Aiko Konoshima_新闻_电影网_蚂蚁淘，【正品极速】生物医学科研用品轻松购|ebiomall -蚂蚁淘商城

商品列表技术文章相关问答

当前位置： > 首页 > 技术文章 >

Aiko Konoshima_新闻_电影网

来自 : 蚂蚁淘

08-15Buffer

CompoundMWFinalconcentration

200ml

Glycine75.070.1M

1.5g

1MMgCl₂10mM

2ml

KOHtopH9H₂Oto200ml

AceticAcid1M

CompoundMWFinalconcentration

50ml

AceticAcid98%(~17M)1M

3ml

H₂O

to50ml

Acrylamide/Bisacrylamide

CompoundMWFinalconcentration

500ml

Acrylamide71.0820%

100g

Bisacrylamide154.170.8%

H₂O

to500ml

AlkalineLysisBufferA

CompoundMWFinalconcentration

500ml

Glucose18050mM

4.5g

Tris12125mM

1.5g

EDTA0.25M10mM

20ml

H₂O

to450ml

AdjustpH8.0withHClH₂O,Autoclave,storeat4°C

to500ml

AlkalineLysisSolution1

CompoundMWFinalconcentration

100ml

Lysozyme0.2mg/ml

0.02g

AlkalineLysisBufferA

to100ml

AlkalineLysisSolution2

CompoundMWFinalconcentration

50ml

SDS20%1%

2.5ml

NaOH10M0.2M

1ml

H₂O

to50ml

AlkalineLysisSolution3

CompoundMWFinalconcentration

50ml

KAc983M

14.7g

AceticAcid100%11.5%

5.75ml

H₂O

to50ml

AmidoBlack

CompoundMWFinalconcentration

100ml

Amidoblack980.1%

0.1g

AceticAcid100%7.5%

7.5ml

Methanol20%(v/v)

20ml

H₂O

to100ml

AminoacidConcentrationinMedia

ConstituentFinalmg/lStock

mlstockfor1litermedium

Adeninesulfate202mg/ml10Uracil202mg/ml10L-tryptophan202mg/ml10L-histidine-HCl202mg/ml10L-arginine-HCl202mg/ml10L-methionine202mg/ml10L-tyrosine303mg/ml10L-leucine303mg/ml10L-isoleucine303mg/ml10L-lysine-HCl303mg/ml10L-phenylalanine505mg/ml10L-glutamicacid10010mg/ml10L-asparticacid10010mg/ml10L-valine15015mg/ml10L-threonine20020mg/ml10L-serine40040mg/ml10

3-Aminophtalhydrazide250mM

CompoundMWFinalconcentration

10ml

3-Aminophtalhydrazide177250mM

442mg

H₂O

to10ml

AmmoniummolyBDate4.2%

CompoundMWFinalconcentration

50ml

Ammoniummolybdatex4H₂O2364.2%

2.1g

H₂O

to30ml

HClfuming37%(~12M)4M

16.7ml

H₂O

to50ml

Ampicillin20mg/mlpH8.0

CompoundMWFinalconcentration

15ml

Ampicillin349.420mg/ml

300mg

H₂O

to10ml

AdjustpH8.0withNaOHH₂O,Filtersterilize

to15ml

Antibiotics

Antibioticsmg/mlonplatesActionResistanceMechanismAmpicillin50Inhibitscellwallsynthesisbyinhibitingformationofthepeptidoglycancrosslinkb-lactamase(286aa)issecretedintotheperiplasmandcleaveslactamantibioticChloramphenicol20Inhibitsproteinsynthesisbyinteractingwiththe50Sribosomalsubunitandinhibitingthepeptidyltransferasereaction.216aaproteincytoplasmicproteinwhichacetylateschloramphenicolKanamycinNeomycin30Inhibitstranslocationandelicitsmiscodingbybindingtothe30Sribosomalsubunit.256aaaminoglycosidephosphotransferasephosphorylateskanamycin,neomycin,etc.Tetracycline12InhibitsproteinsynthesisbypreventingbindingofaminoacyltRNAtotheribosomeAsite.428aamembraneproteincatalyzingeffluxoftetracyclin,probablybyacationsymportmechanism.

