Overview:
PTPRM is a member of the protein tyrosine phosphatase family and can participate in a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation (1). PTPRM has been shown to mediate cell-cell aggregation through the interaction with another molecule of PTPRM on an adjacent cell. PTPRM can interact with scaffolding protein RACK1/GNB2L1 and this interaction may be necessary for downstream signaling in response to cell-cell adhesion (2). PTPRM has been shown to be expressed in human pulmonary vascular endothelia where it directly binds to VE-cadherin and regulates both the tyrosine phosphorylation state of VE-cadherin and barrier integrity.
Gene Aliases:
RPTPM, RPTPU, PTPRL1, hR-PTPu, R-PTP-MU
Genbank Number:
BC051651
References:
1.Phillips-Mason, P.J. et al: The receptor protein-tyrosine phosphatase PTPmu interacts with IQGAP1. J. Biol. Chem. 2006; 281(8):4903-10.2.Koop, E.A. et al: Receptor protein tyrosine phosphatase mu expession as a marker for endothelial cell heterogeneity; analysis of RPTPmu gene expression using LacZ knock-in mice. Int. J. Dev. Biol. 2003; 47(5):345-54
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非常急！
蛋白质在水溶液中的溶解度是由蛋白质周围亲水基团与水形成水化膜的程度，以及蛋白质分子带有电荷的情况决定的。当用中性盐加入蛋白质溶液，中性盐对水分子的亲和力大于蛋白质，于是蛋白质分子周围的水化膜层减弱乃至消失。同时，中性盐加入蛋白质溶液后，由于离子强度发生改变，蛋白质表面电荷大量被中和，更加导致蛋白溶解度降低，使蛋白质分子之间聚集而沉淀。一般33%硫酸铵为沉淀Ig-G的最适饱和度
有机溶剂沉淀，极性有机溶剂降低介点常数使蛋白质凝集沉淀。eg.甲醇、乙醇、丙酮。
磺基水杨酸也可以沉淀蛋白质，但要加酸调节ph，使之低于血清蛋白的pi，使蛋白质带正电荷，与水杨酸酸根结合生成不溶盐而沉淀。
血清蛋白质均带负电荷，但各种蛋白质带负电荷的程度有所差异，以白蛋白为最多。而血清白蛋白等电点为4.7－4.9。所以把ph调到4.7以下就可以了。
