As part of the Signal-Seeker™ product line, ubiquitination control beads (CUB02) have been developed as highly specific negative control beads for Cytoskeleton"s ubiquitination affinity bead (UBA01-Beads) products. These control beads are far superior to beads alone, because it has mutated versions of the UBDs covalently attached to the control bead matrix. This control is particularly important for ubiquitin IPs as the unmodified version of target proteins often bind non-specifically to all available ubiquitin enrichment affinity matrices. A comprehensive Signal-Seeker™ Ubiquitin Detection Kit (BK161), which includes ubiquitin control beads (CUB02), is available and is recommended for first time users.
Validation Data: Ubiquitination Control Beads White Paper
Each lot of affinity-bead is quality controlled to provide high batch to batch consistency, see COA documents.
Application 1: Utilization of IgG control beads
Figure 1: Swiss 3T3 cells were pre-treated with MG-132, then either untreated or treated with CNF1 (CN04) for 3 hours prior to lysis with BlastR buffer. The BK161 kit was utilized to perform the IP on 300 μg of lysate per condition. Input: 3 μg input untreated lysate, Ubiquitin Affinity beads (UBA01) plus untreated lysate, UBA01 plus CN04 treated lysate, ubiquitin control beads (CUB02) plus untreated lysate, and CUB02 plus CN04 treated lysate. Samples were analyzed for Rac1 ubiquitination using an anti-Rac1 antibody.
Amount:Each package contains enough ubiquitin control beads for 10 reactions.
For more information contact: signalseeker@cytoskeleton.com
Associated Products:
Signal-Seeker™ Ubiquitination Detection Kit (Cat. # BK161)
Signal-Seeker™ Ubiquitin Affinity Beads (Cat.# UBA01-beads)
Signal-Seeker™: BlastR™ Rapid Lysate Prep Kit (Cat. # BLR01)
Signal-Seeker™: PTMtrue™ Ubiquitin Antibody (Cat.# AUB01)
For the most recent publications citing this and other Signal-Seeker™ products, see our Signal-Seeker™ Validation Data Page click here
Visit our Signal-Seeker™ Tech Tips and FAQs page for technical tips and frequently asked questions regarding this and other Signal-Seeker™ products click here If you have any questions concerning this product, please contact our Technical Service department at tservice@cytoskeleton.com
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就是蛋白质分子的小片断
是氨基酸形成的
求助各位前辈，我最近在合成的化合物水溶性很好，非常好，以至于可以随便溶解在水里，它的六氟磷酸盐也可以随意溶解在水里（大于50uM），细胞成像实验显示它根本进不去细胞，求问有没有啥方法包裹一下让它进去？我搜了一下文献，感觉多数是把脂溶性特别好的东西包裹一下弄进去的，也许是搜索姿势不对没找到我需要的答案，**点拨啊！！！
如题，之前没做过药代，老师给了一个600+Da的五肽，想测下药代动参数，看文献推荐上述两种方法，但是不知道选哪种更好，lcms前处理会不会影响小肽。
有机的是有机化合物的简称,它指的是含碳化合物.
但是,有四大类常见物质一般不作为有机物处理：
1、碳的氧化物,如CO和CO2.
2、碳酸及其盐,如CaCO3.
3、金属碳化物,如CaC2.
4、拟卤素及其化合物,如(CN)2与KSCN.
水的化学式为H2O,它不含有碳元素,故不是有机物.
但若所描述的水不是化学意义的水,而是自然界存在的天然水,那么,水中会溶有一定量的有机物.
