Cadherins are transmembrane glycoproteins vital in calcium-dependent cell-cell adhesion during tissue differentiation. Cadherins cluster to form foci of homophilic binding units. A key determinant to the strength of the cadherin-mediated adhesion may be by the juxtamembrane region in cadherins. This region induces clustering and also binds to the protein p120 catenin. The cytoplasmic region is highly conserved in sequence and has been shown experimentally to regulate the cell-cell binding function of the extracellular domain of E-cadherin, possibly through interaction with the cytoskeleton. Many cadherins are regulated by phosphorylation, including N-cadherin and E-cadherin. N-cadherin is phosphorylated by c-Src at Tyr-820, Tyr-853, Tyr-860, Tyr-884, and Tyr-886. Phosphorylation of Tyr-860 can disrupt cadherin binding to β-catenin. Since many of these tyrosine sites are conserved in the cadherin family, phosphorylation of these sites may be critical for cadherin function.
References
Western blot image of human endothelial cells untreated (lanes 1 & 3) or treated with pervanadate (1 mM) for 30 min (lanes 2, 4, 5 & 6). The blots were probed with anti-N-Cadherin (Cytoplasmic) (lanes 1 & 2) and anti-N-cadherin (Tyr-820) (lanes 3-6). The latter antibody was used in the presence of no peptide (lane 4), phospho-N-cadherin (Tyr-820) peptide (lane 5), or phospho-N-cadherin (Tyr-860) peptide (lane 6).
Immunocytochemical labeling of phosphorylated N-Cadherin in pervanadate-treated mouse C2C12. The cells were probed with N-Cadherin (Cytoplasmic) and N-Cadherin(Tyr-860) antibodies, followed by immunofluorescent detection.
The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.
*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blotmobilities of known proteins with similar MW.
Product References:
CP1751 Chang, Y.J. et al. (2014) Mol Cell Biol. 34(6):1003 WB: PC12-SH2B1� cellCP1951 Wang, M. et al. (2014) Cell Mol Neurobiol. 34(1):123 WB: MN9D neuronsCP1751 Sato, F. et al. (2012) Intern J Molec Med 30:495. WB: Human pancreatic cancer BxPC-3 cellsCP1751 Wu, Y. et al. (2012) Int J Oncol. 41(4):1337. WB: human pancreatic cancer cells (PANC-1 PaCa-2)CM1701 Wang, T.C. et al. (2011) J. Cell. Physiol. 226:2063. WB, ICC: rat PC12 cellsCP1751 Ferreri, D.M. et al. (2008) Cell Comm Adh. 15(4):333. WB: human, bovine VECsThis kit contains:
| CATALOG# | DESCRIPTION | SIZE | APPLICATIONS | SPECIES REACTIVITY | MW (kDa) |
CM1701 | N-Cadherin (Cytoplasmic) Mouse mAb | 50 μl | WB, E, IP, ICC | Hu, Rt, Ms | 130 |
CP1751 | N-Cadherin (a.a. 811-824) Rabbit pAb | 50 μl | WB, E, IP, ICC | Hu, Rt, Ms | 130 |
CP1801 | N-Cadherin (Tyr-820), phospho-specific Rabbit pAb | 50 μl | WB, E, ICC | Hu, Rt, Ms | 130 |
CP1851 | Unphosphorylated N-Cadherin (Tyr-820) Rabbit pAb | 50 μl | WB, E | Hu, Rt, Ms | 130 |
CP1901 | N-Cadherin (a.a. 853-864)[E-Cadherin (a.a. 828-839)] Rabbit pAb | 50 μl | WB, E | Hu, Rt, Ms | 130 |
CP1951 | N-Cadherin (Y860)[E-Cadherin (Y835)], phospho-specific Rabbit pAb | 50 μl | WB, E, ICC | Hu, Rt, Ms | 130 |
KIT SUMMARY
The N-cadherin phospho-regulation antibody sampler kit can be used to examine phosphorylation of N-cadherin at Tyr-820 and Tyr-860. The kit also includes monoclonal and polyclonal antibodies to monitor total expression levels of N-Cadherin.
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明胶与干酪素都是蛋白质类，其分子结构同属氨基酸，但前者比后者价格便宜很多，而且在我国的产盆很大，因此具有代替干酪素做涂料胶粘剂的可能性。
甲苯二异氰酸酯（TDI）有两种异构体：2，4－甲苯二异氰酸酯和2，6－甲苯二异氰酸酯。甲苯二异氰酸酯是水白色或淡黄色液体，具有强烈的刺激性气味，在人体中具有积聚性和潜伏性，对皮肤、眼睛和呼吸道有强烈刺激作用，吸入高浓度的甲苯二异氰酸酯蒸气会引起支气管炎、支气管肺炎和肺水肿；液体与皮肤接触可引起皮炎。液体与眼睛接触可引起严重刺激作用，如果不加以治疗，可能导致永久性损伤。长期接触甲苯二异氰酸酯可引起慢性支气管炎。对甲苯二异氰酸酯过敏者，可能引起气喘、伴气喘、呼吸困难和咳嗽。
与乙醚、二甘醇、丙酮、四氯化碳、苯、氯苯、煤油、橄榄油混溶。能与含羟基的化合物、水、胺和具有活泼氢原子的化合物反应生成氨基甲酸酯、脲、氨基脲等。甲苯用混酸硝化得到2,4-和2,6-二硝基甲苯，然后在镍催化剂存在下加氢还原得到2,4-和2,6-二氨基甲苯，再在氯苯溶液中与光气反应制得。主要作为聚氨酯树脂的生产原料，用于生产聚氯酯泡抹塑料、涂料、橡胶、粘合剂、密封剂等。也可用作橡胶硫化剂、蛋白质交联剂等。包括泡沫塑料；聚氨酯涂料；聚氨酯橡胶；聚酰亚胺纤维和胶粘剂等也有一些应用。
从交联剂的配制，以及交联反应体系，交联后样品是否沸水煮10min,最后SDS-PAGE检测。