APS

CompoundMWFinalconcentration

10ml

Ammoniumpersulfate228.210%

H₂O,storeat4°C

to10ml

Ascorbate0.5MpH6.0

CompoundMWFinalconcentration

50ml

L-Ascorbicacid176.10.5M

4.4g

H₂O

to22ml

AdjustpH6.0withNaOH1MH₂O

to50ml

Asolectin(fattyacidfree)50mg/ml

CompoundMWFinalconcentration

50ml

Asolectin(acetontreated)50mg/ml

2.5g

Diethylether,storeat-20°C

to50ml

ATP200mM

CompoundMWFinalconcentration

10ml

Adenosine-5"-Triphosphate551.1200mM

1.102g

H₂O

to5ml

AdjustpH6.5withNaOH1MH₂O,storeat-20°C

to10ml

ATPRegeneratingSystem(ARS)40x

CompoundMWFinalconcentration

1ml

Phospoenolpyruvate(PEP)208.020mM

4.16mg

PyruvateKinase(PK)5U/ml0.1U/ml

20ml

H₂O,storeat4°C

to1ml

BALPProteaseInhibitorMix21x

CompoundMWFinalconcentration

10ml

Aprotinin1.5mg/ml0.486g/l

3.24ml

PepstatinA1mg/mlinDMSO6860.131g/l

1.3ml

Leupeptinx1/2H₂SO₄10.5mM4760.04mM

37.5ml

4-Aminobenzamidinex2HCl208190mM

396mg

H₂O

to10ml

Bis-Tris1MpH7.0

CompoundMWFinalconcentration

100ml

Bis-Tris209.21M

20.9g

H₂O

to50ml

AdjustpH7.0withHClH₂O

to100ml

Brij/DocpH8.0

CompoundMWFinalconcentration

100ml

EDTA0.25M1mM

0.4ml

Tris1MpH8.010mM

1ml

Na-Deoxycholate8%0.4%

5ml

Brij581%

H₂O

to90ml

Dissolveat37°C,adjustpH8.0H₂O,Filtersterilizeto100ml

BS³BufferpH7.5

CompoundMWFinalconcentration

1000ml

HEPES238.3100mM

23.83g

Dodecylmaltoside510.60.05%

0.5g

EDTA0.25M1mM

4ml

DTT15410mM

1.54g

H₂O

to900ml

AdjustpH7.5withNaOH10MH₂Oto1000ml

CdSO₄1M

CompoundMWFinalconcentration

1.5ml

CdSO₄208.51M

313mg

H₂O

to1.5ml

Chloroform

CompoundMWFinalconcentration

50ml

Chloroform

48ml

Isoamylalcohol4%

2ml

CIPBuffer4x

CompoundMWFinalconcentration

15ml

EDTA0.25MpH8.0200mM

12ml

Tris1MpH8.00.4mM

6ml

H₂O

3ml

Citrate1MpH5.O

CompoundMWFinalconcentration

50ml

CitratexH₂O2101M

10.5g

H₂O

to35ml

AdjustpH5.0withNaOH10MH₂Oto50ml

ColonyPCRBuffer10x

CompoundMWFinalconcentration

15ml

Tris1MpH6.8125mM

1.875ml

KCl74.50.5625M

0.629g

H₂O,Autoclave

to15ml

CoomassieBlueSolution

CompoundMWFinalconcentration

1000ml

CoomassieBlue(ServaBlau)0.2%

Aceticacid7.5%

75ml

Methanol50%

500ml

H₂O

to1000ml

CoumaricAcid40mM

CompoundMWFinalconcentration

10ml

p-Coumaricacid16440mM

66mg

H₂O

to10ml

CS/PBS

CompoundMWFinalconcentration

200ml

PBS,20x1x

10ml

Newborncalfserum10%

20ml

H₂O,Storefrozen200ml

CuSO₄1M

CompoundMWFinalconcentration

25ml

CuSO₄159.61M

H₂O

to25ml

DEPCtreatedH₂O

CompoundMWFinalconcentration

1000ml

H₂O

1000ml

Diethylpyrocarbonate(DECP)0.1%

1ml

LetsitatRTfor1h,Autoclave

DestainSolution

CompoundMWFinalconcentration

1000ml

Aceticacid7.5%

75ml

Ethanol10%

100ml

H₂O

to1000ml

Developer5xw/o

CompoundMWFinalconcentration

2000ml

Na₂CO₃10%

200g

H₂O

to2000ml

DialysisTubingforDNA

Product/ReagentVolumeTreatmentVisking20/32tubing6mCut30piecesof20cm2%NaHCO₃2lBrieflyboiltubing10mMEDTA2lBrieflyboiltubing10mMEDTA200mlStoretubingsubmergedH₂ORinsetubingbeforeuse

