
Background
Tanespimycin(17-AAG)isasemi-syntheticanalogofgeldanamycinwhichislesstoxicandmorestable1.SelectivelybindstoandinhibitsHSP90fromtumorcellsdisplayinga100-foldhigheraffinityfortumorcell-derivedovernormalcellHSP902.Displaysanti-angiogenicactivity3.Sensitizestumorcellstoapoptosisinresponsetosuchagentsastaxol4andcisplatin5.Cellpermeable.
Application
AutophagyInducer
ProductInformation
Purity: >98%byTLC
MW: 585.7
Formula: C31H43N3O8
PhysicalState: Lyophilizedredtodarkredpowder
Amount: 1mgor5mg
Solubility: >50mg/mlinDMSO;5mg/mLinethanol
Storage: Storedesiccatedat-20°C.Storesolutionsat-20°Cfor3months.
1.TWSchulteetal.CancerChemother.Pharmacol.199842:273
2.AKamaletal.Nature2003425:407
3.GKauretal.Clin.CancerRes.200410:4813
4.DBSolitetal.CancerRes.200363:2139
5.IAVasilevskayaetal.Mol.Pharmacol.200465:235
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明白了么,少年?
反应体系如下:
plasmid10ul
10xKbuffer5ul
KpnI1ul
BamHI1ul注意千万不可多加总酶量必须<4%
ddH20upto50ul
总体积改变加酶量按比例改变.
如果能分解,那小肠液中的消化酶如何大量共存,如果不能
那它如何识别其他蛋白质物质是不是消化酶?

