M. tuberculosis Topo I
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M.tuberculosis Topo I
Mycobacterium tuberculosis topoisomerase I is a bacterial type I topoisomerase which can relax supercoiled DNA. It is a potential target for antibacterial drugs and can be used for screening potential novel antibacterial compounds. It is prepared by overexpressing the protein in E. coli and then purified by chromatography.
It is supplied in Dilution Buffer. Store at -80 °C.
The enzyme is supplied with Assay Buffer (5X concentration) and Dilution Buffer (1X) which are also available separately. 1 U of topo I will relax 0.5 µg of supercoiled pBR322 DNA in 30 minutes at 37 °C.
See technical documents below for more detailed information and lot specific activities.
Technical Documents
M.tuberculosis Topo I Relaxation Kit 1
These contain the enzyme and the supercoiled DNA substrate in addition to the Assay and Dilution buffers for relaxation reactions. 1 U of topo I will relax 0.5 µg supercoiled pBR322 DNA in 30 minutes at 37°C.
Technical Documents
High/Medium-Throughput Assay Kit - M.tuberculosis Topo I
The kit is supplied with sufficient M.tuberculosis topo I enzyme, plasmid DNA substrate, buffers and other assay components* for 100 assays. The enzyme is supplied at a minimum concentration of 5 U/μl in Dilution Buffer. The kit is also supplied with sufficient wash buffers for one 96-well plate. These buffers are supplied as 20X concentrates and must be diluted with ultra pure water prior to use. The streptavidin-coated plate is also included.
More information about this assay can be found on the "Services" page under "High/Medium Throughput Assay".
Kit issued with limited licence for individual use only.
Patent held by Inspiralis Ltd., Norwich, Norfolk, UK. (Patent No. GB0424953.8, US7838230)
Technical Documents
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脂代谢及高脂血症的检查
血浆蛋白质检查
诊断酶学
体液平衡紊乱及其检查
1、酶在医药方面的应用
酶在医药方面的应用多种多样,可归纳为下列3个方面:
(1) 用酶进行疾病的诊断
(2) 用酶进行疾病的治疗
(3) 用酶制造各种药物
1.1酶在疾病诊断方面的应用
(1)根据体内酶活力的变化诊断疾病:
表9-1 通过酶活力变化进行疾病诊断
酶
疾病与酶活力变化
淀粉酶
胰脏疾病,肾脏疾病时升高;肝病时下降
胆碱酯酶
肝病、肝硬化、有机磷中毒、风湿等,活力下降
酸性磷酸酶
前列腺癌、肝炎、红血球病变时,活力升高
碱性磷酸酶
佝偻病、软骨化病、骨瘤、甲状旁腺机能亢进时,活力升高;软骨发育不全等,活力下降
谷丙转氨酶
肝病、心肌梗塞等,活力升高
谷草转氨酶
肝病、心肌梗塞等,活力升高
γ-谷氨酰转肽酶(γ-GT)
原发性和继发性肝癌,活力增高至200单位以上,阻塞性黄疸、肝硬化、胆道癌等,血清中酶活力升高
醛缩酶
急性传染性肝炎、心肌梗塞,血清中酶活力显著升高
精氨酰琥珀酸裂解酶
急、慢性肝炎,血清中酶活力增高
胃蛋白酶
胃癌,活力升高;十二指肠溃疡,活力下降
磷酸葡糖变位酶
肝炎、癌症,活力升高
β-葡萄糖醛缩酶
肾癌及膀胱癌,活力升高
碳酸酐酶
坏血病、贫血等,活力升高
乳酸脱氢酶
肝癌、急性肝炎、心肌梗塞,活力显著升高;肝硬化,活力正常
端粒酶
癌细胞中含有端粒酶,正常体细胞内没有端粒酶活性
山梨醇脱氢酶(SDH)
急性肝炎,活力显著提高
5’-核苷酸酶
阻塞性黄疸、肝癌,活力显著增高
脂肪酶
急性胰腺炎,活力明显增高,胰腺癌、胆管炎患者,活力升高
肌酸磷酸激酶(CK)
心肌梗塞,活力显著升高;肌炎、肌肉创伤,活力升高
α-羟基丁酸脱氢酶
心肌梗塞、心肌炎,活力增高
单胺氧化酶(MAO)
肝脏纤维化、糖尿病、甲状腺机能亢进,活力升高
磷酸己糖异构酶
急性肝炎,活力极度升高;心肌梗塞、急性肾炎,脑溢血,活力明显升高
鸟氨酸氨基甲酰转移酶
急性肝炎,活力急速增高;肝癌,活力明显升高
乳酸脱氢酶同工酶
心肌梗塞、恶性贫血,LDH1增高;白血病、肌肉萎缩,LDH2增高;白血病、淋巴肉瘤、肺癌,LDH3增高;转移性肝癌、结肠癌,LDH4增高;肝炎、原发性肝癌、脂肪肝、心肌梗塞、外伤、骨折,LDH5增高
葡萄糖氧化酶
测定血糖含量,诊断糖尿病
亮氨酸氨肽酶(LAP)
肝癌、阴道癌、阻塞性黄疸,活力明显升高
(2)用酶测定体液中某些物质的变化诊断疾病:
表9-2 用酶测定物质的量的变化进行疾病诊断
酶
测定的物质
用 途
葡萄糖氧化酶
葡萄糖
测定血糖、尿糖,诊断糖尿病
葡萄糖氧化酶+过氧化物酶
葡萄糖
测定血糖、尿糖,诊断糖尿病
尿素酶
尿素
测定血液、尿液中尿素的量,
诊断肝脏、肾脏病变
谷氨酰胺酶
谷氨酰胺
测定脑脊液中谷氨酰胺的量,
诊断肝昏迷、肝硬化
胆固醇氧化酶
胆固醇
测定胆固醇含量,诊断高血脂等
DNA聚合酶
基因
通过基因扩增,基因测序,
诊断基因变异、检测癌基因
1.2酶在疾病治疗方面的应用
表9-3 酶在疾病治疗方面的应用
酶 名
来 源
用 途
淀粉酶
胰脏、麦芽、微生物
治疗消化不良,食欲不振
蛋白酶
胰脏、胃、植物、微生物
治疗消化不良,食欲不振,消炎,消肿,除去坏死组织,促进创伤愈合,降低血压
脂肪酶
胰脏、微生物
治疗消化不良
三种酶分别反映肝脏的不同病理过程:
ALT增高往往说明肝实质细胞的损伤;GGTP增高可以说明胆汁淤积和对肝的“诱导作用”;测定CHE可以检查肝脏的蛋白合成功能。