
Description:
pOET1and2arebaculovirustransferplasmidsdesignedforhighlevelexpressionofforeigngenesunderthepowerfulAcMNPVpolyhedrin(polh)promoter.TheyhaveaColE1originofreplicationandanampicillinresistancegeneforselectioninE.coli.Thevectorsaresmallerthanotheravailabletransfervectors(4541bp)whichgreatlyfacilitatesthecloningsteps.Thepolhcodingsequenceshavebeenreplacedbyamultiplecloningsitecontaining14uniquerestrictionsitesforinsertionoftheforeigngeneinthecorrectorientation.pOET2hasthesameMCSaspOET1,butinthereverseorientation.ThePacIsiteattheendoftheMCSprovidestranslationalstopcodonsinallthreereADIngframesforexpressionoftruncatedproteins.TheAcMNPVsequencesflankingtheMCSfacilitaterecombinationwiththevirusDNAtoinserttheexpressioncassetteintothepolhlocus.AswellasourownflashBACplatformthesevectorscanalsobeusedwithanyotherbaculovirussystemthatuseshomologousrecombinationtoinserttheforeigngeneintothevirusgenome.
AdditionalInformation:
Name | pOET-2transferplasmid(10ug) |
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RelatedProductNames | pOET-2transferplasmid(10ug) |
IntendedUse | ResearchUseOnly |