
Background
BindstotheN-terminaldomainofvalosin-containingprotein(VCP),anessentialproteinforautophagosomematuration.XanthohumolinhibitsthefunctionofVCPtherebyimpairingautophagosomematurationresultinginaccumulationofthemicrotubule-associatedprotein1lightchain3-II(LC3-II)1.Impairsprostatecancercellgrowthandproliferation2.Suppressesgrowthofpancreaticcancercellsviadown-regulationofNotch1signaling3.Cellpermeable.Xanthohumol,aprenylatedchalconefromhop,inhibitsCOX-1andCOX-2activityandshowschemopreventiveeffects.
Application
Impairsautophagosomematuration
ProductInformation
Purity: >98%byTLC
MW: 354.4
Formula: C21H22O5
CASNo. 6754-58-1
PhysicalState: Lyophilizedyellowpowder
Amount: 5mg,25mg
Solubility: 35mg/mLinDMSOorethanol
Storage: Storedesiccatedassuppliedat-20°Cforupto3years.Storesolutionsat-20°Cforupto1month.
References
1.YSasazawaetal.ACSChem.Biol.20127:892
2.RVeneetal.Mol.Med.201218:1292
3.SKunnimalaiyaanetal.Mol.CancerTher.201514:1395
Documents
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明白了么,少年?
反应体系如下:
plasmid10ul
10xKbuffer5ul
KpnI1ul
BamHI1ul注意千万不可多加总酶量必须<4%
ddH20upto50ul
总体积改变加酶量按比例改变.
如果能分解,那小肠液中的消化酶如何大量共存,如果不能
那它如何识别其他蛋白质物质是不是消化酶?

