
Description
Product Highlights
- Fast – streamlined protocol for consistent recovery of genomic DNA in as little as 20 minutes
- High-performance – extraction of high-purity DNA, ideal for use in all downstream applications
- High-yield –efficient sample lysis and column-binding for high-yield recovery of genomic DNA from limiting amounts of sample
- Flexible – reliable extraction of genomic DNA from a very broad range of human, animal and microbial samples
Product Description
By combining Proteinase K lysis with the speed and convenience of silica membrane purification, ISOLATE II Genomic DNA Kit provides a fast method for the purification of high-quality genomic DNA from a variety of starting materials.
By combining Proteinase K lysis with the speed and ease-of-use of silica-membrane purification, the ISOLATE II Genomic DNA Kit provides a fast method for the purification of high-quality genomic DNA from a variety of starting materials, such as mouse or rat tails, bacteria, yeast, dried blood spots, genomic/viral DNA from blood, hair follicles, paraffin-embedded issue (FFPE), insects, dental swabs, buccal swabs, stool viruses (e.g. CMV), Mycobacterium tuberculosis or Legionella pneumophila in sputum or bronchoalveolar lavage, EHEC bacteria in food, bacterial DNA (e.g. Chlamydia trachomatis) from cultures, biological fluids or clinical specimens, bacterial DNA (e.g. Borrelia burgordferi) and viral DNA (e.g. CMV) from urine.
ISOLATE II Genomic DNA Kit has been designed to deliver optimal performance in qPCR in tandem with SensiFAST Real-Time PCR Kits and in end-point PCR with MyTaq DNA Polymerase.
Applications
- qPCR
- End-point PCR
- Southern, dot and slot blotting
- Genotyping
- Restriction digestion
- Next Generation Sequencing
- Bisulfite conversion/methylation analysis

Nucleic Acid Isolation Guide
Download the ISOLATE II Guide with detailed product descriptions and performance data to help you choose the best product for your researchISOLATE II Selection Chart
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希望知道如何购买的朋友告诉一下,谢谢。
一般来说,试剂盒曲线上的最小浓度是比较准确的,低于这个值因为与曲线是个“S"型结构有关,所以结果是有偏差的,是一种估算。再加上实验误差,所以达不到理想的效果。建议选择试剂盒时,应该看曲线上最小的浓度值,而不是试剂盒上写的灵敏度。
虽然酶活性调节ELISA方法的灵敏度目前并不十分理想,但在酶活性放大ELISA中,检测的灵敏度远比RIA高。根据质量作用定律。即免疫反应所形成的免疫复合物量与反应物浓度成正比。推测所检测的待测物分子数为1。已知1个摩尔浓度含6.02×1023个分子,那么理论推测酶活性放大ELISA方法的最低检测限可达1.7×10-24mol/L。虽然在实际应用中由于反应条件和试剂纯度以及仪器精度等因素的影响,往往达不到这个水平(大于104个分子),但表明ELISA在灵敏度方面的改进潜力是很大的。
我使用的是北京北方生物技术研究所提供的KIT.盒子里面有睾酮标准品,兔抗睾酮抗体,带碘标记的睾酮,和驴抗兔免疫分离剂。实验过后我查了一下生物化学书,发现睾酮这种激素的化学本质非蛋白质,而是缁醇。这样问题就出来了:据我所知,产生免疫反应的是蛋白质,然而性类固醇激素的化学本质是缁醇。请问各位,缁醇也能产生免疫反应吗?