DNALoADIngBuffer6x

CompoundMWFinalconcentration

10ml

Bromophenolblue0.25%

25mg

XyleneXyanol0.25%

25mg

Sucrose40%

H₂O

to10ml

Dodecylmaltoside10%

CompoundMWFinalconcentration

50ml

Dodecylmaltoside510.610%

H₂O,Storeat4°C

to50ml

DTT1M

CompoundMWFinalconcentration

5ml

Dithiothreitol1541M

770mg

H₂O,Storeat4°C

to10ml

ECLSolution1

CompoundMWFinalconcentration

102ml

Tris-Cl200mMpH8.5200mM

100ml

3-Aminophtalhydrazine250mM2.5mM

1ml

Coumaricacid40mM,Filter0.4mM

1ml

ECLSolution2

CompoundMWFinalconcentration

100ml

H₂O

100ml

H₂O₂30%

60ml

EDTA(Na-Salt)0.25MpH8.0

CompoundMWFinalconcentration

100ml

EDTA(Na₂salt)3720.25M

9.3g

H₂O

to70ml

AdjustpH8.0with10MNaOHH₂O,Autoclave

to100ml

FeCl₃1M

CompoundMWFinalconcentration

1.5ml

FeCl₃x6H₂O2701M

406mg

H₂O

to1.5ml

Ferrozine100mM

CompoundMWFinalconcentration

1ml

Ferrozine492.5100mM

49.2ml

NaOH1M

2ml

H₂O

to1ml

Folin50%

CompoundMWFinalconcentration

50ml

FolinReagens50%

25ml

H₂O

to50ml

FPLCSolutionApH7.4

CompoundMWFinalconcentration

500ml

Tris121.1450mM

3.03g

Glycerol87%10%

57.5ml

Dodecylmaltoside10%0.1%

5ml

H₂O

to460ml

AdjustpH7.4with1MH₂SO₄H₂O

to500ml

FPLCSolutionBpH7.4

CompoundMWFinalconcentration

500ml

Imidazol68200mM

6.8g

Tris121.1450mM

3.03g

Glycerol87%10%

57.5ml

Dodecylmaltoside10%0.1%

5ml

H₂O

to460ml

AdjustpH7.4with1MH₂SO₄H₂O

to500ml

FPLCSolutionCpH7.4

CompoundMWFinalconcentration

500ml

K₂SO₄174.3200mM

17.4g

Tris121.1450mM

3.03g

Glycerol87%10%

57.5ml

Dodecylmaltoside10%0.1%

5ml

H₂O

to460ml

AdjustpH7.4with1MH₂SO₄H₂O

to500ml

FrenchPressBuffer(FPB)

