
Catalog number | MP061M |
Product type | ELISA |
Quantity | 96 Tests (12x8 breakable strip wells) |
Sample volume | 10 µl/well |
Species | Human |
Storage and Stability | Product should be stored at 2-8 °C. |
Precautions | For research use only. Not for use in diagnostic procedures. |
References | 1. Davidkin I; Valle M; Julkunen I. Persistence of anti-mumps virus antibodies after a two-dose MMR vaccination. A nine-year follow-up.Vaccine 1995;13(16):1617-22. 2. Chomel JJ; Robin Y; Durdilly R; Thouvenot D; Langlois M; Aymard M.Rapid direct diagnosis of mumps meningitis by ELISA capture technique. J Virol Methods 1997;68(1):97-104. 3. Nigro G; Nanni F; Midulla M. Determination of vaccine-induced and naturally acquired class-specific mumps antibodies by two indirect enzyme-linked immunosorbent assays. J Virol Methods 1986;13(2):91-106. 4. Harmsen T; Jongerius MC; van der Zwan CW; Plantinga AD; Kraaijeveld CA; Berbers GA. Comparison of a neutralization enzyme immunoassay and an enzyme-linked immunosorbent assay for evaluation of immune status of children vaccinated for mumps. J Clin Microbiol 1992;30(8):2139-44. 5. Novotn´y J. Properties and use of mumps viral antigen for detection of specific IgG and IgM antibodies in enzyme-linked immunosorbent assay. Acta Virol 1990;34(6):574-7. 6. Johnson CE; Kumar ML; Whitwell JK; Staehle BO; Rome LP; Dinakar C; Hurni W; Nalin DR. Antibody persistence after primary measles-mumps-rubella vaccine and response to a second dose given at four to six vs. eleven to thirteen years. Pediatr Infect Dis J 1996; 15(8):687-92. |
Mumps IgM ELISA Package InsertType : PDF
Mumps IgG ELISA

Rubella IgG ELISA

Rubella IgM ELISA

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第一,血清成分复杂,最好用密理博的Montage Albumin Deplete Kit去除血清里50%的白蛋白
第二,磷酸化蛋白提取蛋白的时候,最好加入原钒酸钠,和磷酸酶抑制剂,防止蛋白脱磷酸化。
第三,不要用牛奶做封闭剂,用BSA。因为牛奶中含有磷酸化酪蛋白,防止非特异带。
第四,如果条带杂带多,而且,条带弱,可以选择millipore的signal boost,增加条带特异性,而且,增强条带的亮度。
转膜后就要区分与胶接触的一面与另一面了。话说楼主好奇的话可以跑蛋白的试试看哈。比如目的蛋白的胶用光滑面贴着胶,内参蛋白用粗糙面贴着胶一起转膜试试转膜结果看看呢。
1. 使用预染 Marker,不过分子量不是特别准;
2. 所使用的 Marker 条带与待测蛋白质带有相同的抗原表位,比如都带有 His 融合标签;
3. 可以使用普通的 Marker 转膜使用 丽春红 等染色,然后在膜上标记出 Marker 各条带的位置。
考马斯亮兰染液也可以重复用。新配的染液10分钟即可,重复3次后要染30分钟。
一抗二抗可以重复利用,但是注意要在5%milk中加入0.2% sodium azide ,并且用完以后放入4度保存,我的经验重复使用4-5次是肯定没有问题的.如果保存不当,就会污染微生物,只能丢弃.

