Product Specifications: Item # 1004-F: Fluorescene rgp160 HIV-1 IIIB
Mass/vial: At least 50µg rgp160
Diluent: 10mM Tris, pH 8.0
Stabilizer: None
Preservative: None
Storage: -75°C
F/P Ratio: 2 - 4
Stability: At least 6 months at -75°C
Minimum Dilution: 1 : 50
Applications: Dot Blots, FACS, Immunohistocytology, Immunoflorecence
Description: Baculovirus derived rgp160 (HIV-1 IIIB) conjugated to FITC.
Purification: Fluorescene conjugated rgp160 was purified by gel exclusion chromatography and UF concentrated.
Specificity: This fluorescene conjugated rgp160 binds to murine monoclonal antibodies of defined epitope specificity and immobilized CD4 in Dot Blot assays.
Biological Activity: This fluorescene conjugated gp160 binds to CD4 bearing cells in FACS.
Application and Instruction for use: Recommended dilutions for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. Direct Immunofluorescent staining of CD4 bearing cells may be performed in 1 : 50 dilution range. Dot Blot assays with purified anti-gp160 monoclonal/polyclonal antibodies may be performed at a minimum dilution of 1 : 100. |
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就是蛋白质分子的小片断
是氨基酸形成的
求助各位前辈，我最近在合成的化合物水溶性很好，非常好，以至于可以随便溶解在水里，它的六氟磷酸盐也可以随意溶解在水里（大于50uM），细胞成像实验显示它根本进不去细胞，求问有没有啥方法包裹一下让它进去？我搜了一下文献，感觉多数是把脂溶性特别好的东西包裹一下弄进去的，也许是搜索姿势不对没找到我需要的答案，**点拨啊！！！
如题，之前没做过药代，老师给了一个600+Da的五肽，想测下药代动参数，看文献推荐上述两种方法，但是不知道选哪种更好，lcms前处理会不会影响小肽。
有机的是有机化合物的简称,它指的是含碳化合物.
但是,有四大类常见物质一般不作为有机物处理：
1、碳的氧化物,如CO和CO2.
2、碳酸及其盐,如CaCO3.
3、金属碳化物,如CaC2.
4、拟卤素及其化合物,如(CN)2与KSCN.
水的化学式为H2O,它不含有碳元素,故不是有机物.
但若所描述的水不是化学意义的水,而是自然界存在的天然水,那么,水中会溶有一定量的有机物.