CompoundMWFinalconcentration

500ml

Tris-SO41MpH7.450mM

25ml

Glycerol87%10%

57.5ml

H₂O,Autoclave

to500ml

Glutathione100mM

CompoundMWFinalconcentration

1.5ml

Glutathione(GSH)307.4100mM

46mg

H₂O,Storeat4°C

to1.5ml

Glycerol50%

CompoundMWFinalconcentration

50ml

Glycerol87%50%

28.75ml

H₂O,Autoclave

to50ml

HCl1M

CompoundMWFinalconcentration

50ml

HCl37%(~12M)1M

4.2ml

H₂O

to50ml

HIS-Stock2mg/ml

CompoundMWFinalconcentration

100ml

L-Histidine1552mg/ml

200mg

H₂O,Autoclave

to100ml

IPTG1M

CompoundMWFinalconcentration

5ml

Isopropyl-b,D-thiogalactoside238.31M

1.19g

H₂O,Filtersterilize

to5ml

Kanamycin10mg/ml

CompoundMWFinalconcentration

15ml

Kanamycin582.610mg/ml

150mg

H₂O,Filtersterilize

to15ml

KH₂PO₄0.4M

CompoundMWFinalconcentration

50ml

KH₂PO₄136.10.4M

2722mg

H₂O

to50ml

K-Na-Tartrate2%

CompoundMWFinalconcentration

50ml

K-Na-tartratex4H₂O2%

H₂O

to50ml

LaemmliElectrophoresisBuffer

CompoundMWFinalconcentration

10liters

Tris121.1425mM

30g

Glycine75192mM

144g

EDTA(TriplexII)2921mM

2.9g

SDS288.43.5mM

10g

H₂O

to10liters

LBMedia

CompoundMWFinalconcentration

1000ml

YeastExtract(BBL)0.5%

TrypticasePeptone(BBL)1%

10g

NaCl1%(0.2M)

10g

H₂O,Autoclave

to1000ml

LBPlates

CompoundMWFinalconcentration

1000ml

YeastExtract(BBL)0.5%

TrypitcasePeptone(BBL)1%

10g

NaCl0.2M

10g

Agar1.5%

15g

H₂O,Autoclave

to1000ml

LBLowSaltLiquid

CompoundMWFinalconcentration

1000ml

BactoYeastExtract0.5%

BactoPeptone1%

10g

NaCl0.1M

H₂O,Autoclave

to1000ml

LBLowSaltPlates

CompoundMWFinalconcentration

1000ml

BactoYeastExtract0.5%

BactoPeptone1%

10g

NaCl0.1M

Agar1.5%

15g

H₂O,Autoclave

to1000ml

LEU-Stock3mg/ml

CompoundMWFinalconcentration

50ml

L-Leucine3mg/ml

150mg

H₂O,Filtersterilize

to50ml

LiAc1M

CompoundMWFinalconcentration

100ml

LiAc-dihydrate1021M

10.2g

H₂O

to60ml

TE10x

10ml

H₂O,Autoclave

to100ml

LiCl1M

CompoundMWFinalconcentration

50ml

LiCl42.41M

2.12g

H₂O,Autoclave

to50ml

LowryComponent

CompoundMWFinalconcentration

200ml

Na₂CO₃1063%

NaOH400.1M

800mg

H₂O

to200ml

LYS-Stock3mg/ml

CompoundMWFinalconcentration

50ml

DL-Lysine182.63mg/ml

150mg

H₂O,Autoclave

to50ml

M17Media

CompoundMWFinalconcentration

1000ml

NeopeptoneDifco0.5%

Bacto-Soytone0.5%

BeefExtract0.5%

YeastExtract0.25%

2.5g

AscorbicAcid1762.8mM

0.5g

2-Glycerolphosphatex5H₂O,disodiumsaltNote:weused19g/luptoDec.20023060.1%

H₂O,Autoclave,ChilltoRT

to1000ml

Glucose50%1%

20ml

MgSO₄1M1mM

1ml

MalachitGreen1.25mM

CompoundMWFinalconcentration

50ml

MalachitGreen482.61.25mM

30mg

H₂O

to50ml

MES100mMpH6.4

CompoundMWFinalconcentration

250ml

MES195.2100mM

4.88g

H₂O

to200ml

AdjustpH6.4withNaOHH₂O,Autoclave

to250ml

MgSO₄1M

CompoundMWFinalconcentration

15ml

MgSO₄246.51M

3.70g

H₂O

to15ml

MRS

CompoundMWFinalconcentration1000mlTrypticasePeptone(BBL)1%10gBeefExtract(Difco)0.3%3gYeastExtract0.5%5gK₂HPO₄17411.5mM2gNH₄Acetate7739mM3gtri-Na-Citratex2H₂O29410.2mM3gMgSO₄1M2460.8mM0.8mlMnSO₄0.4M0.4mM1mlTween800.1%1mlAdjustpH6.3with5NHClH₂O,Autoclaveto1000mlGlucose50%(orLactose=MRSL)1%20mlN

