Product manufactured in Canada by StressMarq.
Assay Background
8-hydroxy-2-deoxy Guanosine (8-OH-dG) is produced by the oxidative
8-OHdG ELISA Assay Kit
The 8-OHdG ELISA Assay Kit is For Research Use Only
Sensitivity: 0.59 ng/mL
Dynamic Range: 0.94 – 60 ng/mL
Incubation Time: 1 hour
Sample Type: Cell lysates, Plasma, Sample matrices, Urine
Sample Size: 50 μL
Product manufactured in Canada by StressMarq.
Assay Background
8-hydroxy-2-deoxy Guanosine (8-OH-dG) is produced by the oxidative damage of DNA by reactive oxygen and nitrogen species and serves as an established marker of oxidative stress. Hydroxylation of guanosine occurs in response to both normal metabolic processes and a variety of environmental factors (i.e., anything that increases reactive oxygen and nitrogen species). Increased levels of 8-OH-dG are associated with the aging process as well as with a number of pathological conditions including cancer, diabetes, and hypertension. In complex samples such as plasma, cell lysates, and tissues, 8-OH-dG can exist as either the free nucleoside or incorporated in DNA. Once the blood enters the kidney, free 8-OH-dG is readily filtered into the urine, while larger DNA fragments remain in the bloodstream. Because of the complexity of plasma samples, urine is a more suitable matrix for the measurement of free 8-OH-dG than plasma. Urinary levels of 8-OH-dG range between 2.7-13 ng/mg creatine, while plasma levels of free 8-OH-dG have been reported to be between 4-21 pg/ml as determined by LC-MS.
Storage and Stability
This kit will perform as specified if the components are stored as directed and used before the expiration date indicated on the outside of the box. All reagents are stable as supplied at 4°C, except the standard, which should be stored at -20°C. For optimum storage, the 8-OHdG Standard should be aliquoted into smaller portions and then stored appropriately. Avoid repeated freeze/thaw cycles (10 µL of Standard can prepare a triplicate standard curve.
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看有关产前诊断的一个资料，提到：“凡是酶活性能在成纤维细胞中表达均可用绒毛或羊水细胞的酶活性测定进行产前诊断，如溶酶体贮积症。”
不太理解，为什么成纤维细胞中表达的酶就能在绒毛或羊水细胞中测出来呢？绒毛的细胞跟成纤维细胞没什么直接关系吧？或者是因为成纤维细胞脱落到羊水中，破裂，然后就可以检测了？
求各位解答，谢谢。
如癌症的CA抗原系列、用于测胆汁酸、尿酸之类的酶。
转换酶中的一类。催化氨基酸和a-氧代酸（a-酮酸
)或醛酸之间的氨基转换反应，生成与原来的a-氧代酸或醛酸相应的a-氨基酸或ω-
氨基酸，原来氨基酸转变成相应的氧代酸。转氨酶催化的反应都是可逆的。转氨酶可按底物的不同分成3大类。L-a-氨基酸（酮酸转氨酶） 、ω-
氨基酸（酮酸转氨酶）和D-氨基酸转氨酶。转氨酶的辅基是磷酸吡哆醛或磷酸吡哆胺，两者在转氨基反应中可互相变换。
转氨酶参与氨基酸的分解和合成。氨基酸转氨后生成的酮酸或醛酸可经氧化分解而供能，也可转变成糖类或脂肪酸。相反，酮酸或醛酸也可经转氨酶的作用而生成非必需氨基酸。某些氨基酸之间的互变也有转氨酶参与。在高等动物各组织中，活力最高的转氨酶是谷氨酸
： 草酰乙酸转氨酶（ GOT ）和谷氨酸：丙酮酸转氨酶（GPT）。
转氨酶是人体代谢过程中必不可少的“催化剂”，主要存在于肝细胞内。当肝细胞发生炎症、坏死、中毒等，造成肝细胞受损时，转氨酶便会释放到血液里，使血清转氨酶升高。
通常，体检中主要检查的转氨酶有丙氨酸转氨酶（ALT，俗称谷丙转氨酶）和天门冬氨酸转氨酶（AST，俗称谷草转氨酶），其中尤以前者（ALT）最为常用。1%的肝脏细胞损害，可以使血中ALT的浓度增加1倍。因此，ALT水平可以比较敏感地监测到肝脏是否受到损害。
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