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Making Boiled Donkey (or whatever) Serum (BDS) for Blocking You want a final working concentration of 5% Serum. Order the serum from the same company and organ
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contributed by Patricia TsaoThis protocols allows the use of fluorescence microscopy to visualize epitope-tagged receptors expressed by stable adherent cell li
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Preparing a cell block from cell lines / cell suspensions.Cell lines or purified cell suspensions are a valuable tool to provide known positive controls provid
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contributed by Patricia TsaoThis protocol will visualize both internalized receptors that were once on the plasma membrane and receptors in the biosynthetic pa
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点击量 179 更新于 2021-09-18
STAINING NONFILAMENTOUS ACTIN WITH VITAMIN-D BINDING PROTEINMaterials1. Gc-globulin (vitamin-D binding protein; Calbiochem 345802), prepare 2 mg/ml stock in PB
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Vital Staining of Chromosomes with Hoechst 33258...
点击量 179 更新于 2021-09-16
Vital Staining of Chromosomes with Hoechst 332581. Hoechst 33258, 10 mg/ml stock in DMSO. 2. Culture medium. 3. 15 ml sterile conical tube. 1. In a 15 ml conic
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