- Description
- Additional Information
- Readable Documents
- Assay Principle
- Reviews
Key Benefits
- Quick 10 minute assay.
- Can monitor multiple time points to follow kinetics.
- Dual mode, can detect H202 or peroxidase activity.
- One-step, no wash assay.
- Adaptable for High Throughput format.
- Non-destructive cell based assay allows monitoring of additional parameters.
- Applications – Fluorescent Plate Reader.
Additional information
| Kit Size | 500 |
|---|
The Fluoro H2O2 detection kit utilizes a non-fluorescent detection reagent, to detect H2O2. H2O2 oxidizes the detection reagent in a 1:1 stoichiometry to produce a fluorescent product resorufin. This oxidation is catalyzed by Peroxidase in a homogeneous no wash assay system.
The detection reagent can be utilized to detect H2O2 release from cells or enzyme coupled reactions (1-7)

Figure 1. Hydrogen Peroxide titrated in 1X reaction Buffer and measured using the Fluoro H2O2 Kit.
| Document Title |
| Fluoro H2O2Protocol |
| Fluoro H2O2 Datasheet |
| msds.fluoroh2o2 |
| Title | File | Link | Author(s) | Journal | Year; Edition:Pages |
| Deficiency of the Bax gene attenuates denervation-induced apoptosis | http://www.springerlink.com/content/p1n841w124828181/ | P. M. Siu and S. E. Alway | Apoptosis Journal | Vol 11/No 6 June 2006 | |
| Manganese chloride stimulates rat microglia to release hydrogen peroxide | http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6TCR-4P2S92J-1&_user=10&_coverDate=09%2F10%2F2007&_rdoc=1&_fmt=high&_orig=gateway&_origin=gateway&_sort=d&_docanchor=&view=c&_searchStrId=1722767089&_rerunOrigin=scholar.google&_acct=C000050221&_version=1&_urlVersion=0&_userid=10&md5=7f28a0171643f2629b142edec039c748&searchtype=a | Ping Zhang, Angela Hatter and Bin Liu | Toxicology Letters | Volume 173, Issue 2, 10 September 2007, Pages 88-100 -doi:10.1016/j.toxlet.2007.06.013 | |
| Superoxide dismutase protects against apoptosis and alveolar enlargement induced by ceramide | http://ajplung.physiology.org/content/295/1/L44.short | Irina Petrache, Terry R. Medler, Amy T. Richter, Krzysztof Kamocki, Ugonma Chukwueke, Lijie Zhen, Yuan Gu, Jeremy Adamowicz, Kelly S. Schweitzer,1 Walter C. Hubbard, Evgeny V. Berdyshev, Giuseppe Lungarella, and Rubin M. Tuder | American Journal of Physiology, Lung Physiol | July 2008, Vol 295, No 1 L44-L53 | |
| Dual Role of Vitamin C Utilization in NO2-Induced Oxidative Stress in Lung Tissues of Mice | http://www.springerlink.com/content/j0870103v5820t78/ | Yingyi Zhang, Chunyan Ma, Yuhuan Xiao, Chang Liu, Qiang Xu, Yan Wang, Xin Xu and Lin Hao | Bulletin of Environmental Contamination and Toxicology | Vol 84, Number 6, 662-666 - DOI: 10.1007/s00128-010-0021-1 | |
