Description:
KAPADual-IndexedAdapterKit,(15µM)KAPAmRNAHyperPrepKits
Single-DayRNA
TheKAPAmRNAHyperPrepKitsutilizenovelchemistrythatenablesthecombinationofenzymaticstepsandfewerreactionpurifications,resultinginatrulystreamlinedsolutionforthepreparationofhigh-qualitymRNA-seqlibraries.mRNAcapturebeadsareusedpriortolibrarypreparation,whichenrichesformaturemRNAovernon-polyadenylatedspecies,suchasribosomal,precursor,andnoncodingRNAs.
Thestrand-specificworkflowisflexIBLe–supportinglibraryconstructionfromlower-inputamountsanddegradedsamples.KitscontainallreagentsrequiredformRNAcaptureandlibrarypreparation,withtheexceptionofKAPAAdapters(availableseparately).Benefitsinclude:
- single-daylibraryconstruction,inclusiveofmRNAcapture
- reducedhands-onandoveralltimethroughfewerenzymaticandreactioncleanups
- flexibleinputof50ng–1µgtotalRNA*
- maintainover99%strandspecificity*
- KAPAPureBeadsincludedforreactionpurifications
KAPARNAHyperPrepKits
KAPARNAHyperPrepKitsleveragethesameCDNAsynthesisandlibrarypreparationworkflowimprovementsasoutlinedfortheKAPAmRNAHyperPrepKits.KAPARNAHyperPrepKitsincludeallenzymesandbuffersrequiredforcDNAlibrarypreparation,butdonotcontainmRNAcapturereagentsorKAPAAdapters.Kitscanbeusedtopreparelibrariesfrom1ng–100ngoftotal,poly(A)-selected,orrRNA-depletedRNA.

NEW!KAPADual-IndexedAdapterKitsarenowavailable. FormoreinformationonKAPAAdapterKits,scrolldowntotheOrderingsection,ordownloadtheKAPAAdapter andBeadCalculator.
DownloadourAdapterandBeadCalculator
*Dataonfile.
ForResearchUseOnly.Notforuseindiagnosticprocedures.
ProductHighlights
Sequencewhatmatters
- WastefewerreadsduetothecombinationofrRNAcarryoverandPCRduplicates
- Identifymoreuniquetranscriptsandgeneswithequivalentsequencing
Achievesuperiorcoverageuniformity
- Obtainmoreuniformdistributionofreadsacrosstranscripts
- ImprovecoverageofdifficultGC-richtranscripts
RelatedProducts
Areyousequencinglow-input,FFPEorhighqualityDNA? RNA? CheckouttheseKapaNGSproductstoimproveyourworkflowandresults:
KAPARNAHyperPrepKitswithRiboErase
KAPAStrandedmRNA-SeqKits
KAPALibraryAmplificationKits
KAPAHyperPrepKits
KAPALibraryQuantificationKits
Applications:
Applications- Geneexpressionanalysis
- Detectionofgenefusions,isoforms,andotherstructuralvariants
- Noveltranscriptidentification,includingnoncodingtranscripts
- SNVdiscovery
- Wholetranscriptome(KAPARNAHyperPrepKitsonly)
- Targetedtranscriptome(KAPARNAHyperPrepKitsonly)
KitSpecificationsandContents/Storage:
KitSpecificationsandContents/StorageEnzymesandbuffersforrRNAdepletion,cDNAsynthesis,andlibrarypreparationcanbestoredforupto10monthsat-20°C.mRNAcapturereagentsandKAPAPureBeadscanbestoredforupto10monthsat4°C.(USonly)
KitsincludereagentsforRNAfragmentation,cDNAsynthesis,andlibrarypreparation.KitswithreagentsformRNAcapturearealsoavailable.

Specifications
- SpecDescription
- CompatibilityPlatformIlluminaHiSeq,NextSeq,andMiSeq
- LibraryTypeRNA
- StartingMaterialRNAHyperPrepKit:High-qualitytotalRNA,mRNA,orrRNA-depletedRNA;mRNAHyperPrepKit:High-qualitytotalRNA
- StartingMaterialRNAHyperPrepKit:1ng–100ng;mRNAHyperPrepKit:25ng–1µg
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1、测浓度和纯度,是否达标
2、跑电泳看条带是否与测的数据相互印证
如果上述两点满足,就不需要纯化,否则需要进一步纯化。
不过根据经验,一般都不需要,我们实验室用的是GNT系列的试剂盒,提取结果就直接进入下游实验了,效果挺稳定
质粒是真核细胞细胞核外或原核生物拟核区外能够进行自主复制的遗传单位,包括真核生物的细胞器(主要指线粒体和叶绿体)中和细菌细胞拟核区以外的环状脱氧核糖核酸(DNA)分子.现在习惯上用来专指细菌(大肠杆菌)、酵母菌和放线菌等生物中细胞核或拟核中的DNA以外的DNA分子.
小鼠没有叶绿体,你可以直接参照小鼠线粒体的提取方法进行提取.
样品处理:
a. 植物组织:取新鲜或-70℃冻存100mg组织在液氮中研磨,把粉末加入到1ml裂解液中混匀。
b. 动物组织:取新鲜或-70℃冻存100mg组织加1ml裂解液,用组织研磨杵或匀浆器匀浆处理。
c. 贴壁细胞:直接在培养板中加入裂解液裂解细胞,每106细胞加1ml 裂解液。用取样器吹打混匀。
人教版高中生物必修二有这个图
植物材料 用最精确的方法,称取不超过100mg的植物材料,置于处理过的研钵,加入液氮进行研磨 将研磨得到的粉末,快速转移至无RNase,并经过液氮冷却的2mL离心管(自备),
目的掌握RNA提取的基本技术,了解RNA提取过程中的各种注意事项。二、原理RNA提取是分子生物学实验中难度较大的实验技术。RNA提取和DNA提取有类似的地方,因为它们都是核酸,都具有较好的水溶性。提取RNA首先破碎细胞,然后用提取液将RNA溶出,反复抽提去除蛋白质 ……





