Description:
KAPASingle-IndexedAdapterKit,SetA(30µM)KAPARNAHyperPrepKitswithRiboErase(HMR)
Single-DayRNA
TheKAPARNAHyperPrepKitswithRiboErase(HMR)utilizenovelchemistrythatenablesthecombinationofenzymaticstepsandfewerreactionpurifications,resultinginatrulystreamlinedsolutionforthepreparationofhigh-qualityrRNA-depletedRNA-seqlibraries.Byutilizingatargetedenzymaticmethodfordepletion,ourworkflowenablesefficientreductionofribosomalRNA(rRNA)andamorecompleterepresentationofthetranscriptome,includingprecursormRNAsandnon-codingRNA(ncRNA).Thestrand-specificworkflowisflexIBLe–supportinglibraryconstructionfromlower-inputamountsanddegradedsamples.KitscontainallreagentsrequiredforrRNAdepletionandlibrarypreparation,withtheexceptionofKAPAAdapters(availableseparately).Benefitsinclude:
- single-daylibraryconstruction,inclusiveofrRNAdepletion
- reducedhands-onandoveralltimethroughfewerenzymaticandreactioncleanups
- flexibleinputof25ng–1µgtotalRNAfromhuman,mouse,orratspecies*
- highersuccessrateswithlowerinputanddegradedsamples
- maintainover99%strandspecificity*
- KAPAPureBeadsincludedforreactionpurifications
NEW!KAPADual-IndexedAdapterKitsarenowavailable. FormoreinformationonKAPAAdapterKits,scrolldowntotheOrderingsection,ordownloadtheKAPAAdapterandBeadCalculator.DownloadourAdapterandBeadCalculator
*Dataonfile.
ForResearchUseOnly.Notforuseindiagnosticprocedures.

ProductHighlights
Sequencewhatmatters
- WastefewerreadsduetothecombinationofrRNAcarryoverandPCRduplicates
- Identifymoreuniquetranscriptsandgeneswithequivalentsequencing
Achievesuperiorcoverageuniformity
- Obtainmoreuniformdistributionofreadsacrosstranscripts
- ImprovecoverageofdifficultGC-richtranscripts
Generatehigh-qualitylibrariesfromdegradedsamples
- Inputaslittleas25ngwithFFPEsamples,dependingontotalRNAquality
- Achievelowduplicationratesandhighlyefficient,reproduciblerRNAremovalwithdegradedsamples
- Identifymoreuniquetranscriptsandgeneswithequivalentsequencing
Achievereliableresultswithdegradedinputs
- Attainahigh-degreeofexpressioncorrelationbetweenpairedFFPEandfreshfrozensampleswhichprovidesincreasedconfidenceinsequencedataaccuracy
RelatedProducts
Areyousequencinglow-input,FFPEorhighqualityDNA? RNA? CheckouttheseKapaNGSproductstoimproveyourworkflowandresults:
KAPAmRNAHyperPrepKits
KAPAStrandedmRNA-SeqKits
KAPALibraryAmplificationKits
KAPAHyperPrepKits
KAPALibraryQuantificationKits
Applications:
Applications- Wholetranscriptome
- Geneexpressionanalysis
- Detectionofgenefusions,isoforms,andotherstructuralvariants
- Noveltranscriptidentification,includingnoncodingtranscripts
- SNVdiscovery
KitSpecificationsandContents/Storage:
KitSpecificationsandContents/StorageEnzymesandbuffersforrRNAdepletion,CDNAsynthesis,andlibrarypreparationcanbestoredforupto10monthsat-20°C.KAPAPureBeadscanbestoredforupto10monthsat4°C.(USonly)
KitscontainHybridizationBuffer,HybridizationOligos(HMR),DepletionBuffer,RNaseH,DNaseBuffer,DNase,Fragment,PrimeandEluteBuffer(2X),1stStrandSynthesisBuffer,KAPAScript,2ndMarkingBuffer,2ndStrandSynthesis&A-TailingEnzymeMix,LigationBuffer,DNALigase,PEG/NaClSolution,KAPAPureBeads,LibraryAmplificationPrimerMix(10X),andKAPAHiFiHotStartReadyMix(2X).

Specifications
- SpecDescription
- CompatiblePlatform0616
- LibraryTypeRNA
- StartingMaterialHigh-qualityanddegradedtotalRNA
- InputAmount25ng–1µg
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用醌指纹法描述微生物群落的参数[7]有:(1)醌的类型和不同类型的醌的数目;(2)占优势的醌及其摩尔分数含量;(3)总的泛醌和总的甲基萘醌的摩尔分数之比;(4)醌的多样性和均匀性;(5)醌的总量等。对两个不同的群落,由上述分析所得数据可以计算出另一个参数____非相似性指数(D),用于定量比较两个群落结构的差异。
醌指纹法具有简单快速的特点,近几年来广泛用于各种环境微生物样品(如土壤,活性污泥和其它水生环境群落)的分析。
你可以用你的目的引物,用反转录第一链进行扩增,看看能不能得到目的片段.
但是,这样做的前提的,RNA提得要好,浓度要高,这样免得RNA降解到不能用了。因为在有二价阳离子的(DNAseI buffer含有)情况下,RNA在60度以上时必然发生非酶促降解。
mRNA 数量不详,根据转录数量,加工数量各不相同,故无法得知。










