Exalpha Biologicals produces and sells products for Life Science Research, including monoclonal and polyclonal antibodies, for use in immunohistochemistry, wes 查看更多>
Preparing a cell block from cell lines / cell suspensions.Cell lines or purified cell suspensions are a valuable tool to provide known positive controls provid 查看更多>
DAPI Staining To visualize DNA, incubate fixed samples with 100 ng/ml 4",6"-diamidino-2-phenylindole hydrochloride (DAPI) in PBS for 30 min. Rinse 3x with PBTw 查看更多>
LacZ-cell Staining Materials: 1) 0.5% glutaraldehyde in PBS 2) 20mM K4Fe(CN)6o3H2O 0.44g into 50 ml PBS 3) 20mM K3Fe(CN)6 0.33g into 50 ml PBS 4) X-gal 2% in D 查看更多>
Making Boiled Donkey (or whatever) Serum (BDS) for Blocking You want a final working concentration of 5% Serum. Order the serum from the same company and organ 查看更多>
Steve HahnLast Modified December 1997This method works well when transforming with a plasmid and is rapid. (for highest efficiency transformation, see DMSO tra 查看更多>
1. 细胞凋亡与白血病Activation of Apoptosis in Vivo by a Hydrocarbon-Stapled BH3 HelixSCIENCE 2004,305:1466-1470 通过对BCL-2蛋白家族BID的BH3结构域进行化学修饰,使其容易穿过细胞膜,在活体内研究其激活血癌细胞发生凋 查看更多>
contributed by Patricia TsaoThis protocols allows the use of fluorescence microscopy to visualize epitope-tagged receptors expressed by stable adherent cell li 查看更多>