Vial Rack, Solid Aluminum, for 2 ml Microvials
This solid aluminum vial rack for 2ml vials offers superior temperature control during beadbeating than bead mill cell disrupters relying on flowing air chilled by dry-ice or refrigeration. It is well-known that chilled air has much poorer heat conductivity than chilled metal. The block is pre-cooled in a deep freezer or, to cryo-temperatures with dry-ice or liquid nitrogen. Having dimensions of a standard deep-well microplate, it is compatible with not only our MiniBeadbeater-96 but with several competitor microplate shakers.
Our Price : $0.00
An important accessory for the MiniBeadbeater-96 when temperature control is a concern**. Typically, a vial filled with beads, sample and extraction media warms up ~10 degrees per minute of beadbeating. Our 2 ml vial holder (capacity 48 vials) is machined out of high-heat-conductivity aluminum.
The vial holder is pre-cooled to low temperatures inside a refrigerator, a -80º C freezer, or with liquid nitrogen. During the beadbeating process, the chilled vial holder keeps the sample temperature at desired low temperatures.
A related application is 'dry' bead-grinding of fresh tissue by the cryopulverization technique. The sample, beads, vials, and vial holder are all pre-chilled to liquid nitrogen temperatures before being processed in a Mini-BeadBeater-96.
** Helpful Note: Nucleic acid extraction of microorganisms or tissues in the presence of nucleic acid extraction media generally does not require the above temperature control.
Dimensions: 5 x 3.375 x 1.625 inches
Weight: 1.15 pounds
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R: Reversed primer 反向引物
非要说差别,可能就在测序引物是让片段线性增加,PCR引物是让片段指数增加。
在这里我向大家隆重推荐一个在线的引物设计软件,是斯坦福大学的,我认为是最好的在线引物设计软件,我用它设计接近100多对引物,可以说是屡试不爽。它用起来也很简单,最重要的是效果非常好,考虑的因素也非常的多。
先不说那么多了:网址:http://www.yeastgenome.org/cgi-bin/web-primer
下面我继续图示。
帖子发表之后很多战友PM我,让我帮设计引物,其实这很让我失望的,我的本意是让大家掌握这种方法,而不是告诉大家我是一个专家,其实我水平也有限,所以我不会帮大家设计引物,我认为这是科研工作的一部分、基本功,祝大家好运、实验顺利。
引物是一小段单链DNA或RNA,引物可以做为DNA复制开始时DNA聚合酶的结合位点,在细胞外的条件下,只有通过引物,DNA才可以开始进行复制。
引物是人工合成的两段寡核苷酸序列,一个引物与感兴趣区域一端的一条DNA模板链互补,另一个引物与感兴趣区域另一端的另一条DNA模板链互补。
启动子和引物两者的区别:
启动子是DNA分子上能与RNA聚合酶结合并形成转录起始复合体的区域,在许多情况下,还包括促进这一过程的调节蛋白的结合位点,决定RNA聚合酶转录起始位点的DNA序列。引物是一小段单链DNA或RNA,引物可以做为DNA复制开始时DNA聚合酶的结合位点,在细胞外的条件下,只有通过引物,DNA才可以开始进行复制。引物是人工合成的两段寡核苷酸序列,一个引物与感兴趣区域一端的一条DNA模板链互补,另一个引物与感兴趣区域另一端的另一条DNA模板链互补。
启动子是位于结构基因前的非编码区对转录起调控作用的一段DNA序列。引物则是游离于DNA复制的模板链之外的对DNA复制起调控作用的一小段单链DNA或RNA。