Na-Acetate2.5MpH5.2

CompoundMWFinalconcentration

50ml

NaAcx3H₂O1362.5M

17g

H₂O

to35ml

AdjustpH5.2withAceticAcid

ca.10ml

H₂O,Autoclave

to50ml

Na-Citrate34%

CompoundMWFinalconcentration

50ml

Na-citratex2H₂O29434%

17g

H₂O

to50ml

NaCl5M

CompoundMWFinalconcentration

50ml

NaCl58.55M

14.6g

H₂O

to50ml

Na-Deoxycholate8%

CompoundMWFinalconcentration

30ml

Deoxiycholicacid(Nasalt)8%

H₂O

to20ml

AdjustpH8.4with10MNaOHH₂O,Filtersterilize

to30ml

NaOH10M

CompoundMWFinalconcentration

500ml

NaOH4010M

200g

H₂O

to500ml

NickTranslationBuffer4x

CompoundMWFinalconcentration

10ml

Tris-Cl1MpH7.2200mM

2ml

MgSO₄1M40mM

400ml

DTT1M0.4mM

4ml

H₂O

to10ml

NiSO₄1M

CompoundMWFinalconcentration

1.5ml

NiSO₄154.81M

232mg

H₂O

to1.5ml

N-Media

CompoundMWFinalconcentration

500ml

Na₂HPO₄x2H₂O17860mM

5.33g

BactoYeastExtract5%

2.5g

BactoPeptone10%

H₂O,Autoclave

to490ml

Glucose50%1%

10ml

NTARegenerationBuffer

CompoundMWFinalconcentration

15ml

Guanidine-HCl(GuHCL)95.56M

8.6g

AceticAcid98%(~17M)0.2M

175ml

H₂O

to15ml

NTB100x

CompoundMWFinalconcentration

10ml

Nitrotetrazoliumblue20mg/ml

200mg

Dimethylsulfoxide

5ml

H₂Oto10ml

NTP-Mix12.5mMeach

CompoundMWFinalconcentration

80ml

dATP100mM12.5mM

10ml

dGTP100mM12.5mM

10ml

dCTP100mM12.5mM

10ml

dTTP100mM12.5mM

10ml

H₂O

40ml

Oxidizer10x

CompoundMWFinalconcentration

500ml

K₂Cr₂O₇2941%

H₃PO₄0.1%

0.5ml

H₂O

to500ml

PBS20x

CompoundMWFinalconcentration

1000ml

Na₂HPO₄x2H₂O1780.16M

28.5g

NaH₂PO₄xH₂O1380.04M

5.52g

NaCl58.52.8M

164g

H₂O(pHshouldbe7)

to1000ml

PBSaulait

CompoundMWFinalconcentration

300ml

PBS20x1x

15ml

Defattedpowderedmilk(SanoLait,Coop)2%

Azide,20%stock(tostABIlize;inhibitslactoperoxidase!)0.02%

0.3ml

H₂O

to300ml

PBS-Tween

CompoundMWFinalconcentration

300ml

PBS20x1x

15ml

Tween200.2%

600ml

H₂O

to300ml

PEG400050%

CompoundMWFinalconcentration

100ml

Polyethyleneglycol400050%

50g

H₂O,Autoclave

to100ml

Phenol(basic)

CompoundMWFinalconcentration

50ml

Phenol,distilled

50ml

TEtosaturate~20ml8-Hydroxyquinoline0.1%

50mg

Mercaptoethanol0.2%(v/v)

100ml

Phenol(stabilized)

CompoundMWFinalconcentration

50ml

Phenol(stabilized)

50ml

Mercaptoethanol0.2%

100ml

Phenol:Chloroform:IsoamylAlcohol(25:24:1)

CompoundMWFinalconcentration

50ml

Phenol25%

25ml

Chloroform24%

24ml

Isoamylalcohol1%

1ml

PIMProteaseInhibitorMix

CompoundMWFinalconcentration

20ml

TLCK(Tosyl-Lysinechloromethylketone)369100mM

738mg

TPCK(Tosyl-Phenylalaninechloromethylketone)352100mM

704mg

p-Aminobenzamidin-HCl208100mM

416mg

o-Phenanthroline198100mM

396mg

PMSF(Phenylmethylsulfonylfluoride)174100mM

348mg

DMSO,Store2mlaliquotsat-20°C(keepsupto1yearorlonger)

