
Background
Cell-permeablederivativeofE64-C,aninhibitorofcalpainandothercysteineproteases,suchaspapain,cathepsinBandcathepsinL1,2.Additiontocellculturesshouldbedoneinserum-freemediaasesterasesinserumwillcleavetheethylesterandreducecellpermeABIlity.Typicalworkingconcentrationis0.5-10μg/ml.Inhibitsdegradationofautophagiccargoinsideautolysosomes3.
Application
Inhibitsproteolysisofautophagiccargo
ProductInformation
Purity: >98.0%byHPLC
MW: 342.4
Formula: C17H30N2O5
CASNo. 88321-09-9
PhysicalState: Lyophilizedwhitetooff-whitepowder
Amount: 1mg,5mg
Solubility: 20mg/mLinDMSO,10mg/mLinethanol
Storage: Store,assupplied,at-20°Cforupto2years.Storesolutionsat-20°Cforupto1month.
References
1.EBMCGowanetal.Biochem.Biophys.Res.Commun.1989158:432
2.DWilcoxandRWMasonBiochem.J.1992285:495
3.NMizushimaetal.Cell2010140:313
Documents
ebiomall.com






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明白了么,少年?
反应体系如下:
plasmid10ul
10xKbuffer5ul
KpnI1ul
BamHI1ul注意千万不可多加总酶量必须<4%
ddH20upto50ul
总体积改变加酶量按比例改变.
如果能分解,那小肠液中的消化酶如何大量共存,如果不能
那它如何识别其他蛋白质物质是不是消化酶?

