RENYa-Na,FANHua-Hua,NIEXiao-Xuan,GAOLi,YANGJie,LIUYan,GAOFengBloodEngineeringLaboratory,ShanghaiBloodCenter,Shanghai200051,ChinaToconfirmthemechanismofexosomesastumorvaccinesinducingimmunityresponse,dendriticcells(DCs)wereinducedfromhumanperipheralbloodmononuclearcells,whileexosomeswereisolatedfromDCloadedtumorantigen.TheeffectofexosomesonprimingTcellproliferationwasanalysedunderconditionswithorwithontDCs,orDCsatdifferentmaturestages.Thefunctionofexosomesinimmunitywasdetectedthroughblocktestafterblockingsomemolecules(CD11a,CD11b,CD11c,CD54,MFG-E8andCD83).TheeffectofDCsonembeddedexosomeswasobservedbyconfocalmicroscopy,theeffectofblockingsurfacemoleculesonexosomesonDC-embeddingexosomeswasassayedbyflowcytometry.TheresultsindicatedthatbothexosomesderivedfromimDC(imDex)andexosomesderivedfrommDC(mDex)couldnotprimeTcellswithoutDCorwithimDC.TheexosomesderivedfrommDCinducedwithdifferentcytokines(LPS,TNF-α,CpG,CD40L)werenosignificantdifferenceinconcentrationsbutweredifferentineffect.TheimmunityfunctionofexosomesdependedonCD11a,CD11b,CD11c,CD54,MFG-E8andCD83molecules,theeffectofprimingTcellsisreducedwhenthesemoleculeswereblocked.ConfocalmicroscopyandFACSassayshowedthatblockingCD11aandCD54couldinhibiteexosome-targetedDCandDC-embeddedexosomes.ItiscondudedthattheexosomestargetDCsthroughtheirsurfacemolecules,thereforeresultsinimmuneresponseofTcells.【Fund】：上海市科委科研计划基金项目(05XD14029)
【CateGoryIndex】：R392.4
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