to20ml

PISProteaseInhibitorMix

CompoundMWFinalconcentration

20ml

TLCK(Tosyl-Lysinechloromethylketone)369100mM

738mg

TPCK(Tosyl-Phenylalaninechloromethylketone)352100mM

704mg

p-Aminobenzamidin-HCl208100mM

416mg

PMSF(Phenylmethylsulfonylfluoride)174100mM

348mg

DMSO,Store2mlaliquotsat-20°C(keepsupto1yearorlonger)

to20ml

PLATE

CompoundMWFinalconcentration

50ml

PEG400040%

20g

LiAc1020.1M

0.51g

Tris-Cl1MpH7.510mM

500ml

EDTA0.25M1mM

200ml

H₂O,Autoclave

to50ml

Poly-DL-Lysine

CompoundMWFinalconcentration

5ml

Poly-DL-Lysine10mg/ml

50mg

H₂O,storeat-20°C

to5ml

ProteinCrackingBuffer

CompoundMWFinalconcentration

50ml

Tris1MpH7.525mM

1.25ml

NaCl58.4150mM

0.44g

Dithiothreitol1541mM

8mg

H₂O,Storeat4°C

to50ml

ProteinRunningGelBuffer¹/₂

CompoundMWFinalconcentration

500ml

Tris1MpH8.80.75M

375ml

SDS20%0.2%

5ml

EDTA0.25M4mM

8ml

H₂O

to500ml

ProteinSampleBuffer3x

CompoundMWFinalconcentration

20ml

Tris0.1MpH80.03M

6ml

Glycerol50%30%

12ml

SDS7.5%

1.5g

DTT(Dithiothreitol)0.15%

30mg

Bromphenolblue0.05%

10mg

H₂O

to20ml

ProteinStackingGelBuffer¹/₃

CompoundMWFinalconcentration

500ml

Tris1MpH6.80.17M

83ml

SDS20%0.13%

3.3ml

EDTA0.25M2.7mM

5.3ml

H₂O

to500ml

PS5x

CompoundMWFinalconcentration

1000ml

Na₂HPO₄x2H₂O1780.4M

71.2g

NaH₂PO₄xH₂O1380.1M

13.8g

NaCl58.50.5M

29.25g

H₂O

to1000ml

PS-Tween

CompoundMWFinalconcentration

300ml

PBS5x1x

60ml

Tween200.2%

600ml

H₂O

to300ml

PS-Tweenaulait

CompoundMWFinalconcentration

15ml

Defattedpowderedmilk(SanoLait,Coop)10%

1.5g

PS-Tween

to15ml

ReconstitutionDialysisBuffer(RDB)

