| Product Name | pro - NPY peptide (34-43), humanQXL®520 -RQRYGKRSSP -K(5 - FAM) - NH2 |
| Size | 0.1 mg |
| Catalog # | AS-65602 |
| US$ | $205 |
| Purity | >95% |
This peptide corresponds to host derived pro-NPY sequence with a cathepsin cleavage site. Host proteases, such as cathepsin L are involved in the processing and biosynthesis of neuropeptides including pro-NPY. In viral propagation, cathepsin mediated cleavage of viral proteins is necessary for cell entry, thereby affecting the host protein derived pro-NPY.The peptide is labeled with QXL®520 and 5-FAM as FRET pair and thus can serve as a substrate for investigation enzyme cleavage.Neuropeptide Y (NPY) functions as a peptide neurotransmitter and as a neuroendocrine hormone. The active NPY peptide is generated in secretory vesicles by proteolytic processing of pro-NPY. | |
| Detailed Information | |
| Storage | -20 °C |
| References | 1. Elshabrawy HA, ET AL. Identification of a broad-spectrum antiviral small molecule against severe acute respiratory syndrome coronavirus and Ebola, Hendra, and Nipah viruses by using a novel high-throughput screening assay. J Virol. 2014;88(8):43534365. 2. Funkelstein L, et al. Cathepsin L participates in the production of neuropeptide Y in secretory vesicles, demonstrated by protease gene knockout and expression. J. Neurochem. 2008; 106:384391. |
| QXL®520- RQRYGKRSSP- K(5-FAM)-NH2 | |
| Sequence(Three-Letter Code) | QXL®520 -Arg - Gln - Arg - Tyr - Gly - Lys - Arg - Ser - Ser - Pro - Lys(5 - FAM) - NH2 |
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利用NheI和HindIII双酶切插入目的片段,为什么双酶切有目的片段和切开的质粒,测序却没有?
并且测序后在NheI位点后的序列变成了-GGCTAGGTAC-Kpn位点(CGTTTAAACTTAAGCTTG消失。之前和之后的序列都相符),之间的怎么都没了?
请教高人,这样的问题怎么解决?
多谢
你所说的“测序酶”在一般情况下就是“聚合酶”。
因为目前的的测序方法主要就是借助聚合反应。
当然,有些测序方法(比如曾经的SOLiD系统)是利用连接反应,那么它用的“测序酶”肯定是连接酶。
估计,也就是这个原因有人把它。
两个基因大小分别230kb和450kb,酶切后,450kb的有目的条带,但很弱,230kb的质粒条带亮,其下方有一很微弱条带,用的酶分别是:Ncol和Spel体系是(Takara,两个酶切体系不同,用的官网推荐体系):
NcoI1μl
Spel1μl
10×KBuffer2μl
0.1%BSA2μl
DNA3ul
灭菌水upto20μl37℃4h电泳1h
期待着高手们的指点,谢谢。
【之前构建的4个重组质粒里也有两个出现这样的问题(aagtcc变成agtcc),但是酶切能切出插入的片段】
目前问测序公司得到的答复是样品可能不是单克隆,测序结果是不准确的,建议重新挑取单克隆测序。
不知道还有没有别的方法?
●应用酶水解多肽不会破坏氨基酸,也不会发生消旋化。水解的产物为较小的肽段。向左转|向右转

