
Poly-ubiquitylation of target proteins through linkage at K48, is now the most thoroughly studied of the various chain linkages, and we once considered the hallmark of this post-translational modification. It is now clear that many, if not all, poly-Ub chain topologies likely play distinct and important roles in regulating cellular processes. Nevertheless, K48 linkage remains a critical pathway for the cells to maintain homeostasis through proteolytic degradation, and as such remains very intriguing for the study of DUBs that play a role in the degradation, as well as the proteasome itself.
These diubiquitin chains generated from the enzymatic linkage of wild-type ubiquitin through lysine 48. The most distal ubiquitin contains an arginine substitution for the lysine at position 48, limiting chain length.
Info
Source | Human recombinant |
Form | Liquid |
Molecular Weight | 17,139.7 Da |
Affinity Tag | None |
Concentration | Lot specific |
Buffer | 20 mM Tris, pH 7.5, 0.15 M NaCl, 1 mM EDTA |
Storage | -80°C, avoid cycles of freeze/thaw |
Stability | 1 year under recommended storage conditions |
Applications
- Investigation of DUB linkage specificity.
Documents
ebiomall.com






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明白了么,少年?
反应体系如下:
plasmid10ul
10xKbuffer5ul
KpnI1ul
BamHI1ul注意千万不可多加总酶量必须<4%
ddH20upto50ul
总体积改变加酶量按比例改变.
如果能分解,那小肠液中的消化酶如何大量共存,如果不能
那它如何识别其他蛋白质物质是不是消化酶?