CompoundMWFinalconcentration

500ml

Tris121.1450mM

3.03g

H₂O

to450ml

AdjustpH7.5with1MH₂SO₄H₂O

to500ml

RusconiMix

CompoundMWFinalconcentration

10ml

Lysozyme10mg/ml0.5mg/ml

500ml

EDTA0.25M25mM

1ml

Tris1MpH7.525mM

250ml

RNaseA10mg/ml0.1mg/ml

100ml

DNALoadingBuffer6x

1.5ml

H₂O

to10ml

SDLiquid

CompoundMWFinalconcentration

1000ml

YeastNitr.Basew/oaa0.67%

6.7g

Dextrose2%

20g

H₂O,Autoclave

to1000ml

SDPlates

CompoundMWFinalconcentration

1000ml

YeastNitr.Basew/oaa0.67%

6.7g

Dextrose2%

20g

Agar2%

20g

H₂O,Autoclave,cool,pour

to1000ml

SilverStain10x

CompoundMWFinalconcentration

1000ml

AgNO₃1702%

20g

H₂O,storeinabrownbottle

to1000ml

Sorbitol0.8M

CompoundMWFinalconcentration

250ml

Sorbitol182

36.43g

H₂O,Autoclave

to250ml

SSC20x

CompoundMWFinalconcentration

1000ml

NaCl58.443M

175g

Na₃-citrate294.10.3M

88.2g

H₂O

to450ml

AdjustpH7.0with1MHClH₂O

to500ml

STET

CompoundMWFinalconcentration

50ml

Sucrose(Saccharose)8%

TritonX-1005%

2.5ml

EDTA0.25M1mM

200ml

Tris-Cl1MpH8.050mM

2.5ml

Na-azide20%0.02%

50ml

H₂O

to50ml

Sucrose25%inTrispH8.0

CompoundMWFinalconcentration

100ml

Sucrose25%

25g

Tris1MpH8.050mM

5ml

H₂O,Autoclave

to100ml

SulfuricAcid1M

CompoundMWFinalconcentration

50ml

H₂O

40ml

H₂SO₄95-97%(~18M)1M

2.8ml

H₂O

to50ml

CompoundMWFinalconcentration

100ml

Tris-base1M10mM

1ml

EDTA0.25M1mM

0.4ml

AdjustpH8.0withHClH₂O,Autoclaveto100ml

TE10x

CompoundMWFinalconcentration

100ml

Tris-Cl1MpH8.0100mM

10ml

EDTA0.25M10mM

4ml

H₂O,Autoclave

to100ml

TEA100x

CompoundMWFinalconcentration

500ml

EDTA(Na₂salt)3720.1M

18.6g

Tris1214M

242g

H₂O

to400ml

Aceticacid100%11.4%

57ml

H₂O

to500ml

TES

CompoundMWFinalconcentration

100ml

Tris1MpH8.050mM

5ml

EDTA0.25M5mM

2ml

NaCl58.550mM

0.3g

H₂O,Autoclave

to100ml

TFB1

CompoundMWFinalconcentration

500ml

KAc98.230mM

1.473g

RbCl120.910mM

605mg

CaCl₂x2H₂O14710mM

735mg

MnCl₂125.850mM

3.145g

Glycerol87%15%

86ml

H₂O

to450ml

AdjustpH5.8with0.2MAcOHH₂O,Filtersterilize

to500ml

TFB2

CompoundMWFinalconcentration

500ml

MOPS209.310mM

1.047g

RbCl120.910mM

605mg

CaCl₂x2H₂O14775mM

5.51g

MnCl₂125.850mM

3.145g

Glycerol87%15%

86ml

H₂O

to450ml

AdjustpH6.5with0.2MKOHH₂O,Filtersterilize

to500ml

TricineAnodeBuffer(+)

CompoundMWFinalconcentration

1000ml

Tris121.140.2M

24.23g

H₂O

to900ml

AdjustpH8.9withHClH₂O

to1000ml

TricineCathodeBuffer(-)

