| Product Description | Mouse monoclonal antibody to pp28 of Cytomegalovirus. This antibody originates from ascites fluids and is purified by protein G agarose affinity chromatography. |
| Catalog No. | P1207 |
| Unit Size | 500 µg |
| Species/Class | Mouse IgG2a(k) |
| Clone | CH19 |
| Protein Concentration | 1 mg/ml (A280) |
| Buffer | Phosphate Buffered Saline (PBS) pH 7.4 |
| Purity | Protein G agarose affinity chromatography. |
| Functional Activity | Reactive with pp28 of Cytomegalovirus in immunofluorescence (IFA) and western blot assays at 10 µg/ml. |
| Shipping & Storage | This product is supplied frozen on dry ice. Upon receipt, store at -20°C. Avoid multiple freeze-thaw cycles as product degradation may result. |
| References | Pereira, L., Hoffman, M., Gallo, D. & Cremer, N. (1982). Monoclonal antibodies to human cytomegalovirus. Three cell surface proteins with unique immunologic and electrophoretic properties specify cross-reactive determinants. Infect. Immun. 36, 924-932. Pereira, L., Stagno, S., Hoffman, M. & Volanakis, J. (1983). Cytomegalovirus infected cell polypeptides immune precipitated by children with congenital and perinatal cytomegalovirus infections. Infect. Immun. 39, 100-108. Dondero, D. V. & Pereira, L. (1990). Monoclonal antibody production. In Diagnostic Procedures for Viral, Rickettsial and Chiamydial Infections, pp. 101-124. Edited by R. Emmons & N. Schmidt. Washington, D.C.: American Public Health Association. |
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不过也有用 HRP标记的protein a或g的,前提是你跑的样里没有IgG之类的东西。
2 一抗是属于哪个类或亚类
二抗需与一抗的类别或亚类相匹配。这通常是针对单克隆抗体而言。多克隆抗体主要是IgG类免疫球蛋白,因此相应的二抗就是抗IgG抗体.
单克隆抗体的类别及亚类通常会在产品列表中列出,如果你的一抗是小鼠IgM,那么相应的二抗就应当是抗小鼠IgM,或是抗小鼠IgG抗体。
如果单克隆一抗是小鼠IgG的某一亚类(IgG1,IgG2a,IgG2b,IgG3),那么几乎所有的抗小鼠IgG都可以与之结合,或者你也可以选择专门针对这一亚类的二抗,例如,如果你的一抗是小鼠IgG1,那么你可以选择抗IgG1的二抗,此种抗体在双标记实验中尤其适合。
如果你不知道一抗是哪一类别或亚类,那么抗小鼠IgG是一个不错的选择,因为此种抗体可以识别大多数类型的IgG免疫球蛋白。

