| Product information | Protocols | References | Technical support | Reviews |
Product overview
| Product name | D-Serine antibody - Rabbit pAb |
| Synonyms | Anti-D-Serine antibodyAnti (R)-Serine antibody |
| Immunogen | Conjugated D-Serine |
| Specificity | When tested in competitive ELISA, the anti-conjugated D-Serine antibody did not show any significant cross reactivity with D-Serine analogs, including L-Serine and D-Threonine. |
| Lot number | 140301 |
Storage
| Form | Liquid |
| Purity | Purified anti-serum |
| Storage buffer | Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze / thaw cycles. |
| Material safety datasheet | Download MSDS |
Protocols
| IF - Cell cultures, Whole mounts, Tissue sections | Dilute antibody with the antibody diluent provided in the STAINperfect immunostaining kit A. Use at 1/250 -1/1000 dilution. Follow the STAINperfect protocol suited to your sample |
| Comments | Optimal working dilutions must be determined by the end-user |
| Restrictions | For research use only |
| Full protocol | Download STAINperfect protocol for D-Serine staining |
| Protocols-at-a-glance |
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| Complete Instructions for Use | Protocol-at-a-glance for cell cultures | Protocol-at-a-glance for whole mounts | Protocol-at-a-glance for tissue sections |
References
Antibody not yet cited. Submit an article and get a 10% discount.
Selected publications on D-Serine:
- Paul P, de Belleroche J. The role of D-serine and glycine as co-agonists of NMDA receptors in motor neuron degeneration and amyotrophic lateral sclerosis (ALS). Front Synaptic Neurosci. 2014 Apr 16;6:10.
- Balu DT et al. D-serine and serine racemase are localized to neurons in the adult mouse and human forebrain. Cell Mol Neurobiol. 2014 Apr;34(3):419-35. doi: 10.1007/s10571-014-0027-z. Epub 2014 Jan 17.
- Klatte K et al. Impaired D-serine-mediated cotransmission mediates cognitive dysfunction in epilepsy. J Neurosci. 2013 Aug 7;33(32):13066-80.
- Otte DM et al. Effects of Chronic D-Serine Elevation on Animal Models of Depression and Anxiety-Related Behavior. PLoS One. 2013 Jun 21;8(6):e67131.
- Martineau M et al. Storage and uptake of D-serine into astrocytic synaptic-like vesicles specify gliotransmission. J Neurosci. 2013 Feb 20;33(8):3413-23. doi: 10.1523/JNEUROSCI.3497-12.2013.
- Van Horn MR, Sild M, Ruthazer ES. D-serine as a gliotransmitter and its roles in brain development and disease. Front Cell Neurosci. 2013 Apr 23;7:39. doi: 10.3389/fncel.2013.00039.
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(1)确定抗体的名字,注意中英文名字、别名、亚型等信息。
(2)确定您的实验类型,Elisa,WB,IHC,ICC,或者是FACS。每种抗体说明书都会列出抗体经验证过适用的实验类型,如果抗体说明书没有提及的应用类型,并不意味着该抗体不适用于此种分析应用类型,而仅是说明尚未经过此种实验验证。
(3)确定实验样本的种属,Human,Mouse,Rat等。我们的抗体说明书都列出该抗体验证过的适用物种,可根据说明书列出已验证的种属来选择适合你实验的抗体。
(4)样本抗原蛋白的结构性质。了解样本抗原蛋白的结构性质有助于选择最合适的抗体,待测样本蛋白的结构域和样本在提取和处理过程中是否会变性,蛋白空间构象的改变,会影响抗体的免疫亲和反应。
(5)单多克隆抗体的选择。一般单克隆抗体特异性强,但亲和力相对小,检测抗原灵敏度相对就低;而多克隆抗体特异性稍弱,但抗体的亲和力强,灵敏度高,但易出现非特异性染色(可以通过封闭等避免)。
2. 二抗选择
(1)种属来源。主要根据一抗种属来源而决定二抗来源,如一抗是小鼠来源,那二抗就买抗小鼠的即可(羊、兔等均可)。
(2)标记物的选择。有HRP、Biotin、荧光素等标记物。免疫组化,WB二抗主要选择HRP,Biotin标记的二抗,而免疫荧光染色可按实验需要选择不同荧光素标记的二抗,如FITC、Cy3、PE等。
不过也有用 HRP标记的protein a或g的,前提是你跑的样里没有IgG之类的东西。





