PepMix™CollectionEBV(25ug/peptide)
ProductCode:PM-C-EBV-2
Collectionof14peptidepools(overlappingpeptidescans,15merswith11aaoverlap)through14immunodominantantigensofEpstein-Barrvirus(EBV).
Amount:14peptidepoolscontaining25µgperpeptide/tube
Purity:>70%(HPLC-MS)
DeliveryFormat:Freezedriedin14ambertypeglassvials
Application(s):T-cellassays,Immunemonitoring,AntigenspecificT-cellstimulation,T-cellexpansion,Cellularimmuneresponse,Patientstratification,Qualificationofimmuneresponses
Indication(s)/Topic(s):Infection,Cancer,Hodgkin"slymphoma,Burkitt"slymphoma,Nasopharyngealcarcinoma,Dermatomyositis,Systemiclupuserythematosus,Rheumatoidarthritis,Sjgren"ssyndrome,Multiplesclerosis
DeliveryTime:2-5days
PepMixes™PeptidePools
Theuseofcompleteprotein-spanningmixturesofoverlappingpeptides(PepMix™)isextremelyefficientforimmunostimulationofT-lymphocytesanddiagnosticapplications.InordertoestablishreliableandvalidatedT-cellassayssuchasELISPOT,appropriatepositiveandnegativecontrolsareessential.SelectionofsuchcontrolshelpstoconfirmproperfunctionalityoftheassayaswellasviABIlityofthecellsused.Inaddition,highqualitycontrolsaremostimportanttoestablishstandardizedassayprotocolsinclinicalsetups.Comparedtocommonlyusedcontrolslikephytohemagglutinin(PHA)andconcanavalinA(ConA)thatstimulateT-cellsaswellasothercelltypesorfulllengthantigens,syntheticpeptidepoolsoffertheadvantageofahighbatch-to-batchreproducibility,applicabilityofreliablechemicalandbiochemicalQC/QAmeasuresandshelfstabilitywhenstoredasfreezedriedaliquots.
BenefitsofPepMix™
-EquivalentorbetterstimulationofCD4+andCD8+T-cellresponsescomparedtowholeproteinantigens
-SimultaneousdetectionofCD4+andCD8+responsesinasinglesample
-ImprovedresponsesinstoredbloodandPBMcellscomparedtowholeproteinantigens
-Morereliableascontrolthanphytohemagglutinin(PHA)andconcanavalinA(ConA)
-High-batch-to-batchreproducibility
-Reliablechemicalandbiochemicalqualitycontrolandqualityassuranceforpeptidesynthesisandpeptidepoolgeneration
-Prolongedshelfstabilitywhenstoredfreezedried
PepMix™CollectionEBV(25ug/peptide)
SelectedReferences
"InVitroStudiesoftheImpactofMaribavironCMV-SpecificCellularImmuneResponses"
Stacheletal.,JClinVirol. (2015)-PMID:26780109
"InductionOfBroad-basedImmunityandProtectiveEfficacybySelf-amplifyingmRNAVaccinesEncodingInfluenzaVirusHemagglutinin"
Brazzolietal.,JournalofVirology (2015)-PMID:26468547
"TargetingofNucleoproteintoChemokineReceptorsbyDNAVaccinationResultsinIncreasedCD8+-mediatedCrossProtectionAgainstInfluenza"
Baranowskaetal.,Vaccine(2015)-PMID:26387432
"CharacteristicsofImmuneMemory10-15yearsAfterPrimaryHepatitisBVaccination"
Hummeletal.,Vaccine(2015)-PMID:n.a.
"TCellsSpecificforDifferentLatentandLyticViralProteinsEfficientlyControlEpstein-BarrVirusTransformedBCells"
Nowakowskaetal.,Cytotherapy(2015)-PMID:26276009
"High-densityPrecultureofPBMCsRestoresDefectiveSensitivityofCirculatingCD8TCellstoVirus-andTumor-derivedAntigens"
Wegneretal.,Blood(2015)-PMID:26024876
"MetabolicRegulationofHepatitisBImmunopathologybyMyeloid-derivedSuppressorCells"
Pallettetal.,NatureMedicine(2015)-PMID:25962123
ApplicationNotes
"DevelopingMulti-HIVAntigenSpecificT-CellsasaComponentofaCureStrategy"
Lametal.(2015)Fulltext
"Peptide-stimulatedExpansionofVirus-specificTcellsForPreventativeTreatmentAfterAllogeneicStemCellTransplantation"
Garyetal.(2015)Fulltext
PepMix™CollectionEBV(25ug/peptide)
| Application | AntigenspecificT-cellstimulation Immunemonitoring T-cellassays T-cellexpansion |
| ProteinName | Selectedproteins |
| Organism | Epstein-Barrvirus(HHV-4) |
| Indication/Topic | Autoimmunedisease Burkitt"slymphoma Hodgkin"slymphoma |
| Numberofpeptides | 2043 |
FurtherInformation:
| Amount | 1aliquotof14peptidepoolscontaining25µgperpeptide |
| Specifications | Peptidescan(15merswith11aaoverlap) |
| Documentation | ProtocolandDatasheet |
| Sequence | SeeDatasheetunderProduct Documentation |
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A、Na2CO3HClH2SO4NaNO3
B、K2CO3H2SO4HNO3BaCl2
C、HClHNO3AgNO3NaCl
D、NaOHFeCl3MgCl2BaCl2
（1）优级纯试剂 亦称保证试剂，为一级品，纯度高，杂质极少，主要用于精密分析和科学研究，常以GR表示。
（2）分析纯试剂 亦称分析试剂，为二级品，纯度略低于优级纯，杂质含量略高于优级纯，适用于重要分析和一般性研究工作，常以AR表示。
（3）化学纯试剂 为三级品，纯度较分析纯差，但高于实验试剂，适用于工厂、学校一般性的分析工作，常以CP表示。
（4）实验试剂 为四级品，纯度比化学纯差，但比工业品纯度高，主要用于一般化学实验，不能用于分析工作，常以 LR表示。
以上按试剂纯度的分类法已在我国通用。根据化学工业部颁布的“化学试剂包装及标志”的规定，化学试剂的不同等级分别用各种不同的颜色来标志，见表1。
表1 我国化学试剂的等级及标志
刚入这行，谢谢大家
