Universal T Cell Assay Standard TERS™ Kit 4
Product Code: TERS-04
For the production of TCR-engineered reference samples for performance monitoring of T-cell assays.Amount: Sufficient for the transfection of 40 million PBMCs Purity: RNase free MHC Restriction: HLA-A*0201Peptide: YMDGTMSQVKit Content: TCR RNA α chain (35 µg), TCR RNA β chain (35 µg), GFP RNA (50 µg), Peptide (50µg)Delivery Format: Kit with 4 reagents and instructions for useApplication(s): Standardization of T cell assays, Performance monitoring of T cell assays Indication(s)/Topic(s): Control, Assay StandardDelivery Time: 3 weeksPlease note! An MHC Dextramer® (or equivalent multimer) is required to test every batch of TCR-engineered reference samples. Please order the corresponding Tyrosinase Dextramer™ separately!
Universal T Cell Assay Standard TERS™ Kit Generate MHC-class-I restricted T-cell receptor (TCR)-engineered reference samples containing defined numbers of functional, antigen-specific T cells for standardization and monitoring of:
- MHC-Multimer staining (e.g. Dextramer ®);
- intracellular cytokine detection (ICS);
- ELISPOT assays.
They represent an ideal tool for validating and monitoring assay performance within or across institutions. This new product may be used to standardize antigen-specific tests over time, between different platforms, or different laboratories. It may also be used to calibrate antigen-specific assays. Specificity TERS™ Kits can be used to monitor the performance of functional and non-functional T-cell assays irrespective of the specificity selected. They are run in the same way as usual assay standards, i.e. in parallel to other samples tested in a given assay run. Please note, that for functional testing involving T-cell stimulation by a peptide presented on a specific MHC molecule, the MHC of the used cell materials must match the MHC restriction of the TERS Kit as indicated. In the event that the peptide recognized by the TERS is the same as used for stimulating other samples in an assay run, an additional level of control (e.g. integrity of the specific peptide) is provided. If this is desired, you may wish to check if a Kit for producing TERS recognizing your specific peptide is available
The preparation of a TERS™ batch with this product requires peripheral blood mononuclear cells (PBMCs), which may be obtained from a human buffy coat, for example (not supplied). Additional reagents, an electroporator and a flow-cytometer are required during the production process.
Benefits of Universal T Cell Assay Standard TERS™ Kit- Comparability of antigen-specific test results originating from different time points, platforms or laboratories- Quantitative information on assay performance- Easy production of TERS™- All required RNA reagents available in one kit
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2.NaHCO3,Ba(OH)3,H2SO4
3.HCL,NaAlO2,NaHSO4
4.Ca(OH)2,Na2CO3,BaCO3
谢谢了
要原因
请问有什么方法检测细胞内活性氧更好,各位有什么经验,及相关试剂盒推荐一下。
A.H2SO4Na2SO4AgNO3BaCl2.
B.NaOHNa2CO3NaHSO4MgCl2
C.CaCl2NaNO3MgSO4BaCl2
D.HNO3KOHKClK2CO3
请问下有无同学需要H37RA的?我是做EAE模型的,上个月购买了BDDifco公司的H37RA(货号),因为购买的时候只能整盒6支购买,但我们用不了那么多,所以想问问有无同学需要的,100mg/支,800元/支或用等价试剂交换。地址广州。有需要的请私信,谢谢!
(1)优级纯试剂 亦称保证试剂,为一级品,纯度高,杂质极少,主要用于精密分析和科学研究,常以GR表示。
(2)分析纯试剂 亦称分析试剂,为二级品,纯度略低于优级纯,杂质含量略高于优级纯,适用于重要分析和一般性研究工作,常以AR表示。
(3)化学纯试剂 为三级品,纯度较分析纯差,但高于实验试剂,适用于工厂、学校一般性的分析工作,常以CP表示。
(4)实验试剂 为四级品,纯度比化学纯差,但比工业品纯度高,主要用于一般化学实验,不能用于分析工作,常以 LR表示。
以上按试剂纯度的分类法已在我国通用。根据化学工业部颁布的“化学试剂包装及标志”的规定,化学试剂的不同等级分别用各种不同的颜色来标志,见表1。
表1 我国化学试剂的等级及标志
bhclna2so4nano3na2co3
chclnaohna2co3nacl
dba(oh)2nahco3alcl3nahso4