| Cigarette Smoke-Generated Reactive Oxygen Species Impair Adenosine-Mediated Wound Closure in Bronchial Epithelial Cells | http://ajrccm.atsjournals.org/cgi/content/citation/179/1_MeetingAbstracts/A2382 | DS Allen-Gipson, JC Jarrell, and H Alvarez-Ramirez | American Journal of Respiratory and Critical care medicine | 2009; 179: A2382 | |
| Benzyl isothiocyanate-mediated generation of reactive oxygen species causes cell cycle arrest and induces apoptosis via activation of MAPK in human pancreatic cancer cells | http://carcin.oxfordjournals.org/cgi/content/abstract/30/10/1744 | Ravi P. Sahu, Ruifen Zhang, Sanjay Batra, Yan Shi and Sanjay K. Srivastava | Carcinogenesis | 2009 30(10):1744-1753 | |
| Vibration Disrupts Vascular Function in a Model of Metabolic Syndrome | http://www.jstage.jst.go.jp/article/indhealth/47/5/47_533/_article | Kristine Krajnak, Stacey Waugh, Claud Johnson, Roger Miller, Meagan Kiedrowski | Industrial Health | Vol 47 (2009), No 5, pp 533-542 | |
| Inhibition of xanthine oxidase reduces oxidative stress and improves skeletal muscle function in response to electrically stimulated isometric contractions in aged mice | http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T38-52JVX0M-1&_user=10&_coverDate=04%2F07%2F2011&_rdoc=1&_fmt=high&_orig=gateway&_origin=gateway&_sort=d&_docanchor=&view=c&_searchStrId=1722912834&_rerunOrigin=scholar.google&_acct=C000050221&_version=1&_urlVersion=0&_userid=10&md5=daa9b274fb07dfe348917b389f76c96b&searchtype=a | Michael J. Ryan, Janna R. Jackson, Yanlei Hao, Stephen S. Leonard and Stephen E. Alway | Free Radical Biology and Medicine | April 2011, doi:10.1016/j.freeradbiomed.2011.04.002 |
| Reference |
| Anal Biochem 253, 162 (1997) |
| J. Immunol Methods 202, 133 (1997) |
| J Neurochem 79, 266 (2001) |
| Am J Physiol Lung Cell Mol Physiol 281, L993 (2001) |
| Mol Hum Reprod 7, 237 (2001) |
| Willingham MC (1999) Cytochemical methods for the detection of apoptosis. J Histochem Cytochem 47:1101–1109 |
| J Invest Dermatol 112, 751 (1999) |
| Part# | Reagent | Temperature |
| Part # 3011 | 5X Reaction Buffer: 20 ml buffer, pH 7.4 | 2-8°C |
| Part # 4007 | Detection reagent: One vial for 500 assays. | 2-8°C |
| Part # 3012 | Hydrogen Peroxide: 200µL of a stabilized 3% solution. | 2-8°C |
| Part # 6004 | Horseradish Peroxidase: 18.9 Units of enzyme | 2-8°C |
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利用NheI和HindIII双酶切插入目的片段,为什么双酶切有目的片段和切开的质粒,测序却没有?
并且测序后在NheI位点后的序列变成了-GGCTAGGTAC-Kpn位点(CGTTTAAACTTAAGCTTG消失。之前和之后的序列都相符),之间的怎么都没了?
请教高人,这样的问题怎么解决?
多谢
你所说的“测序酶”在一般情况下就是“聚合酶”。
因为目前的的测序方法主要就是借助聚合反应。
当然,有些测序方法(比如曾经的SOLiD系统)是利用连接反应,那么它用的“测序酶”肯定是连接酶。
估计,也就是这个原因有人把它。
两个基因大小分别230kb和450kb,酶切后,450kb的有目的条带,但很弱,230kb的质粒条带亮,其下方有一很微弱条带,用的酶分别是:Ncol和Spel体系是(Takara,两个酶切体系不同,用的官网推荐体系):
NcoI1μl
Spel1μl
10×KBuffer2μl
0.1%BSA2μl
DNA3ul
灭菌水upto20μl37℃4h电泳1h
期待着高手们的指点,谢谢。
【之前构建的4个重组质粒里也有两个出现这样的问题(aagtcc变成agtcc),但是酶切能切出插入的片段】
目前问测序公司得到的答复是样品可能不是单克隆,测序结果是不准确的,建议重新挑取单克隆测序。
不知道还有没有别的方法?
●应用酶水解多肽不会破坏氨基酸,也不会发生消旋化。水解的产物为较小的肽段。向左转|向右转

