![SMOBIO/[TQ1100] ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX), 200 Rxn/SYBR, no ROX), 200 Rxn</span>
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Description
The ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX) is a ready-to-use reagent with all the essential components for quantitative real-time PCR (qPCR) except primers and template. The master mix features high sensitivity and signal intensity as well as low background and better compatibility with cDNA templates derived directly from reverse transcription reaction mixture. The ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX) contains hot-start Taq polymerase in an optimized buffer with dsDNA specific SYBR green fluorescent dye. This master mix allows for sensitive, precise amplification, real-time tracking of the amplification process, and simultaneous quantification for targeted DNA molecules. With inert smart blue contrast dye, the ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX) is ready-to-use and greatly reduces pipetting errors, while largely improving the reproducibility of the process. The ExcelTaq™ 2X Q-PCR Master Mix is also compatible with a ROX reference dye (Cat. No. CF1000) if recommended by the manufacturer of the qPCR system.
Features
High sensitivity and signal intensity
Smart blue contrast dye as a visual aid for reaction setup
Better compatibility for reverse transcription
Low background
Storage
Protected from light. Aliquot to avoid multiple freeze-thaw cycles.-20°C for 12 months
Sensitivity
The amplification plot of real-time PCR with cDNA template ranged from 25 ng to 1.49 fg in quantity, analyzed by using TQ1100 ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX) for Q-PCR amplification.
Better Compatibility for reverse transcription
ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX) shows better compatibility with reverse-transcription reaction mixture as compared to similar products from A and B brands. Two μl of cDNA directly obtained from reverse-transcription reaction mixture were used in a 20 μl qPCR reaction for the compatibility test.
Specificity
The standard curve (right) and melting curve (left) of TQ1100 ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX).
Contents
Component | Volume | Cat. No |
ExcelTaq™ 2X Q-PCR Master Mix(SYBR, no ROX) | 2 x1 ml | TQ1100 (200 Rxn) |
ExcelTaq™ 2X Q-PCR Master Mix(SYBR, no ROX) | 5 x1 ml | TQ1101 (500 Rxn) |
Storage
Protected from light. Aliquot to avoid multiple freeze-thaw cycles.-20°C for 12 months
Manual
Manual_TQ1100_ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX)
SDS
SDS_TQ1100
Flyer
SMOBIO’s Q-PCR Master Mix- Instrument Compatibility
TaqMan® vs. SYBR® Green Chemistry
How can I choose ExcelTaq™ 2X Q-PCR Master Mix?
What instruments are compatible with ExcelTaq™ 2X Q-PCR Master Mix ?
Product Name | ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX) | ExcelTaq™ 2X Q-PCR Master Mix (SYBR, ROX) | ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX) | ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, ROX) | ExcelTaq™ 2X Q-PCR Master Mix (TaqMan, ROX) |
Cat. No. | TQ1100 | TQ1110 | TQ1200 | TQ1210 | TQ2110 |
Detection chemistry | SYBR | SYBR | SYBR | SYBR | TaqMan |
Blue contrast dye for best convenience | ü | ü | ü | ü | X |
ROX dye for reference | X | ü | X | ü | ü |
Time required for enzyme activation | 10 min | 10 min | 2 min | 2 min | 10 min |
qPCR program | Standard | Standard | Fast and Standard | Fast and Standard | Standard |
Instrument Compatibility* | |||||
Applied Biosystems system: | |||||
5700, 7000, 7300, 7700, and 7900HT | Δ | V | Δ | V | V |
StepOne, StepOnePlus | Δ | V | Δ | V | V |
7500, 7500 Fast, QuantStudio, ViiA7 | Δ | Δ | Δ | Δ | Δ |
BioRad system: | |||||
CFX96 , CFX384 | V | V | V | V | V |
iQ5 | V | V | V | V | V |
Opticon, Opticon 2, Chromo 4 | V | V | V | V | V |
Cepheid system: | |||||
Smart Cycler | V | V | V | V | V |
Eppendorf system: | |||||
Mastercycler ep realplex | V | V | V | V | V |
Roche system: | |||||
LightCycler 480, Nano | V | V | V | V | V |
QIAgen system: | |||||
Rotor-Gene™ 3000, 6000, Q | V | V | V | V | V |
Agilent (Stratagene) system: | |||||
MX3000P, 3005P, 4000P | Δ | Δ | Δ | Δ | Δ |
Illumina system: | |||||
Eco | V | V | V | V | V |
Can you provide the results that using ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX, TQ1100) with different instruments?
