β-Catenin is a 92 kDa protein that binds to the cytoplasmic tail of E-Cadherin. The cadherins, transmembrane adhesion molecules, are found with catenins at adherens junctions. Deletions in the cytoplasmic domain of E-Cadherin eliminate catenin binding and result in a loss of cell adhesion. Tyrosine phosphorylation of β-Catenin can regulate its interaction with critical components of adherens junctions. Both Fer and Fyn Kinases phosphorylate tyrosine 142 in vitro. Overexpression of these kinases in epithelial cells disrupts interactions between α- and β-Catenins. The phosphorylation of tyrosine 142 may act as a switch from the transcriptional to the adhesive role of β-Catenin. Src family kinases can also phosphorylate tyrosine 86 and 654 in β-Catenin. The Tyr-654 phosphorylation regulates β-Catenin binding to E-cadherin. Thus, site-specific tyrosine phosphorylation of β-Catenin may regulate protein-protein interactions, leading to changes in cell adhesion.
References
Immunocytochemical labeling of phosphorylated β-Catenin in paraformaldehyde-fixed and NP-40-permeabilized rabbit spleen fibroblasts (top row) and A431 cells (bottom row). The fixed cells were labeled with mouse monoclonal antibody to β-Catenin (CM1811) (top and bottom left), and phospho-specific β-Catenin (Tyr-142) (top right) or β-Catenin (Tyr-654) (bottom right). The antibodies were detected using Goat anti-Mouse DyLight® 488 or Goat anti-Rabbit DyLight® 594.
The products are are safely shipped at ambient temperature for both domestic and international shipments. Each product is guaranteed to match the specifications as indicated on the corresponding technical data sheet. Please store at -20C upon arrival for long term storage.
*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blotmobilities of known proteins with similar MW.
Product References:
CP1081 Tsuneki, M. et al. (2014) Mol Cell Biol. 34(24): 4485. (IF: mouse EOMA, brain endothelial cells)CP4021 Lu, Y. et al.(2014) Stem Cells Dev. 23(15):1755. (IF: chicken embryonic fibroblasts)CM1181 Condello, S. et al. (2013) FASEB J 27(8):3100. (WB & IHC: Ovarian Cancer Cells)CM1181 Yakubov, B. et al. (2013) Neoplasia 15(6):609. (WB: Human Ovarian Cancer Cells)CP1081 Qi, F. et al. (2013) Am J Pathol. 183(5):1654. (WB: human mesothelial)CP4021 Condello, S. et al. (2013) FASEB Journal 27(8): 3100. (WB: ovarian cancer cells)CP1081 Krejci, P. et al. (2012) PLoS ONE. 7(4):e35826. (WB: rat chondrosarcoma cells)CP1081 Beard, R.S. et al. (2011) Blood. 118(7):2007. (ELISA: mouse microvascular ECs)This kit contains:
| CATALOG# | DESCRIPTION | SIZE | APPLICATIONS | SPECIES REACTIVITY | ICC DILUTION |
CM1181 | β-Catenin Mouse mAb | 50 μl | WB, E, IP, ICC, IHC | Hu, Rt, Ms | 1:250 |
CP1081 | β-Catenin (Tyr-142)/[γ-Catenin (Tyr-133)], phospho-specific Rabbit pAb | 50 μl | WB, E, ICC | Hu, Rt, Ms | 1:100 |
CP4021 | β-Catenin (Tyr-654)/[γ-Catenin (Tyr-644)], phospho-specific Rabbit pAb | 50 μl | WB, E, ICC | Hu, Rt, Ms, Ck, Fr | 1:200 |
MS3011 | Anti-Mouse Ig:DyLight® 488 Goat pAb | 100 μl | ICC, IHC | Ms | 1:200 |
RS3271 | Anti-Rabbit Ig:DyLight® 594 Goat pAb | 100 μl | ICC, IHC | Rb | 1:200 |
KIT SUMMARY
The β-Catenin phospho-regulation kit can be used for immunocytochemical co-localization of β-Catenin phosphorylation at Tyr-142 and Tyr-654 relative to total β-Catenin. The kit includes rabbit polyclonal and mouse monoclonal antibodies, along with goat-anti-rabbit conjugated to DyLight® 594 and goat anti-mouse conjugated to DyLight® 488 for dual labeling experiments.
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明胶与干酪素都是蛋白质类，其分子结构同属氨基酸，但前者比后者价格便宜很多，而且在我国的产盆很大，因此具有代替干酪素做涂料胶粘剂的可能性。
甲苯二异氰酸酯（TDI）有两种异构体：2，4－甲苯二异氰酸酯和2，6－甲苯二异氰酸酯。甲苯二异氰酸酯是水白色或淡黄色液体，具有强烈的刺激性气味，在人体中具有积聚性和潜伏性，对皮肤、眼睛和呼吸道有强烈刺激作用，吸入高浓度的甲苯二异氰酸酯蒸气会引起支气管炎、支气管肺炎和肺水肿；液体与皮肤接触可引起皮炎。液体与眼睛接触可引起严重刺激作用，如果不加以治疗，可能导致永久性损伤。长期接触甲苯二异氰酸酯可引起慢性支气管炎。对甲苯二异氰酸酯过敏者，可能引起气喘、伴气喘、呼吸困难和咳嗽。
与乙醚、二甘醇、丙酮、四氯化碳、苯、氯苯、煤油、橄榄油混溶。能与含羟基的化合物、水、胺和具有活泼氢原子的化合物反应生成氨基甲酸酯、脲、氨基脲等。甲苯用混酸硝化得到2,4-和2,6-二硝基甲苯，然后在镍催化剂存在下加氢还原得到2,4-和2,6-二氨基甲苯，再在氯苯溶液中与光气反应制得。主要作为聚氨酯树脂的生产原料，用于生产聚氯酯泡抹塑料、涂料、橡胶、粘合剂、密封剂等。也可用作橡胶硫化剂、蛋白质交联剂等。包括泡沫塑料；聚氨酯涂料；聚氨酯橡胶；聚酰亚胺纤维和胶粘剂等也有一些应用。
从交联剂的配制，以及交联反应体系，交联后样品是否沸水煮10min,最后SDS-PAGE检测。
