Mousemonoclonalantibodiescanbeusedforimmunohistochemicaldetectiononmousetissues;however,thebiotinylatedsecondaryantibodyusedfordetectionoftheprimaryantibodywillalsobindtotheendogenousmouseIgGinthetissue,resultinginbackgroundstainingfrombound-mouseIgG.Tocircumventtheproblem,BiocarehasdevelopedtheMMBiotinylationKit.Thekitprovidesallthereagentsnecessarytobiotinylateaprimaryantibody.
Thebasicconceptofthekitisverysimple.Theantibodyismixedwithabiotinylationreagentfor30minutespriortotheapplicationofprimaryantibody.Aslittleas5to10µLofantibodycanbeused.Oncethecomplexisformed,amoppingreagentisaddedandthenthebiotinylatedantibodycomplexisappliedtothetissuespecimen.Thetissueisthenincubatedwiththestrepavidin-peroxidasereagent,andthecolorreactionisdevelopedbyaDABchromogensubstrate.
ThesystemhasbeenspeciallyformulatedtoeliminateendogenousmouseIgGbackgroundstaining.Eventhemostdifficulttissuessuchaslymph,spleen,lungandkidneyarevirtuallybackgroundfree.
EachKitIncludes:
1.DaVinciGreen(PD900H)
2.BackgroundSniper(BS966H)
3.Peroxidazed1(PX968H)
4.BiotinylationReagent(MMBR610F)
5.MoppingReagent(MMMR611F)
6.Streptavidin-HRPLabel(HP604H)
7.DABChromogen(DB851D)
8.DABSubstrateBuffer(DS854H)
9.CATHematoxylin(CATHEH)
10.DABSparkle(DS830H)
11.MixingVial(VL103)
| IntendedUse | RUO |
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ebiomall.com
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上面较高的是样本曲线，下面较低的是未加样本（茎环引物和引物、探针都加入)
引物，又名引子。是一小段单链DNA或RNA，作为DNA复制的起始点，在核酸合成反应时，作为每个多核苷酸链进行延伸的出发点而起作用的多核苷酸链，之所以需要引物是因为在DNA合成中DNA聚合酶仅仅可以把新的核苷酸加到已有的DNA链上。
引物设计略有不一样，但都可用Primer5设计。
http://medgen.ugent.be/rtprimerdb/
