Recombinant Human His6-USP13 Protein, CF Summary
Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human USP13 concentration of 100-500 nM when using Ubiquitin-AMC or Ubiquitin-Rh110 substrates (U-550, U-555)
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins.Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration.The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard.In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
E-588
| Formulation | X mg/ml (X μM) in 50 mM HEPES pH 7.5, 100 mM NaCl, 10% (v/v) Glycerol, 1 mM TCEP |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Reconstitution Calculator
Background: USP13
Ubiquitin carboxyl-terminal hydrolase 13 (USP13) is a specialized cysteine protease with a predicted molecular weight of 97 kDa. USP13 is a member of the peptidase C19 family and the human protein shares 96% amino acid sequence identity with its mouse ortholog. Recombinant USP13 has weak deubiquitinase activity against unanchored polyubiquitin chains, but will disassemble tetraubiquitin chains in extended reactions, particularly K63 and K48 linkages. Suggested biological targets of USP13 include PTEN, BECN1, MITF, SKP2 and USP10, and roles for USP13 in melanoma and colorectal cancer have been reported. This recombinant protein contains a C-terminal 6-His tag.
- Xiang, S. et al. (2015) Oncol Rep. 33: 1342
- Zhang, J. et al. (2013) Nat Cell Biol. 15: 1486
- Zhang, Y-H. et al. (2011) PLoS One 6: e29362
- Zhao, X. et al. (2011) Nat Commun. 2: 414. doi: 10.1038/ncomms1421
Citation for Recombinant Human His6-USP13 Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products.The data collected includes not only links to publications in PubMed,but also provides information about sample types, species, and experimental conditions.
1Citation: Showing 1 - 1
- Deubiquitinase USP13 dictates MCL1 stability and sensitivity to BH3 mimetic inhibitorsAuthors: S Zhang, M Zhang, Y Jing, X Yin, P Ma, Z Zhang, X Wang, W Di, G ZhuangNat Commun, 2018;9(1):215.Species: HumanSample Types: Recombinant ProteinApplications: Bioassay
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对重复性要求不高的试验，一般没有问题的，只不过可能需要增加一些转染试剂用量了。
为了避免这个问题，推荐收到货之后，进行适当分装，用多少，拿出来多少最好。
—DNA转染试剂Polyjet-适用于普遍的哺乳动物细胞
LipoD293-适用于悬浮细胞的转染（包括昆虫细胞SF9）-对于普遍的哺乳动物细胞具有更优异的转染效率
—DNAandsiRNA转染试剂
Lipojet-适用于大多数哺乳动物细胞的转染-低毒性（无需换液）-用量少-DNA/RNA共转染（co-transfection）-效果优于Lipofectamine2000
—siRNA转染试剂
GenMute-适用于大多数哺乳动物细胞转染-低毒性（无需换液）-用量少-DNA/siRNA共转染（co-transfection）PepMute-适用于普遍的哺乳动物细胞的转染
想用RNAiMAX或者lipo2000转染siRNA，但是看了下转染试剂的说明书和锐博的siRNA说明书，觉得分别对siRNA的用量描述差别挺大的呀，不知道到底该参考哪个呢。
以24孔板为例在siRNA说明书中写到，siRNA终浓度是50nM的话，加入浓度为20μM的siRNA1.25ul，每孔体积是500ul，那这样的话，每孔最终siRNA的量是25pmol。
但是在RNAiMAX或者是lipo2000说明书中，一个写的每孔siRNA用量是5pmol，一个是500ng，这与siRNA厂家所提供的量相差也太多了吧。
到底该看哪一个呢。
ps.一旦siRNA的量和体积确定下来之后，转染试剂的量和siRNA1:1的加就可以了吗？
请各位大神解答。
1.siRNA说明书中的用量，红线圈出
2.RNAIMAX说明书中siRNA的用量。
3.lipo2000说明书中siRNA用量
但是在选择的时候，也需要注意其他的一些问题。
1、采用何种原料和抗体，是否高效、灵敏、特异
2、规范包被操作，吸附是否均匀
3、重复性、可靠性
6、是否提供技术服务
7、适用于血浆、血清、组织匀浆液、细胞培养上清液、尿液等多种类型的样本
8、可检测动物类型是否丰富
9、可检测指标是否齐全
elisa试剂盒就查下博欧特生物
使用方法：
1、 血清：操作过程中避免任何细胞刺激。使用不含热原和内毒素的试管。收集血液后，1000×g离心10分钟将血红细胞迅速小心地分离。
2、 血浆：EDTA、柠檬酸盐、肝素血浆可用于检测。1000×g离心30分钟去除颗粒。
3、 细胞上清液：1000×g离心10分钟去除颗粒和聚合物。
4、 组织匀浆：将组织加入适量生理盐水捣碎。1000×g离心10分钟，取上清液。
5、 保存：如果样品不立即使用，应将其分成小部分-70℃保存，避免反复冷冻。尽可能的不要使用溶血或高血脂血。如果血清中大量颗粒，检测前先离心或过滤。不要在37℃或更高的温度加热解冻。应在室温下解冻并确保样品均匀地充分解冻。
当然，如果考虑成本这块的话，义翘sinofection可以考虑哦~
