta and 250 µg supercoiled DNA	£615
HTRB203	Human Topo II beta Relaxation Assay Kit 3Contains 1000 U of human topo II beta and 500 µg supercoiled DNA	£1,150
HTRB204	Human Topo II beta Relaxation Assay Kit 4Contains 2000 U of human topo II beta and 1 mg supercoiled DNA	£1,800

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These kits are designed for assaying cell extracts and partially purified fractions containing human topo II. They contain kDNA substrate, Assay buffer, Dilution buffer, decatenated and linear DNA markers and stop buffer / loading dye.

The kit components are based on the standard assay which contains 500 ng substrate DNA per assay.

Technical Documents

Cat No.	Product	Price	Quantity
HDAK1	Kit 1Contains sufficient kDNA, assay and dilution buffers for 100 assays as well as linear and decatenated marker DNA	£164
HDAK2	Kit 2Contains linear and decatenated marker DNA	£410
HDAK3	Kit 3Contains sufficient kDNA, assay and dilution buffers for 500 assays as well as linear and decatenated marker DNA	£525

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The kit is supplied with sufficient human topo II enzyme, plasmid DNA substrate, buffers and other assay components* for 100 assays. The enzyme is supplied at a concentration of 10 U/μl in Dilution Buffer. The kit is also supplied with sufficient wash buffers for one 96-well plate. These buffers are supplied as 20X concentrates and must be diluted with ultra pure water prior to use.  The kits are available with either the alpha or beta forms of the enzyme. 

More information about this assay can be found on the "Services" page under "High/Medium Throughput Assay".

Kit issued with limited licence for individual use only. 

Patent held by Inspiralis Ltd., Norwich, Norfolk, UK. (Patent No. GB0424953.8, US7838230)

Technical Documents

Cat No.	Product	Price	Quantity
TRT201	HTS Assay Kit - Human Topo II 1-5 KitsFor 100 assays per plate	£430
TRT202	HTS Assay Kit - Human Topo II 6+ KitsFor 100 assays per plate	£346
TRTB201	HTS Assay Kit - Human Topo II Beta 1-5 KitsFor 100 assays per plate	£430
TRTB202	HTS Assay Kit - Human Topo II Beta 6+ KitsFor 100 assays per plate	£346

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Human topoisomerase II alpha amino acids 1-453. This contains the ATPase domain, the activity of which can be stimulated by DNA (see Campbell and Maxwell (2002), J.Biol.Chem. 320 p.171.  The protein is expressed in E.coli.

Technical Documents

Cat No.	Product	Price	Quantity
HT453	Human Topo II 453 domainPlease enquire	POA

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