CompoundMWFinalconcentration

1000ml

Tris121.140.1M

12.11g

Tricine179.170.1M

17.92g

SDS20%0.1%

5ml

H₂Oto1000ml

TricineGelAcrylamide

CompoundMWFinalconcentration

250ml

Acrylamide71.0848%

120g

Methylene-Bisacrylamide154.171.5%

3.75g

H₂O,avoidsunlightto250ml

TricineGelBuffer

CompoundMWFinalconcentration

250ml

Tris121.143M

90.86g

H₂O

to200ml

AdjustpH8.45withHClSDS20%0.3%

3.75ml

H₂Oto250ml

Tris1MpHX

CompoundMWFinalconcentration

100ml

Tris1211M

12.1g

H₂O

to80ml

AdjustpHXwithHClH₂O,Autoclave

to100ml

Tris-SO₄1MpH7.4

CompoundMWFinalconcentration

100ml

Tris1211M

12.1g

H₂O

to90ml

AdjustpH7.4withH₂SO₄H₂O,Autoclave

to100ml

TRP-Stock2mg/ml

CompoundMWFinalconcentration

40ml

L-Tryptophan2042mg/ml

80mg

H₂O,Filtersterilize

to40ml

storeat4°Cindark

TSS

CompoundMWFinalconcentration

50ml

Polyethyleneglycol6000600010%

DMSO5%

2.5ml

MgSO₄246.520mM

1ml

LB,Autoclave

to50ml

URA-Stock2mg/ml

CompoundMWFinalconcentration

100ml

Uracil1122mg/ml

200mg

H₂O,Autoclave

to100ml

Vanadate20mM

CompoundMWFinalconcentration

5ml

Na₃VO₄x5H₂O27420mM

27.4mg

H₂O,storeat-20°C

to5ml

WesternTransferBuffer10x

CompoundMWFinalconcentration

2000ml

Tris121.140.3M

72.7g

Glycine752M

300g

SDS20%2880.2%

H₂O

to2000ml

XP100x

CompoundMWFinalconcentration

10ml

5-Bromo-4-chloro-indoxylphosphate10mg/ml

100mg

Dimethylsulfoxide

to10ml

YeastLysisBuffer

CompoundMWFinalconcentration

50ml

TritonX-1002%

1ml

SDS20%1%

2.5ml

NaCl58.5100mM

0.3g

Tris-Cl1MpH8.010mM

500ml

EDTA0.25M1mM

200ml

H₂O

to50ml

YPDLiquid

CompoundMWFinalconcentration

1000ml

BactoYeastExtract1%

10g

BactoPeptone2%

20g

Dextrose2%

20g

H₂O,Autoclave

to1000ml

YPDPlates

CompoundMWFinalconcentration

1000ml

BactoYeastExtract1%

10g

BactoPeptone2%

20g

Dextrose2%

20g

Agar2%

20g

H₂O,Autoclave,cool,pour

to1000ml

YPGLiquid

CompoundMWFinalconcentration

1000ml

BactoYeastExtract1%

10g

BactoPeptone2%

20g

Galactose2%

20g

H₂O,Autoclave

to1000ml

YPGPlates

CompoundMWFinalconcentration

1000ml

BactoYeastExtract1%

10g

BactoPeptone2%

20g

Galactose2%

20g

Agar2%

20g

H₂O,Autoclave,cool,pour

to1000ml

ZnSO₄1M

CompoundMWFinalconcentration

1.5ml

ZnSO₄x7H₂O287.51M

431mg

H₂O

to1.5ml

免责声明本文仅代表作者个人观点，与本网无关。其创作性以及文中陈述文字和内容未经本站证实，对本文以及其中全部或者部分内容、文字的真实性、完整性、及时性本站不做任何保证或承诺，请读者仅作参考，并请自行核实相关内容。

发布于： 2021-08-16 阅读（240）

下一篇 : 如何与「心机」boy 和睦相处？实验室离心机安全必备知识

▍ 相关分类

缓冲液

酸碱缓冲液上样缓冲液电泳缓冲液其它缓冲液

生物分子

氨基酸核苷酸脂类固醇类糖类蛋白交联剂储存液反应底物小分子受体转运体激素脂质体

染色剂

荧光染料其他生物染料

其他

酸碱盐醇酮酚胺酯微球磁珠琼脂糖发光剂去垢剂多聚物表面活性剂其他试剂单体

标准品

微球标准品荧光染料标准品生化标准品

脂质代谢研究

▍ 相关文章

more+

适用于尿17羟、17酮检查的防腐剂是A.二甲苯B.甲醛C.浓盐酸D.浓硫... Wireshark · Get Involved IMMUNOMEDICS, INC 产品平台分析 GII 无菌室标准化规程及验收规范_ 高性价比ELISA试剂盒,现货!还有代检测服... 来自爱必信生物微博中检所对照品【CAS:458377|姜黄素】生产方法_中检所对照品,CAS... طرز تهیه الکل گندم 奎克化学QUAKERCHEMICAL.PDF Aiko Konoshima_新闻_电影网肝硬化实验动物的模型建立与研究现状在滤膜上进行细菌DNA的杂交实验资讯 CMCC(B)26003中检所菌种 CMCC(B)26003 金黄色葡萄球菌260035a28

▍ 相关问答

more+

标准品和对照品,这俩有啥不同？知识概念实验与分析

请教原料药的原料质量标准问题原料药/兽药蒲公英制药技术的传播...

...标准品”和“对照品”这两个名称哪个更规范?谢药理及临床试验...

二类医疗器械临床检验仪器产品注册申报材料要求及常见错误

有关于标准品,校准品及质控品的区别

临床实验室生化标准品的英文名缩写是什么,如血脂标准品 55问答网

【讨论】标准品和对照品有什么区别?期待您的参与!_化学试剂仪器社区_仪器...

对于没有标准品的样品怎样确定含量?质量检验蒲公英制药技术...

【求助】想问问标准品的处理!_化学试剂_基础知识专区_仪器论坛

生化标准品_标准品_生化试剂_知道_生物信息网

生化分析仪50问

【共享】对照品和标准品的概念制剂技术讨论版论坛