Description:
REAADSAnti-ProthrombinIgG isan anti-ProthrombinantibodyELISAtestkitforthe semi-quantitative,indirectdetectionofantibodiesagainsthumanprothrombin.AsinglepointcalibratorisusedtodetermineIgGanti-prothrombinantibodyconcentrationsinhumanserumofcitratedplasma.
Advantages:
- ConvenientELISAprocedure
- ForthespecificdeterminationofIgGisotype
- Objective,accurateandreproducIBLe
- Reagentcompletekits
- Totalincubationtime:40minutesatroomtemperature
PrincipleandProcedure:
Principle
TheREAADSIgGanti-Prothrombinantibodytestkitsaresemiquantitative,indirectELISAsforthedetectionofantibodiesagainsthumanprothrombin.AsinglepointcalibratorisusedtodetermineIgGanti-prothrombinantibodyconcentrationsinhumanserumofcitratedplasma.
Procedure
Dilutedpatientserumorplasmaisincubatedinmicrowellscoatedwithhumanprothrombin.Anti-Prothrombin(aPT)antibodiesinpatientsampleswillbindtotheimmobilizedantigeninthewells.Afterwashingtoremoveunboundserumorplasmaproteins,antibodiesspecificforhumanIgG,labeledwithhorserADIshperoxidase(HRP),areboundantibodies.Thewellsarewashed,andachromogenicsubstrateisadded,resultinginacolorchangethatisproportionaltotheamountofantibodypresent.Theassayiscompletedbytheadditionofastoppingsolution.ResultsareobtainedbyreadingtheO.D.(opticaldensityorabsorbance)at450nmofeachwellinaspectrophotometer.TheO.D.valuesofcontrolandpatientsamplesaremultipliedbythecalibratorconversionfactortoobtainIgGaPTantibodyconcentrationstimeis40minutesatroomtemperature.
ClinicalPerformance*:
ClinicalSpecificity:serumsamplesfrom100healthyblooddonorsweretestedforaPTantibodies.Againstanestablishedcutoffvalueof20Gand20Munits,thespecificityoftheIgGassaywas95%.Aspresentedinthetablebelow,similarresultswereseenwhenplasmasamplesfrom100healthyblooddonorsweretested.Assayspecificitywith42infectiousdisease(syphilis)patientsand42RheumatoidArthritis(RA)patientsisalsopresentedinthetable.
ClinicalSensitivity:serumsamplesfrom41unselectedSLEpatientsweretestedforIgGaPTantibodies.14.6%ofthesampleswerepositiveforIgGaPTantibodies(meanvaluesof10.4Gunits).Serumsamplesfrom11selectedfemalepatientswithprimaryanti-phospholipidsyndrome(APS)werealsoevaluatedforantibodiestoprothrombin.18.2%ofthesampleswerepositiveforIgGantibodies(meanvaluesof12.3G).
| IgGaPT (%positive) | |
| Healthyserum (n=100) | 5% |
| Healthyplasma (n=100) | 1% |
| Syphilis (n=42) | 4.8% |
| RA (n=42) | 4.8% |
| SLE (n=41) | 14.6% |
| APS (n=11) | 18.2% |
*Dataavailableuponrequest.
Background:
Severalplasmaproteinsassociatedwiththecoagulationsystemandwithstrongphospholipidbindingpropertieshavebeenidentifiedasantiphospholipidcofactors.Antibodiestoprothrombin,oneoftheseplasmaproteins,havebeenreportedinpatientswithantiphospholipidsyndrome.TestingfortheseantibodiesisproposedtobeincludedintheSEROlogicevaluationofantiphospholipidantibodies.Clinically,elevatedserumlevelsofanti-prothrombin(aPT)antibodiesareassociatedwithanincreasedriskforAPS,characterizedbyrecurrentarterialorvenousthrombosis,thrombocytopeniaand/orfetalloss.HighserumorplasmalevelsofaPTantibodiesmayaddvaluableinformationinthelaboratoryassessmentofantiphospholipidantibodies.
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请问下有无同学需要H37RA的？我是做EAE模型的，上个月购买了BDDifco公司的H37RA（货号），因为购买的时候只能整盒6支购买，但我们用不了那么多，所以想问问有无同学需要的，100mg/支，800元/支或用等价试剂交换。地址广州。有需要的请私信，谢谢！
A、Na2CO3HClH2SO4NaNO3
B、K2CO3H2SO4HNO3BaCl2
C、HClHNO3AgNO3NaCl
D、NaOHFeCl3MgCl2BaCl2
我想检测血管组织中的钙离子浓度，不知道哪个公司有试剂盒
2.NaHCO3,Ba(OH)3,H2SO4
3.HCL,NaAlO2,NaHSO4
4.Ca(OH)2,Na2CO3,BaCO3
谢谢了
要原因
bhclna2so4nano3na2co3
chclnaohna2co3nacl
dba(oh)2nahco3alcl3nahso4
C.MgCl2溶液和氨水D.盐酸和NaAlO2溶液
为什么
