UniversalTCellAssayStandardTERS™Kit1
ProductCode:TERS-01
FortheproductionofTCR-engineeredreferencesamplesforperformancemonitoringofT-cellassays.Amount:Sufficientforthetransfectionof40millionPBMCsPurity:RNasefreeMHCRestriction:HLA-A*0201Peptide:NLVPMVATVKitContent:TCRRNAαchain(35µg),TCRRNAβchain(35µg),GFPRNA(50µg),Peptide(50µg)DeliveryFormat:Kitwith4reagentsandinstructionsforuseApplication(s):StandardizationofTcellassays,PerformancemonitoringofTcellassaysIndication(s)/Topic(s):Control,AssayStandardDeliveryTime:2-5daysPleasenote!AnMHCDextramer®(orequivalentmultimer)isrequiredtotesteverybatchofTCR-engineeredreferencesamples.PleaseorderthecorrespondingCMVDextramer™separately!
UniversalTCellAssayStandardTERS™KitGenerateMHC-class-IrestrictedT-cellreceptor(TCR)-engineeredreferencesamplescontainingdefinednumbersoffunctional,antigen-specificTcellsforstandardizationandmonitoringof:
- MHC-Multimerstaining(e.g.Dextramer®)
- intracellularcytokinedetection(ICS);
- ELISPOTassays.
Theyrepresentanidealtoolforvalidatingandmonitoringassayperformancewithinoracrossinstitutions.Thisnewproductmaybeusedtostandardizeantigen-specifictestsovertime,betweendifferentplatforms,ordifferentlaboratories.Itmayalsobeusedtocalibrateantigen-specificassays.SpecificityTERS™Kitscanbeusedtomonitortheperformanceoffunctionalandnon-functionalT-cellassaysirrespectiveofthespecificityselected.Theyareruninthesamewayasusualassaystandards,i.e.inparalleltoothersamplestestedinagivenassayrun.Pleasenote,thatforfunctionaltestinginvolvingT-cellstimulationbyapeptidepresentedonaspecificMHCmolecule,theMHCoftheusedcellmaterialsmustmatchtheMHCrestrictionoftheTERSKitasindicated.IntheeventthatthepeptiderecognizedbytheTERSisthesameasusedforstimulatingothersamplesinanassayrun,anadditionallevelofcontrol(e.g.integrityofthespecificpeptide)isprovided.Ifthisisdesired,youmaywishtocheckifaKitforproducingTERSrecognizingyourspecificpeptideisavailable
ThepreparationofaTERS™batchwiththisproductrequiresperipheralbloodmononuclearcells(PBMCs),whichmaybeobtainedfromahumanbuffycoat,forexample(notsupplied).Additionalreagents,anelectroporatorandaflow-cytometerarerequiredduringtheproductionprocess.
BenefitsofUniversalTCellAssayStandardTERS™Kit-ComparABIlityofantigen-specifictestresultsoriginatingfromdifferenttimepoints,platformsorlaboratories-Quantitativeinformationonassayperformance-EasyproductionofTERS™-AllrequiredRNAreagentsavailableinonekit
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2.NaHCO3,Ba(OH)3,H2SO4
3.HCL,NaAlO2,NaHSO4
4.Ca(OH)2,Na2CO3,BaCO3
谢谢了
要原因
刚入这行，谢谢大家
通过解答，教会我，谢谢
D,K2SO4Na2CO3BaCL2NaNO3
请问下有无同学需要H37RA的？我是做EAE模型的，上个月购买了BDDifco公司的H37RA（货号），因为购买的时候只能整盒6支购买，但我们用不了那么多，所以想问问有无同学需要的，100mg/支，800元/支或用等价试剂交换。地址广州。有需要的请私信，谢谢！
（1）优级纯试剂 亦称保证试剂，为一级品，纯度高，杂质极少，主要用于精密分析和科学研究，常以GR表示。
（2）分析纯试剂 亦称分析试剂，为二级品，纯度略低于优级纯，杂质含量略高于优级纯，适用于重要分析和一般性研究工作，常以AR表示。
（3）化学纯试剂 为三级品，纯度较分析纯差，但高于实验试剂，适用于工厂、学校一般性的分析工作，常以CP表示。
（4）实验试剂 为四级品，纯度比化学纯差，但比工业品纯度高，主要用于一般化学实验，不能用于分析工作，常以 LR表示。
以上按试剂纯度的分类法已在我国通用。根据化学工业部颁布的“化学试剂包装及标志”的规定，化学试剂的不同等级分别用各种不同的颜色来标志，见表1。
表1 我国化学试剂的等级及标志
2.NaNO3FeCl3AgNO3
分别有什么现象？谢谢回答！
