
Human CD31 is an adhesion molecule expressed on platelets, endothelial cells, leukocytes and their bone marrow precursors. CD31 plays a role in homophilic adhesion and heterophilic transendothelial migration (3).
Isotype: Murine IgG1
Immunogen: Stimulated human leukocytes
Specificity: Antibody 158-2B3 reacts with domain 1 of CD31 (1).
Functional Application: Antibody 158-2B3 blocks homophilic interaction and heterophilic transendothelial migration(1). It has been used for FACS analysis of circulating Endothelial Microparticles (4).
References:
1. Leukocyte Typing VI (T. Kishimoto, et al, eds.) Garland Publishing, Inc., New York (1997) p. 362-372.
2. P.J. Newman, (1997) J Clin Invest 99: 3-10.
3. K.L. Yong, et al, (1998) Blood 91: 1196-1205.
4. AF Tramontano, L Ragolia, et al. (2010) Mediators of Inflammation 2010: 250476 doi:10.1155/2010/250476.
5. I Soaita, W Yin, D A Rubenstein (Jan 2017) Platelets http://dx.doi.org/10.1080/09537104.2016.1260703
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上面较高的是样本曲线,下面较低的是未加样本(茎环引物和引物、探针都加入)
引物,又名引子。是一小段单链DNA或RNA,作为DNA复制的起始点,在核酸合成反应时,作为每个多核苷酸链进行延伸的出发点而起作用的多核苷酸链,之所以需要引物是因为在DNA合成中DNA聚合酶仅仅可以把新的核苷酸加到已有的DNA链上。
引物设计略有不一样,但都可用Primer5设计。
http://medgen.ugent.be/rtprimerdb/

