
Overview:
UBE2H or ubiquitin-conjugating enzyme E2H is a member of the E2 ubiquitin-conjugating enzyme family which is located on chromosome 7 (1). UBE2H is known to act on histones and cytoskeletal proteins, both involved in the degenerative pathway of the motor neuron. UBE2H expression is increased during erythroid differentiation of hCD34(+) cells. Tal1 transcription factor, which is essential for the development of the hematopoietic system and plays a role during definitive erythropoiesis in the adult, activates UBE2H expression, whereas Tal1 knock-down reduced UBE2H expression and ubiquitin transfer activity (2).
Gene Aliases:
E2-20K; UBC8; UBCH; UBCH2
Genbank Number:
NM_003344
References:
1. Kaiser, P. et.al: Human ubiquitin-conjugating enzyme homologous to yeast UBC8. J. Biol. Chem. 269: 8797-8802, 1994. 2. Lausen J,Targets of the Tal1 transcription factor in erythrocytes: E2 ubiquitin conjugase regulation by Tal1. J Biol Chem. 2010 Feb 19;285(8):5338-46.
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植物材料 用最精确的方法,称取不超过100mg的植物材料,置于处理过的研钵,加入液氮进行研磨 将研磨得到的粉末,快速转移至无RNase,并经过液氮冷却的2mL离心管(自备),
但是,这样做的前提的,RNA提得要好,浓度要高,这样免得RNA降解到不能用了。因为在有二价阳离子的(DNAseI buffer含有)情况下,RNA在60度以上时必然发生非酶促降解。
想搞清一个问题,如何区别是因为small RNA 引起的调控呢?还是tRNA引起的调控,有没有有效的分离这两种RNA的办法呢?
样品处理:
a. 植物组织:取新鲜或-70℃冻存100mg组织在液氮中研磨,把粉末加入到1ml裂解液中混匀。
b. 动物组织:取新鲜或-70℃冻存100mg组织加1ml裂解液,用组织研磨杵或匀浆器匀浆处理。
c. 贴壁细胞:直接在培养板中加入裂解液裂解细胞,每106细胞加1ml 裂解液。用取样器吹打混匀。

