CLIA Kit for Chemokine C-X3-C-Motif Ligand 1 (CX3CL1)
NTN; ABCD3; C3Xkine; CXC3; CXC3C; NTT; SCYD1; ABCD3; FKN; Neurotactin; Fractalkine; Small Inducible Cytokine Subfamily D(Cys-X3-Cys)Member 1
- Product No.SCA040Mu
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- All
- Human
- Mouse
- Rat
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- Rabbit
- Simian
- Caprine
- Ovine
- Equine
- Bovine
- Porcine
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- Canine
- Others
- Multi-species
- Pan-species
- Test MethodDouble-antibody Sandwich
- Assay Length2h, 40min
- Detection Range3.43-2,500pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 1.51pg/mL.
- Sample TypeSerum, plasma and other biological fluids
- DownloadInstruction Manual
- UOM48T96T96T*596T*1096T*100
- FOBUS$ 605 For more details, please contact local distributors!US$ 864 For more details, please contact local distributors!US$ 3888 For more details, please contact local distributors!US$ 7344 For more details, please contact local distributors!US$ 60480 For more details, please contact local distributors!
Packages (Simulation)- Packages (Simulation)
- Results demonstration
- ISO9001: 2008, ISO13485: 2003 Registered
Specificity of the CLIA Kit for Chemokine C-X3-C-Motif Ligand 1 (CX3CL1)
This assay has high sensitivity and excellent specificity for detection of Chemokine C-X3-C-Motif Ligand 1 (CX3CL1).No significant cross-reactivity or interference between Chemokine C-X3-C-Motif Ligand 1 (CX3CL1) and analogues was observed.
Recovery of the CLIA Kit for Chemokine C-X3-C-Motif Ligand 1 (CX3CL1)
Matrices listed below were spiked with certain level of recombinant Chemokine C-X3-C-Motif Ligand 1 (CX3CL1) and the recovery rates were calculated by comparing the measured value to the expected amount of Chemokine C-X3-C-Motif Ligand 1 (CX3CL1) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 86-101 | 97 |
| EDTA plasma(n=5) | 79-93 | 86 |
| heparin plasma(n=5) | 85-105 | 92 |
Precision of the CLIA Kit for Chemokine C-X3-C-Motif Ligand 1 (CX3CL1)
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Chemokine C-X3-C-Motif Ligand 1 (CX3CL1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Chemokine C-X3-C-Motif Ligand 1 (CX3CL1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10%>Inter-Assay: CV<12%>
Linearity of the CLIA Kit for Chemokine C-X3-C-Motif Ligand 1 (CX3CL1)
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Chemokine C-X3-C-Motif Ligand 1 (CX3CL1) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 88-97% | 91-98% | 91-105% | 90-98% |
| EDTA plasma(n=5) | 80-89% | 79-93% | 86-93% | 81-93% |
| heparin plasma(n=5) | 94-102% | 85-99% | 85-95% | 93-103% |
Stability of the CLIA Kit for Chemokine C-X3-C-Motif Ligand 1 (CX3CL1)
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary of the CLIA Kit for Chemokine C-X3-C-Motif Ligand 1 (CX3CL1)
1. Prepare all reagents, samples and standards;2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;4. Aspirate and wash 3 times;5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;6. Aspirate and wash 5 times;7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;8. Read RLU value immediately.
Test principle of the CLIA Kit for Chemokine C-X3-C-Motif Ligand 1 (CX3CL1)
The microplate provided in this kit has been pre-coated with an antibody specific to Chemokine C-X3-C-Motif Ligand 1 (CX3CL1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Chemokine C-X3-C-Motif Ligand 1 (CX3CL1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Chemokine C-X3-C-Motif Ligand 1 (CX3CL1) level in the sample or standard.;
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Related products
| Catalog No. | Organism species: Mus musculus (Mouse) | Applications (RESEARCH USE ONLY!) |
| RPA040Mu02 | Recombinant Chemokine C-X3-C-Motif Ligand 1 (CX3CL1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| EPA040Mu61 | Eukaryotic Chemokine C-X3-C-Motif Ligand 1 (CX3CL1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA040Mu01 | Recombinant Chemokine C-X3-C-Motif Ligand 1 (CX3CL1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA040Mu01 | Polyclonal Antibody to Chemokine C-X3-C-Motif Ligand 1 (CX3CL1) | WB; IHC; ICC; IP. |
| PAA040Mu02 | Polyclonal Antibody to Chemokine C-X3-C-Motif Ligand 1 (CX3CL1) | WB; IHC; ICC; IP. |
| SEA040Mu | ELISA Kit for Chemokine C-X3-C-Motif Ligand 1 (CX3CL1) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCA040Mu | CLIA Kit for Chemokine C-X3-C-Motif Ligand 1 (CX3CL1) | Chemiluminescent immunoassay for Antigen Detection. |
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没有序列识别的功能，他就是简单的催化相邻DNA链的5-P末端和3'-OH末端以磷酸二酯键结合的反应，需ATP作辅酶。 不仅可以催化粘性末端之间或平滑末端之间的DNA的连接，也催化DNA与RNA之间以及少数RNA之间的连接。
反转录酶（Reversetranscripatase）是以RNA为模板指导三磷酸脱氧核苷酸合成互补DNA（cDNA）的酶。哺乳类C型病毒的反转录酶和鼠类B型病毒的反转录酶都是一条多肽链。鸟类RNA病毒的反转录酶则由两上亚基结构。真核生物中也都分离出具有不同结构的反转录酶。
不过。。。。我时常用那种过期一两年的。。。。也能反转出来。只要你保存得当就可以了。一般是-20以下保存。