Using de novo transcriptome assembly and analysis to study RNAi in Phenacoccus solenopsis Tinsley (Hemiptera: Pseudococcidae)
Satnam Singh,corresponding author Mridula Gupta, Suneet Pandher, Gurmeet Kaur, Neha Goel, and Pankaj Rathore
Sci Rep. 2019; 9: 13710. Published online 2019 Sep 23. doi: 10.1038/s41598-019-49997-y
PMCID: PMC6757040
Reference Gene Selection in Phenacoccus solenopsis Tinsley (Hemiptera: Pseudococcidae) and Their Normalization Impact on Gene Expression in RNAi Studies.
Satnam Singh Suneet Pandher Mridula Gupta Gurmeet Kaur Pankaj Rathore
Journal of Economic Entomology, toy328, Published: 17 October 2018 doi: 10.1093/jee/toy328
Synergistic inhibition effect of TNIK inhibitor KY-05009 and receptor tyrosine kinase inhibitor dovitinib on IL-6-induced proliferation and Wnt signaling pathway in human multiple myeloma cells
Yura Lee, Jung-Il Jung, Kyeong-Yong Park, Soon Ae Kim, Jiyeon Kim Oncotarget. 2017 Jun 20; 8(25): 41091–41101. Published online 2017 Apr 12. doi: 10.18632/oncotarget.17056
PMCID: PMC5522218
Hydroxylation and sulfation of sex steroid hormones in inflammatory liver
Sang R. Lee, Seung-yeon Lee, Sang-yun Kim, Si-yun Ryu, Bae-kuen Park, Eui-Ju Hong J Biomed Res. 2017; 31(5): 437–444. doi: 10.7555/JBR.31.20170031
PMCID: PMC5706436
Loss of endogenous estrogen increases cardiac toxicity by doxorubicin
Young Ho Lee, Sang R. Lee, Sun Woo Kwon, Seung-yeon Lee, Bae-kuen Park, Eui-Ju Hong
Journal of Biomedical Translational Research Vol.18 No.4 pp.146-150 DOI : https://doi.org/10.12729/jbtr.2017.18.4.146
ExcelTaq™ 2X Q-PCR Master Mix
Product Name | ExcelTaq™ 2X Q-PCR Master Mix (SYBR, no ROX) | ExcelTaq™ 2X Q-PCR Master Mix (SYBR, ROX) | ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX) | ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, ROX) | ExcelTaq™ 2X Q-PCR Master Mix (TaqMan, ROX) |
Cat. No. | TQ1100 | TQ1110 | TQ1200 | TQ1210 | TQ2110 |
Detection chemistry | SYBR | SYBR | SYBR | SYBR | TaqMan |
Blue contrast dye for best convenience | ü | ü | ü | ü | X |
ROX dye for reference | X | ü | X | ü | ü |
Time required for enzyme activation | 10min | 10min | 2min | 2min | 10min |
qPCR program | Standard | Standard | Fastand Standard | Fastand Standard | Standard |
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目前大量制备单抗的方法主要有两大系统，一是动物体内生产法，这是国内外实验室所广泛采用；另一是体外培养法。
（1）动物体内生产单抗的方法
迄今为止，通常情况下均采用动物体内生产单抗的方法，鉴于绝大多数动物用杂交瘤均由BALB/c小鼠的骨髓瘤细胞与同品系的脾细胞融合而得，因此使用的动物当然首选BALB/c小鼠。本方法即将杂交瘤细胞接种于小鼠腹腔内，在小鼠腹腔内生长杂交瘤，并产生腹水，因而可得到大量的腹水单抗且抗体浓度很高。可见该法操作简便、经济，不过，腹水中常混有小鼠的各种杂蛋白（包括Ig），因此在很多情况下要提纯后才能使用，而且还有污染动物病毒的危险，故而最好用SPF级小鼠。
（2）体外培养生产单抗的方法
总体上讲，杂交瘤细胞系并不是严格的贴壁依赖细胞（anchoragedependentcell，ADC），因此既可以进行单层细胞培养，又可以进行悬浮培养。杂交瘤细胞的单层细胞培养法是各个实验室最常用的手段，即将杂交瘤细胞加入培养瓶中，以含10-15%小牛血清的培养基培养，细胞浓度以1×106-2×106/ml为佳，然后收集培养上清，其中单抗含量约10-50ug/ml。显然，这种方法制备的单抗量极为有限，无疑是不适用于单抗的大规模生产。要想在体外大量制备单抗，就必须进行杂交瘤细胞的大量（高密度）培养。单位体积内细胞数量越多，细胞存活时间越长，单抗的浓度就越高，产量就越大。
