Product Specifications:
Item# 1004 : Recombinant HIV-1 IIIB Glycoprotein Soluble gp160
Concentration: See Vial
Mass/Vial: 100µg
Diluent: PBS In-Vitro Diagnostics
Purity: >95%
Stabilizer: None
Preservative: None
Storage: -75°C
Physical State: Frozen Liquid
Stability: 2 years at -75°C.
Application: CD4 Binding, Vaccines, T- Cell Activation, In- Vitro Diagonostics.
Description: Full length Recombinant HIV-1 IIIB soluble - membrane anchor sequence deleted- gp160 glycoprotein produced in the Baculovirus Expression System.
Purification: This protein is purified by immunoaffinity Chromatography to >95% purity as determined by SDS-PAGE, reduced.
Specificity: This protein binds to murine monoclonal antibodies of defined epitope specificit and human serum polyclonal antibodies in ELISA and Western ELISA.
Biological Activity: This protein binds to human T-cell receptor CD4 in ELISA and Western ELISA as determined by CD4/gp160/Anti gp160 mAb-peroxidase capture ELISA. This protein activates human T-Lymphocytes (CD4+, CD4-), in vitro, as measured by RNA synthesis during G0 to G1 transition phase of antigen-binding competent cells.
Applications and Instructions for use
Recommended concentrations for use are approximate values. A dose dependent response assay should be performed to determine the optimal concentration for use in specific applications. ELISA and Western ELISA require 10-100ng protein depending on the nature and affinity of the detection reagent. HIV-1 converted human serum polyclonal antibodies yield titers of 1:1000 or greater at 1-10ng of immobilized protein under standard ELISA conditions.
Item#: 1004-P Peroxidase-Conjugated rgp160 HIV-1 IIIB
Application: Human diagnostics, CD4 binding, Drug screening, Human T-cell CD4 studies.
Item#: 1004-G Colloidal gold-Conjugated rgp160 HIV-1 IIIB
Application: Human diagnostics, CD4 binding, Drug screening, FACS analysis.
Item#: 1004-F Flourescene-Conjugated rgp160 HIV-1 III
Application: FACS analysis, Histocytology.
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如题，实验小白，但是导师给了这个课题，对于这通路不是很了解，园子里也没找到关于非经典通路的，希望各位战友帮忙下，谢谢了
我最近跑电泳NF-kB信号通路，跑了p65和IkB-α两个蛋白，p65显影的时候有结果，但是IkB-α显示的时候是没有条带，空白一片，我跑电泳的条件不变、蛋白样品也是相同的，但是一个有结果，一个没有结果，请问原因是什么，请大神帮忙解答一下，谢谢啦~~
细胞信号转导分析的生物信息学方法:
a)选择关键词，从GO数据库中寻找相关基因，比如extracellular表示为分泌蛋白
b)通过GO,BioCarta和Kegg信号通路数据分析给定基因所属的信号通路，功能分类等
c)比较多组基因按功能，通路分组在统计学上的差异，从而得到各组基因的功能差异
d)新信号通路的分析
回复：【资源】很有价值的信号通路分析平台方法!-丁香园论坛
我想测药物干预下P65信号通路激活情况，想问下需要像wb一样做一个磷酸化/总蛋白的比值吗，还是分别测出来比较就可以了呢。
