CLIA Kit for Chemokine C-X3-C-Motif Ligand 1 (CX3CL1)
NTN; ABCD3; C3Xkine; CXC3; CXC3C; NTT; SCYD1; ABCD3; FKN; Neurotactin; Fractalkine; Small Inducible Cytokine Subfamily D(Cys-X3-Cys)Member 1
- Product No.SCA040Mu
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Caprine
- Ovine
- Equine
- Bovine
- Porcine
- Gallus
- Canine
- Others
- Multi-species
- Pan-species
- Test MethodDouble-antibody Sandwich
- Assay Length2h, 40min
- Detection Range3.43-2,500pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 1.51pg/mL.
- Sample TypeSerum, plasma and other biological fluids
- DownloadInstruction Manual
- UOM48T96T96T*596T*1096T*100
- FOBUS$ 605 For more details, please contact local distributors!US$ 864 For more details, please contact local distributors!US$ 3888 For more details, please contact local distributors!US$ 7344 For more details, please contact local distributors!US$ 60480 For more details, please contact local distributors!
Packages (Simulation)- Packages (Simulation)
- Results demonstration
- ISO9001: 2008, ISO13485: 2003 Registered
Specificity of the CLIA Kit for Chemokine C-X3-C-Motif Ligand 1 (CX3CL1)
This assay has high sensitivity and excellent specificity for detection of Chemokine C-X3-C-Motif Ligand 1 (CX3CL1).No significant cross-reactivity or interference between Chemokine C-X3-C-Motif Ligand 1 (CX3CL1) and analogues was observed.
Recovery of the CLIA Kit for Chemokine C-X3-C-Motif Ligand 1 (CX3CL1)
Matrices listed below were spiked with certain level of recombinant Chemokine C-X3-C-Motif Ligand 1 (CX3CL1) and the recovery rates were calculated by comparing the measured value to the expected amount of Chemokine C-X3-C-Motif Ligand 1 (CX3CL1) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 86-101 | 97 |
| EDTA plasma(n=5) | 79-93 | 86 |
| heparin plasma(n=5) | 85-105 | 92 |
Precision of the CLIA Kit for Chemokine C-X3-C-Motif Ligand 1 (CX3CL1)
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Chemokine C-X3-C-Motif Ligand 1 (CX3CL1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Chemokine C-X3-C-Motif Ligand 1 (CX3CL1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10%>Inter-Assay: CV<12%>
Linearity of the CLIA Kit for Chemokine C-X3-C-Motif Ligand 1 (CX3CL1)
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Chemokine C-X3-C-Motif Ligand 1 (CX3CL1) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 88-97% | 91-98% | 91-105% | 90-98% |
| EDTA plasma(n=5) | 80-89% | 79-93% | 86-93% | 81-93% |
| heparin plasma(n=5) | 94-102% | 85-99% | 85-95% | 93-103% |
Stability of the CLIA Kit for Chemokine C-X3-C-Motif Ligand 1 (CX3CL1)
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary of the CLIA Kit for Chemokine C-X3-C-Motif Ligand 1 (CX3CL1)
1. Prepare all reagents, samples and standards;2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;4. Aspirate and wash 3 times;5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;6. Aspirate and wash 5 times;7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;8. Read RLU value immediately.
Test principle of the CLIA Kit for Chemokine C-X3-C-Motif Ligand 1 (CX3CL1)
The microplate provided in this kit has been pre-coated with an antibody specific to Chemokine C-X3-C-Motif Ligand 1 (CX3CL1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Chemokine C-X3-C-Motif Ligand 1 (CX3CL1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Chemokine C-X3-C-Motif Ligand 1 (CX3CL1) level in the sample or standard.;
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Related products
| Catalog No. | Organism species: Mus musculus (Mouse) | Applications (RESEARCH USE ONLY!) |
| RPA040Mu02 | Recombinant Chemokine C-X3-C-Motif Ligand 1 (CX3CL1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| EPA040Mu61 | Eukaryotic Chemokine C-X3-C-Motif Ligand 1 (CX3CL1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA040Mu01 | Recombinant Chemokine C-X3-C-Motif Ligand 1 (CX3CL1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA040Mu01 | Polyclonal Antibody to Chemokine C-X3-C-Motif Ligand 1 (CX3CL1) | WB; IHC; ICC; IP. |
| PAA040Mu02 | Polyclonal Antibody to Chemokine C-X3-C-Motif Ligand 1 (CX3CL1) | WB; IHC; ICC; IP. |
| SEA040Mu | ELISA Kit for Chemokine C-X3-C-Motif Ligand 1 (CX3CL1) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCA040Mu | CLIA Kit for Chemokine C-X3-C-Motif Ligand 1 (CX3CL1) | Chemiluminescent immunoassay for Antigen Detection. |
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在进行RT反应之前，应考虑以下几个方面：
1、RNA
成功的cDNA合成来自高质量的RNA，高质量的RNA至少应保证全长并且不含逆转录酶的抑制剂，如EDTA或SDS。在提取RNA的过程中，要特别防止RNase的污染，同时在逆转录反应中经常加入RNase抑制剂以增加cDNA合成的长度和产量。RNase抑制剂要在第一链cDNA合成反应中，在缓冲液和还原剂（如DTT）存在的条件下加入，因为cDNA合成前的过程会使抑制剂变性，从而释放结合的可以降解RNA的RNase。蛋白RNase抑制剂仅防止RNaseA，B，C对RNA的降解，并不能防止皮肤上的RNase，因此尽管使用了这些抑制剂，也要小心不要从手指上引入RNase，实验过程中经常更换新手套。
2、引物的选择
OligodT
选择OligodT时，要求RNA必须有PolyA，所以真核生物的mRNA都适用。适合长链甚至全长mRNA的RT，所以对RNA样品的质量要求较高，最好不要有明显的DNA污染、RNA降解和RNA断裂。假如想探索新的mRNA进行RT反应，建议推荐使用OligodT引物。使用OligodT引物要比随机引物和特异性引物的稳定性要好。
随机引物
适合各种RNA的RT，尤其适合模板丰度很低的情况（比如某个gene表达量很低）。选择随机引物时，第一链cDNA合成反应中就是以所有的RNA为模板，然后进行PCR反应时设计引物进行特异性扩增。同时要注意随机引物的量和总RNA量之间的关系，一般建议每5μg总RNA的随机引物的用量为50ng，如果每5μg总RNA的随机引物的用量超过250ng，可能会导致小片段产物（<500bp）的增加和长片断、全长产物产物的降低。
特异性引物
特异性引物只能用你设计引物时的下游引物做RT，引物设计质量影响RT的结果，而且不同引物退火温度本来就不相同，所以按照说明书按照一个温度做不是最佳选择，一般不推荐。向左转|向右转
只要是细胞，就得走向衰老，现代研究表明，细胞的衰老和端粒的变短有关系。端粒就是DNA，随着细胞分裂次数的增多，端粒在不断变短，端粒酶就是组织端粒变短的。然而正常细胞中，端粒酶的活性受抑制，端粒酶是怎么染端粒变长的呢？实际上端粒酶是由RNA组成的，可以根据碱基互补配对逆转形成DNA，使得变短的端粒变长。所以说端粒酶可以逆转录形成端粒，使端粒不减短！
多谢……
不过。。。。我时常用那种过期一两年的。。。。也能反转出来。只要你保存得当就可以了。一般是-20以下保存。
而且保真性要高一点的，因为今后要用来做表达
