- Clear plastic hinged case for use with either 8 or 20 mL vials
- Convenient for storing and transporting vials
- Partitions protect vials
- Includes alpha numerical indexing card
- Extra partitions and index cards can be purchased separately
- Similar to existing M-T Vial Files® that hold either 2 or 4 mL vials
The M-T Vial File® provides a compact, easily accessible means of sample storage and has been a popular item for Wheaton. As a result, two new M-T Vial Files® have been created which allows users to store either 8 or 20 mL vials in a similar fashion. The clear polystyrene case has a hinged lid that allows the vials to be covered and protected. The partitions are designed to provide extra protection for the vials. The alpha numerical indexing card allows for identifi cation of each sample vial. The M-T Vial File® comes with polystyrene case, partitions, and indexing card. Partitions and index cards can also be purchased separately.
| SKU | W228790 |
|---|---|
| Bottle / Vial Style | Sample |
| Accessories | Yes |
Product Literature
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利用NheI和HindIII双酶切插入目的片段,为什么双酶切有目的片段和切开的质粒,测序却没有?
并且测序后在NheI位点后的序列变成了-GGCTAGGTAC-Kpn位点(CGTTTAAACTTAAGCTTG消失。之前和之后的序列都相符),之间的怎么都没了?
请教高人,这样的问题怎么解决?
多谢
你所说的“测序酶”在一般情况下就是“聚合酶”。
因为目前的的测序方法主要就是借助聚合反应。
当然,有些测序方法(比如曾经的SOLiD系统)是利用连接反应,那么它用的“测序酶”肯定是连接酶。
估计,也就是这个原因有人把它。
两个基因大小分别230kb和450kb,酶切后,450kb的有目的条带,但很弱,230kb的质粒条带亮,其下方有一很微弱条带,用的酶分别是:Ncol和Spel体系是(Takara,两个酶切体系不同,用的官网推荐体系):
NcoI1μl
Spel1μl
10×KBuffer2μl
0.1%BSA2μl
DNA3ul
灭菌水upto20μl37℃4h电泳1h
期待着高手们的指点,谢谢。
【之前构建的4个重组质粒里也有两个出现这样的问题(aagtcc变成agtcc),但是酶切能切出插入的片段】
目前问测序公司得到的答复是样品可能不是单克隆,测序结果是不准确的,建议重新挑取单克隆测序。
不知道还有没有别的方法?
●应用酶水解多肽不会破坏氨基酸,也不会发生消旋化。水解的产物为较小的肽段。向左转|向右转

