
Description:
KAPAAdapterDilutionBuffer(25mL)KAPAStrandedRNA-SeqwithRiboErase
Evolvedtofocus.
TheKAPAStrandedRNA-SeqKitwithRiboEraseoffersahigh-quality,comprehensivesolutionfortranscriptomesequencing.Byutilizingatargetedenzymaticmethodfordepletion,ourworkflowenablessuperiorreductionofribosomalRNA(rRNA)andamorecompleterepresentationofthetranscriptome,includingprecursormRNAsandnon-codingRNA(ncRNA).KitsalsocontainKAPAHiFiforhigh-efficiencyandlow-biaslibraryamplification,andincludeastreamlined,“with-bead”protocol.
- Upto99.98%rRNAdepletion*
- FlexIBLeinputof100ng–1μgtotalRNAfromhuman,mouse,orratspecies*
- Robustandreproducibleacrossvariousinputamounts
- Automation-friendlyworkflow
NEW!KAPADual-IndexedAdapterKitsarenowavailable.FormoreinformationonKAPAAdapterKits,scrolldowntotheOrderingsection,ordownloadtheKAPAAdapterandBeadCalculator.
DownloadourAdapterandBeadCalculator
*Dataonfile.
ForResearchUseOnly.Notforuseindiagnosticprocedures.
ProductHighlights
IndustryleADIngrRNAdepletion
- SuperiorrRNAdepletionfromhigh-qualityandFFPEsamples*
- MoreeconomicalNGSsequencingduetodecreasedrRNAreads,providingdeepersequencingoftranscriptsofinterest
Unsurpassedsequencingdataquality
- Detectionofmoregenesanduniquetranscripts
- Accurateandclearidentificationofsplicesitesandalternativegenesplicing
- ImprovedcoverageenablingbetterdetectionofdifficultandGC-richtranscripts
- 0.973)whicharebetterthanlibrariespreparedfromtheequivalentkitsfromIlluminaorNEB.Dataonfile.">
- 0.973)whicharebetterthanlibrariespreparedfromtheequivalentkitsfromIlluminaorNEB.Dataonfile.">
- 0.973)whicharebetterthanlibrariespreparedfromtheequivalentkitsfromIlluminaorNEB.Dataonfile.">
Highlyreproduciblesequencingresults
- Highcorrelationevenbetweendifferenttestingconditions
- Lowsample-to-samplevariationformorereliableresults
Improvedcoverageuniformity
- Uniformdistributionofreadsovereachtranscript
- Minimal5’–3’biasacrosstranscripts
*Dataonfile.
RelatedProducts
Areyousequencinglow-input,FFPEorhighqualityDNA? RNA? CheckouttheseKapaNGSproductstoimproveyourworkflowandresults:

KAPAStrandedmRNA-SeqKits

KAPARNAHyperPrepKitswithRiboErase

KAPAmRNAHyperPrepKits

KAPALibraryAmplificationKits

KAPAHyperPrepKits

KAPALibraryQuantificationKits
Applications:
Applications- WholeTranscriptome
- GeneExpression
- TranscriptDiscoveryandAnnotation
- TotalRNA-Seq
KitSpecificationsandContents/Storage:
KitSpecificationsandContents/StorageKitscanbestoredforupto10monthsat-20ºC.
KitscontainKAPAHybridizationBuffer,KAPAHybridizationOligos(Human/Mouse/Rat),KAPADepletionBuffer,KAPARNaseH,KAPADNaseBuffer,KAPADnase,KAPAFragment,PrimeandEluteBuffer(2X),KAPA1stStrandSynthesisBuffer,KAPAScript,KAPA2ndStrandMarkingBuffer,KAPA2ndStrandSynthesisEnzymeMix,KAPAA-TailingBuffer(10X),KAPAA-TailingEnzyme,KAPALigationBuffer(5X),KAPADNALigase,KAPAPEG/NaClSPRISolution,KAPALibraryAmplificationPrimerMix(10X)andKAPAHiFiHotStartReadyMix(2X).
Components
Specifications
- SpecDescription
- CompatiblePlatformIlluminaHiSeq®,NextSeq®andMiSeq®
- LibraryTypeRNA
- StartingMaterialHigh-qualityTotalRNA
- InputAmount100ng-1µg
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植物材料 用最精确的方法,称取不超过100mg的植物材料,置于处理过的研钵,加入液氮进行研磨 将研磨得到的粉末,快速转移至无RNase,并经过液氮冷却的2mL离心管(自备),
但是,这样做的前提的,RNA提得要好,浓度要高,这样免得RNA降解到不能用了。因为在有二价阳离子的(DNAseI buffer含有)情况下,RNA在60度以上时必然发生非酶促降解。
想搞清一个问题,如何区别是因为small RNA 引起的调控呢?还是tRNA引起的调控,有没有有效的分离这两种RNA的办法呢?
样品处理:
a. 植物组织:取新鲜或-70℃冻存100mg组织在液氮中研磨,把粉末加入到1ml裂解液中混匀。
b. 动物组织:取新鲜或-70℃冻存100mg组织加1ml裂解液,用组织研磨杵或匀浆器匀浆处理。
c. 贴壁细胞:直接在培养板中加入裂解液裂解细胞,每106细胞加1ml 裂解液。用取样器吹打混匀。

