SciRep2014Dec23;4:7583PMID:25533220
SongJ,ChenX,WangM,XingY,ZhengZ,HuS
StateKeyLaboratoryofCardiovascularDisease,FuwaiHospital,NationalCenterforCardiovascularDiseases,ChineseAcademyofMedicalSciencesandPekingUnionMedicalCollege,167ABeilishiRoad,XiChengDistrict,Beijing.100037,China.
主要主题词：
B-Lymphocytes,Regulatory;CellProliferation;EndothelialCells;Exosomes;Myocardium;TransformingGrowthFactorbeta
Themechanismofimmunetoleranceistobefurtherunderstood.ThepresentstudyaimstoinvestigatetheroleoftheCardiacendothelialcell(CEC)-derivedexosomesintheinductionofregulatoryBcells.Inthisstudy,CECswereisolatedfromthemouseheart.Exosomeswerepurifiedfromtheculturesupernatantoftheprimaryendothelialcells.ThesuppressorfunctionsoftheregulatoryBcellsweredeterminedbyflowcytometry.TheresultsshowedthattheCEC-derivedexosomescarriedintegrinv6.Exposuretolipopolysaccharide(LPS)inducedBcellstoexpressthelatenttransforminggrowthfactor(TGF)-,thelatterwasconvertedtotheactiveform,TGF-,bytheexosome-derivedv6.TheBcellsreleasedTGF-inresponsetore-exposuretotheexosomesintheculture,whichsuppressedtheeffectorTcellproliferation.WeconcludethatCEC-derivedexosomeshavethecapacitytoinduceBcellswithimmunesuppressorfunctions.
