Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | ||
|---|---|---|---|---|---|---|
| Plasmid | 111067 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $75 | Add to Cart | |
| AAV5 | 111067-AAV5 | Virus (100 µL at titer ≥ 7×10¹² vg/mL)and Plasmid.More Information | Add to Cart | |||
This material is available to academics and nonprofits only.
Backbone
- Vector backbonepAAV-CAG(Search Vector Database)
- Backbone sizew/o insert(bp)5020
- Total vector size (bp)7063
- Vector typeAAV
Growth in Bacteria
- Bacterial Resistance(s)Ampicillin
- Growth Temperature30°C
- Growth Strain(s)NEB Stable
- Copy numberHigh Copy
Gene/Insert
- Gene/Insert namedLight1.1
- SpeciesSynthetic
- Insert Size (bp)2043
- GenBank IDMH244549
- Tag/ Fusion Protein
- Flag tag (N terminal on insert)
Cloning Information
- Cloning methodRestriction Enzyme
- 5′ cloning siteBaMHI(not destroyed)
- 3′ cloning siteHindIII(not destroyed)
- 5′ sequencing primerGCT CTA GAG CCT CTG CTA ACC ATG TTC
- 3′ sequencing primercgggaagcaatagcatgatacaaaggc (Common Sequencing Primers)
Resource Information
- Supplemental Documents
- pAAV-CAG-dLight1.1.gb
- Addgene Notes
- Addgene Diagnostic Digest
- Terms and Licenses
- UBMTA
- Industry Terms
- Not Available to Industry
- Article Citing this Plasmid
- 1 Reference
Depositor Comments
Addgene NGS identified chicken beta-actin promoter, but not the CMV enhancer and chimeric intron often associated with CAG promoters.Please see the associated diagnostic digest for plasmid verification.
Information for AAV5 (Catalog # 111067-AAV5)(Back to top)
Purpose
Ready-to-use AAV5 particles produced from pAAV-CAG-dLight1.1 (#111067). In addition to the viral particles, you will also receive purified pAAV-CAG-dLight1.1 plasmid DNA.
CAG-driven expression of dLight1.1 dopamine sensor. This sensor is recommended for photometry in vivo. These AAV preparations are suitable purity for injection into animals.Delivery
- Volume100 µL
- Titer≥ 7×10¹² vg/mL
- Pricing$350 USD for preparation of 100 µL virus + $30 USD for plasmid.
- StorageStore at -80℃. Thaw just before use and keep on ice.
- ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.
Viral Production & Use
- Packaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAV5 cap gene
- BufferPBS + 0.001% Pluronic F-68 + 200 mM NaCl
- SerotypeAAV5
- PurificationIodixanol gradient ultracentrifugation
Biosafety
Requestor is responsible for compliance withtheir institution"s biosafety regulations.Lentivirus is generally considered BSL-2. AAV isgenerally considered BSL-1, but may requireBSL-2 handling depending on the insert.Biosafety Guide
Resource Information
- Terms and Licenses
- Terms of Use for Viral Vectors
- Industry Terms
- Not Available to Industry
Viral Quality Control
- Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.
- Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.
Visit our viral production page for moreinformation.
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看有关产前诊断的一个资料,提到:“凡是酶活性能在成纤维细胞中表达均可用绒毛或羊水细胞的酶活性测定进行产前诊断,如溶酶体贮积症。”
不太理解,为什么成纤维细胞中表达的酶就能在绒毛或羊水细胞中测出来呢?绒毛的细胞跟成纤维细胞没什么直接关系吧?或者是因为成纤维细胞脱落到羊水中,破裂,然后就可以检测了?
求各位解答,谢谢。
如癌症的CA抗原系列、用于测胆汁酸、尿酸之类的酶。
转换酶中的一类。催化氨基酸和a-氧代酸(a-酮酸
)或醛酸之间的氨基转换反应,生成与原来的a-氧代酸或醛酸相应的a-氨基酸或ω-
氨基酸,原来氨基酸转变成相应的氧代酸。转氨酶催化的反应都是可逆的。转氨酶可按底物的不同分成3大类。L-a-氨基酸(酮酸转氨酶) 、ω-
氨基酸(酮酸转氨酶)和D-氨基酸转氨酶。转氨酶的辅基是磷酸吡哆醛或磷酸吡哆胺,两者在转氨基反应中可互相变换。
转氨酶参与氨基酸的分解和合成。氨基酸转氨后生成的酮酸或醛酸可经氧化分解而供能,也可转变成糖类或脂肪酸。相反,酮酸或醛酸也可经转氨酶的作用而生成非必需氨基酸。某些氨基酸之间的互变也有转氨酶参与。在高等动物各组织中,活力最高的转氨酶是谷氨酸
: 草酰乙酸转氨酶( GOT )和谷氨酸:丙酮酸转氨酶(GPT)。
转氨酶是人体代谢过程中必不可少的“催化剂”,主要存在于肝细胞内。当肝细胞发生炎症、坏死、中毒等,造成肝细胞受损时,转氨酶便会释放到血液里,使血清转氨酶升高。
通常,体检中主要检查的转氨酶有丙氨酸转氨酶(ALT,俗称谷丙转氨酶)和天门冬氨酸转氨酶(AST,俗称谷草转氨酶),其中尤以前者(ALT)最为常用。1%的肝脏细胞损害,可以使血中ALT的浓度增加1倍。因此,ALT水平可以比较敏感地监测到肝脏是否受到损害。
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